Sphaerotilus hippei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Sphaerotilus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A318GY57|A0A318GY57_9BURK Iron-sulfur cluster assembly protein OS=Sphaerotilus hippei OX=744406 GN=C7444_1148 PE=3 SV=1
GG1 pKa = 6.14TVVEE5 pKa = 4.82GEE7 pKa = 4.31VATGYY12 pKa = 8.17TVQLSQPAATDD23 pKa = 3.32LTVQLTYY30 pKa = 11.05SGTATDD36 pKa = 4.1GSDD39 pKa = 3.35YY40 pKa = 10.56TKK42 pKa = 10.54VVSVTVPAGSASTTFDD58 pKa = 2.94IATIDD63 pKa = 5.06DD64 pKa = 3.96ALADD68 pKa = 3.76NGEE71 pKa = 4.48SFTVTLGTITGGGFEE86 pKa = 4.66AVAVDD91 pKa = 3.79PTTASVTTVISDD103 pKa = 3.56DD104 pKa = 3.78VGPGVPGGGTPGTEE118 pKa = 3.97DD119 pKa = 3.32TVTISLTGPGTVVEE133 pKa = 4.59GQVATGYY140 pKa = 8.16TVQLSQPAATDD151 pKa = 3.32LTFQLTYY158 pKa = 10.89SGTATDD164 pKa = 4.1GSDD167 pKa = 3.4YY168 pKa = 10.89TKK170 pKa = 10.55VISVTVPAGSASTTFDD186 pKa = 2.75ITTLDD191 pKa = 4.7DD192 pKa = 4.07ALADD196 pKa = 3.65NGEE199 pKa = 4.48SFTVTLGTITGGGFEE214 pKa = 4.94SVAADD219 pKa = 3.55PTAASVTTVISDD231 pKa = 3.56DD232 pKa = 3.78VGPGVPGGGTPGTEE246 pKa = 3.97DD247 pKa = 3.32TVTISLTGPGTVVEE261 pKa = 4.74GEE263 pKa = 4.27VATGYY268 pKa = 8.2TVHH271 pKa = 7.22LSQPAATDD279 pKa = 3.32LTVQLTYY286 pKa = 11.05SGTATDD292 pKa = 4.1GSDD295 pKa = 3.35YY296 pKa = 10.56TKK298 pKa = 10.54VVSVTVPAGSASTSFDD314 pKa = 2.76ITTLDD319 pKa = 4.97DD320 pKa = 4.07ALADD324 pKa = 3.65NGEE327 pKa = 4.48SFTVTLGTITGGGFEE342 pKa = 4.66AVAVDD347 pKa = 3.78PTAASVTTVIADD359 pKa = 3.57DD360 pKa = 4.05VGPGVPGGGTPGAEE374 pKa = 3.93DD375 pKa = 3.71TVTISLTGPGTVVEE389 pKa = 4.59GQVATGYY396 pKa = 8.16TVQLSQPAATDD407 pKa = 3.32LTVQLTYY414 pKa = 11.05SGTATDD420 pKa = 4.1GSDD423 pKa = 3.35YY424 pKa = 10.56TKK426 pKa = 10.54VVSVTVPAGSASTSFDD442 pKa = 4.16LATLDD447 pKa = 3.93DD448 pKa = 4.67ALAEE452 pKa = 4.42GSEE455 pKa = 4.58SVTVTLGTITGGGFEE470 pKa = 4.66AVAVDD475 pKa = 3.78PTAASVTTVIADD487 pKa = 3.57DD488 pKa = 4.05VGPGVPGGGTPGAEE502 pKa = 3.93DD503 pKa = 3.71TVTISLTGPGSVVEE517 pKa = 4.53GEE519 pKa = 4.35VATGYY524 pKa = 8.2TVHH527 pKa = 7.22LSQPAATDD535 pKa = 3.32LTVQLTYY542 pKa = 11.05SGTATDD548 pKa = 4.1GSDD551 pKa = 3.35YY552 pKa = 10.56TKK554 pKa = 10.54VVSVTVPAGSASTSFDD570 pKa = 4.16LATLDD575 pKa = 3.93DD576 pKa = 4.67ALAEE580 pKa = 4.42GSEE583 pKa = 4.58SVTVTLGAVTGGGFEE598 pKa = 4.24AVAVDD603 pKa = 3.88PTAVSVTTVISDD615 pKa = 3.56DD616 pKa = 3.78VGPGVPGGGTPGTEE630 pKa = 3.97DD631 pKa = 3.32TVTISLTGPGTVVEE645 pKa = 4.74GEE647 pKa = 4.27VATGYY652 pKa = 8.2TVHH655 pKa = 7.22LSQPAATDD663 pKa = 3.32LTVQLTYY670 pKa = 11.05SGTATDD676 pKa = 4.1GSDD679 pKa = 3.4YY680 pKa = 10.89TKK682 pKa = 10.55VISVTVPAGSASTSFDD698 pKa = 4.16LATLDD703 pKa = 3.93DD704 pKa = 4.67ALAEE708 pKa = 4.42GSEE711 pKa = 4.58SVTVTLGAVTGGGFEE726 pKa = 4.24AVAVDD731 pKa = 3.78PTAASVTTVIADD743 pKa = 3.61DD744 pKa = 4.16VGPGIPGGGTPGAEE758 pKa = 3.76DD759 pKa = 3.69TVNISLTGPGTVVEE773 pKa = 4.74GEE775 pKa = 4.27VATGYY780 pKa = 8.2TVHH783 pKa = 7.22LSQPAATDD791 pKa = 3.32LTVQLTYY798 pKa = 11.05SGTATDD804 pKa = 4.1GSDD807 pKa = 3.35YY808 pKa = 10.56TKK810 pKa = 10.54VVSVTVPAGSASTSFDD826 pKa = 4.16LATLDD831 pKa = 3.93DD832 pKa = 4.67ALAEE836 pKa = 4.42GSEE839 pKa = 4.58SVTVTLGTITGGGFEE854 pKa = 4.66AVAVDD859 pKa = 3.99PTAGSITTVIVDD871 pKa = 4.5DD872 pKa = 4.77VGPDD876 pKa = 3.4APGVKK881 pKa = 9.49PGPEE885 pKa = 3.91DD886 pKa = 3.42TVSISLTGPGTVVEE900 pKa = 4.74GEE902 pKa = 4.27VATGYY907 pKa = 8.17TVQLSQPAATDD918 pKa = 3.32LTVQLTYY925 pKa = 11.05SGTATDD931 pKa = 4.1GSDD934 pKa = 3.4YY935 pKa = 10.89TKK937 pKa = 10.55VISVTVPAGSASTTFDD953 pKa = 2.75ITTLDD958 pKa = 4.7DD959 pKa = 4.07ALADD963 pKa = 3.65NGEE966 pKa = 4.48SFTVTLGAVTGGGFEE981 pKa = 4.24AVAVDD986 pKa = 3.78PTAASVTTVISDD998 pKa = 4.13DD999 pKa = 3.76IGPGVPGGGMPGTEE1013 pKa = 3.99DD1014 pKa = 3.3TVTIGLTGPGTVVEE1028 pKa = 4.74GEE1030 pKa = 4.27VATGYY1035 pKa = 8.2TVHH1038 pKa = 7.22LSQPAATDD1046 pKa = 3.32LTVQLTYY1053 pKa = 11.05SGTATDD1059 pKa = 4.1GSDD1062 pKa = 3.35YY1063 pKa = 10.56TKK1065 pKa = 10.54VVSVTVPAGSASSSFDD1081 pKa = 4.13LATLDD1086 pKa = 3.93DD1087 pKa = 4.67ALAEE1091 pKa = 4.42GSEE1094 pKa = 4.58SVTVTLGAVTGGGFEE1109 pKa = 4.24AVAVDD1114 pKa = 3.78PTAASVTTVISDD1126 pKa = 3.56DD1127 pKa = 3.78VGPGVPGGGTPGSEE1141 pKa = 4.03DD1142 pKa = 3.52TVTISLTGPGTVVEE1156 pKa = 4.74GEE1158 pKa = 4.27VATGYY1163 pKa = 8.28TVNLSQPAATDD1174 pKa = 3.32LTVQLTYY1181 pKa = 11.05SGTATDD1187 pKa = 4.1GSDD1190 pKa = 3.4YY1191 pKa = 10.89TKK1193 pKa = 10.55VISVTVPAGSASTSFDD1209 pKa = 4.16LATLDD1214 pKa = 3.93DD1215 pKa = 4.67ALAEE1219 pKa = 4.42GSEE1222 pKa = 4.58SVTVTLGAVTGGGFEE1237 pKa = 4.56TIAVDD1242 pKa = 3.43PTTASITTVISDD1254 pKa = 3.74DD1255 pKa = 3.78VGPGVPGGGTPGTQDD1270 pKa = 2.89TVTISLTGPGTVVEE1284 