Amino acid dipepetide frequency for Actinomadura sp. NEAU-G17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.456AlaAla: 22.456 ± 0.16
1.024AlaCys: 1.024 ± 0.022
8.874AlaAsp: 8.874 ± 0.072
8.92AlaGlu: 8.92 ± 0.079
3.832AlaPhe: 3.832 ± 0.044
13.547AlaGly: 13.547 ± 0.1
2.844AlaHis: 2.844 ± 0.04
3.58AlaIle: 3.58 ± 0.05
2.573AlaLys: 2.573 ± 0.047
14.829AlaLeu: 14.829 ± 0.112
2.628AlaMet: 2.628 ± 0.038
1.912AlaAsn: 1.912 ± 0.03
7.288AlaPro: 7.288 ± 0.065
3.065AlaGln: 3.065 ± 0.043
11.616AlaArg: 11.616 ± 0.081
6.489AlaSer: 6.489 ± 0.061
6.593AlaThr: 6.593 ± 0.051
12.652AlaVal: 12.652 ± 0.094
1.994AlaTrp: 1.994 ± 0.031
2.558AlaTyr: 2.558 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.047CysAla: 1.047 ± 0.021
0.081CysCys: 0.081 ± 0.006
0.481CysAsp: 0.481 ± 0.018
0.388CysGlu: 0.388 ± 0.014
0.209CysPhe: 0.209 ± 0.01
0.959CysGly: 0.959 ± 0.023
0.195CysHis: 0.195 ± 0.01
0.12CysIle: 0.12 ± 0.007
0.106CysLys: 0.106 ± 0.008
0.705CysLeu: 0.705 ± 0.019
0.126CysMet: 0.126 ± 0.008
0.112CysAsn: 0.112 ± 0.007
0.527CysPro: 0.527 ± 0.017
0.159CysGln: 0.159 ± 0.009
0.606CysArg: 0.606 ± 0.015
0.401CysSer: 0.401 ± 0.016
0.423CysThr: 0.423 ± 0.015
0.666CysVal: 0.666 ± 0.017
0.1CysTrp: 0.1 ± 0.007
0.165CysTyr: 0.165 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.355AspAla: 8.355 ± 0.068
0