pKa = 4.74GEE1286 pKa = 4.27VATGYY1291 pKa = 8.17TVQLSQPAATDD1302 pKa = 3.32LTVQLTYY1309 pKa = 11.05SGTATDD1315 pKa = 4.1GSDD1318 pKa = 3.4YY1319 pKa = 10.89TKK1321 pKa = 10.55VISVTVPAGSASTSFDD1337 pKa = 2.76ITTLDD1342 pKa = 4.97DD1343 pKa = 4.07ALADD1347 pKa = 3.65NGEE1350 pKa = 4.48SFTVTLGAVTGGGFEE1365 pKa = 4.2AVAVDD1370 pKa = 4.35PVASSVTTIISDD1382 pKa = 3.65DD1383 pKa = 3.82VGPGVPGGGTPGAEE1397 pKa = 3.93DD1398 pKa = 3.71TVTISLTGPGTVVEE1412 pKa = 4.66GEE1414 pKa = 4.48VACGYY1419 pKa = 7.66TVHH1422 pKa = 7.23LSQPAASEE1430 pKa = 4.29VTVEE1434 pKa = 4.76LIYY1437 pKa = 11.1SGTATDD1443 pKa = 5.02GSDD1446 pKa = 3.22FSKK1449 pKa = 10.78VVSVTIPAGSASADD1463 pKa = 3.41FDD1465 pKa = 3.67IATIDD1470 pKa = 3.52DD1471 pKa = 4.02ALVEE1475 pKa = 4.29RR1476 pKa = 11.84GEE1478 pKa = 4.25SFIVGIGGTSGGGFEE1493 pKa = 5.0ALAVDD1498 pKa = 4.37PVANAVTTAIVDD1510 pKa = 3.94DD1511 pKa = 5.29AGPAAPGGPGAPGVEE1526 pKa = 4.2DD1527 pKa = 3.66TVLVSISGPGSVIEE1541 pKa = 4.6GEE1543 pKa = 4.33TASGYY1548 pKa = 7.28TVCLSHH1554 pKa = 7.36AAATDD1559 pKa = 3.37MTVQLIYY1566 pKa = 10.64TGTATNGEE1574 pKa = 4.26DD1575 pKa = 3.42FQAVVSVTIPAGSASAAFEE1594 pKa = 4.49VPTLDD1599 pKa = 4.61DD1600 pKa = 3.95ALAEE1604 pKa = 4.09VGEE1607 pKa = 4.46RR1608 pKa = 11.84FTIAVGGTTGGGFEE1622 pKa = 4.46EE1623 pKa = 4.92VAVDD1627 pKa = 3.82PAANAVTTTIVDD1639 pKa = 3.45DD1640 pKa = 5.16AGPDD1644 pKa = 3.85LPPGSPPAGPEE1655 pKa = 3.75DD1656 pKa = 3.69TVLIGISGPDD1666 pKa = 3.09QVGEE1670 pKa = 4.42GEE1672 pKa = 4.37VATGYY1677 pKa = 9.93VVHH1680 pKa = 7.02LSQAAVTDD1688 pKa = 3.57LTVQLTYY1695 pKa = 11.13GGTATDD1701 pKa = 3.7GSDD1704 pKa = 3.5YY1705 pKa = 10.7MRR1707 pKa = 11.84VATVTIPAGASSVGFDD1723 pKa = 5.0LATLPDD1729 pKa = 4.31GQPEE1733 pKa = 4.08GAEE1736 pKa = 4.42CIVIGIGATHH1746 pKa = 6.73GGGFEE1751 pKa = 4.02VLAVDD1756 pKa = 4.49GSAAQVVTAVVDD1768 pKa = 3.68PATAPQISGPLDD1780 pKa = 3.28VRR1782 pKa = 11.84VSEE1785 pKa = 4.42EE1786 pKa = 3.78GLAQGLADD1794 pKa = 3.74GTGARR1799 pKa = 11.84AGDD1802 pKa = 3.55DD1803 pKa = 3.53TGNSRR1808 pKa = 11.84SVHH1811 pKa = 5.13GQLTLSDD1818 pKa = 4.22ADD1820 pKa = 3.85SASLQVTLTAPTTGLSCGGQPVVWTGDD1847 pKa = 3.35GTGTLVGHH1855 pKa = 7.07IGSATGAVALSITIDD1870 pKa = 3.02ASGAYY1875 pKa = 9.84DD1876 pKa = 3.58VTLDD1880 pKa = 3.81AALDD1884 pKa = 3.7HH1885 pKa = 6.84ALQGEE1890 pKa = 4.56DD1891 pKa = 4.92LLSLDD1896 pKa = 3.21VGVRR1900 pKa = 11.84VSDD1903 pKa = 3.74GTHH1906 pKa = 6.21TSTATLTVGIEE1917 pKa = 4.12DD1918 pKa = 4.32DD1919 pKa = 4.51APTASHH1925 pKa = 6.0STATLLVRR1933 pKa = 11.84HH1934 pKa = 6.28DD1935 pKa = 4.84SIRR1938 pKa = 11.84ISQMDD1943 pKa = 3.72AGFSDD1948 pKa = 3.45DD1949 pKa = 3.48TYY1951 pKa = 11.51RR1952 pKa = 11.84NNDD1955 pKa = 3.45CDD1957 pKa = 3.81VSRR1960 pKa = 11.84SDD1962 pKa = 4.0TDD1964 pKa = 3.45SHH1966 pKa = 7.36DD1967 pKa = 3.74GNIDD1971 pKa = 3.38QLRR1974 pKa = 11.84WGQAANDD1981 pKa = 3.72QNRR1984 pKa = 11.84QSGYY1988 pKa = 11.05DD1989 pKa = 3.53LVDD1992 pKa = 3.38STRR1995 pKa = 11.84LTTATGATVEE2005 pKa = 4.01AGEE2008 pKa = 4.33VFKK2011 pKa = 11.13LADD2014 pKa = 3.64FTHH2017 pKa = 6.95RR2018 pKa = 11.84NYY2020 pKa = 11.0AIASGSSTLKK2030 pKa = 9.83EE2031 pKa = 3.87TDD2033 pKa = 3.8LDD2035 pKa = 3.43LTMAVEE2041 pKa = 4.29INGVSTQVTFTAHH2054 pKa = 6.83LVHH2057 pKa = 6.72TEE2059 pKa = 4.16TPNSSDD2065 pKa = 2.91AVASRR2070 pKa = 11.84DD2071 pKa = 3.79IIQLPAQTVTVQVNGQDD2088 pKa = 3.68YY2089 pKa = 10.22EE2090 pKa = 4.6VNLLGFKK2097 pKa = 10.52DD2098 pKa = 3.71SSGQLLTEE2106 pKa = 3.74IRR2108 pKa = 11.84TDD2110 pKa = 3.19EE2111 pKa = 4.11NTASNTFGLYY2121 pKa = 10.75GSVTPTDD2128 pKa = 4.25LPPDD2132 pKa = 3.47LVGQVQVDD2140 pKa = 3.83AGADD2144 pKa = 3.38GLAAIRR2150 pKa = 11.84WSDD2153 pKa = 3.08TGSAYY2158 pKa = 9.43GTFTGDD2164 pKa = 3.12ADD2166 pKa = 3.69GSYY2169 pKa = 9.52TFVMNQATRR2178 pKa = 11.84EE2179 pKa = 3.98ALAASGEE2186 pKa = 4.18ALTQTFTYY2194 pKa = 10.42QVVDD2198 pKa = 3.39RR2199 pKa = 11.84DD2200 pKa = 3.68GDD2202 pKa = 3.92VTSGSVTVALARR2214 pKa = 11.84EE2215 pKa = 4.17EE2216 pKa = 4.01TLAPVTLTSTTQTIDD2231 pKa = 3.01SDD2233 pKa = 4.59SIGLGGEE2240 pKa = 3.94YY2241 pKa = 10.06FGYY2244 pKa = 10.61NDD2246 pKa = 3.46NRR2248 pKa = 11.84TGTAHH2253 pKa = 7.75DD2254 pKa = 3.89ATFSGATRR2262 pKa = 11.84LHH2264 pKa = 6.71ADD2266 pKa = 4.37DD2267 pKa = 5.7GSATLGGNTSTNLNHH2282 pKa = 7.04LADD2285 pKa = 3.92IEE2287 pKa = 4.78HH2288 pKa = 6.44IVEE2291 pKa = 4.18GRR2293 pKa = 11.84NGNTDD2298 pKa = 3.44LVGNAQLSLAGAADD2312 pKa = 3.46ATFTMRR2318 pKa = 11.84NVEE2321 pKa = 4.28FGLVAGTDD2329 pKa = 3.31QALFSTDD2336 pKa = 3.53LGQSGSTFASGQTVTGNNLDD2356 pKa = 3.91RR2357 pKa = 11.84FLHH2360 pKa = 6.35GSNAGNASAITATSGMGDD2378 pKa = 3.3STDD2381 pKa = 3.8AIIRR2385 pKa = 11.84AVGYY2389 pKa = 10.22IDD2391 pKa = 3.72MTGGTYY2397 pKa = 10.03DD2398 pKa = 3.41IRR2400 pKa = 11.84VTGDD2404 pKa = 2.96DD2405 pKa = 4.38GYY2407 pKa = 11.6RR2408 pKa = 11.84LLIGGEE2414 pKa = 4.34TVAEE2418 pKa = 4.02VDD2420 pKa = 3.62VNQYY2424 pKa = 9.5VAVRR2428 pKa = 11.84TFTGVDD2434 pKa = 3.71LSDD2437 pKa = 3.54GLQPIEE2443 pKa = 4.44ILYY2446 pKa = 9.3WDD2448 pKa = 3.9QGGHH2452 pKa = 5.48ASLRR2456 pKa = 11.84IEE2458 pKa = 4.73LKK2460 pKa = 10.55PSGAADD2466 pKa = 3.32TDD2468 pKa = 4.24YY2469 pKa = 11.44KK2470 pKa = 11.52VLGSDD2475 pKa = 4.2EE2476 pKa = 4.39YY2477 pKa = 11.93ALFAPGEE2484 pKa = 4.33VPVLGAQQDD2493 pKa = 3.73IVEE2496 pKa = 4.31SSTNGVWEE2504 pKa = 3.87IRR2506 pKa = 11.84TGQDD2510 pKa = 3.16YY2511 pKa = 9.97TGTSGNDD2518 pKa = 3.02QVTGSDD2524 pKa = 3.29GKK2526 pKa = 10.68DD2527 pKa = 3.4VIRR2530 pKa = 11.84GGAGRR2535 pKa = 11.84DD2536 pKa = 3.83TIDD2539 pKa = 3.83GAQGDD2544 pKa = 4.12DD2545 pKa = 3.47RR2546 pKa = 11.84LEE2548 pKa = 4.48GGSGHH2553 pKa = 7.3DD2554 pKa = 3.91VLTGGQGADD2563 pKa = 3.09VFAWSLADD2571 pKa = 4.03AGSAGAPAHH2580 pKa = 6.32DD2581 pKa = 3.82TVTDD2585 pKa = 4.19FKK2587 pKa = 11.17VATVGGDD2594 pKa = 3.48ALDD2597 pKa = 5.36LRR2599 pKa = 11.84DD2600 pKa = 4.03LLQGEE2605 pKa = 4.2NHH2607 pKa = 6.95ANLTNYY2613 pKa = 10.4LHH2615 pKa = 7.08FEE2617 pKa = 4.24TSGSDD2622 pKa = 3.25TVVSISSTGGFAGGYY2637 pKa = 9.6DD2638 pKa = 3.96SSHH2641 pKa = 6.51TDD2643 pKa = 2.99QTITLSGVTLGGGSDD2658 pKa = 3.4MSVVLDD2664 pKa = 4.49LLAKK2668 pKa = 9.96NQLVVDD2674 pKa = 4.18NGG2676 pKa = 3.53

Molecular weight:
264.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A318H9R7|A0A318H9R7_9BURK Aryl-alcohol dehydrogenase-like predicted oxidoreductase OS=Sphaerotilus hippei OX=744406 GN=C7444_11035 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 10.03TRR25 pKa = 11.84GGRR28 pKa = 11.84QVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3942

0

3942

1352833

29

5050

343.2

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.013 ± 0.048

0.963 ± 0.013

5.448 ± 0.032

5.246 ± 0.032

3.104 ± 0.024

8.461 ± 0.04

2.483 ± 0.022

3.852 ± 0.027

2.365 ± 0.035

11.515 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.019

2.041 ± 0.024

5.528 ± 0.042

4.143 ± 0.033

7.829 ± 0.051

5.347 ± 0.043

5.166 ± 0.063

7.747 ± 0.04

1.549 ± 0.019

1.875 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski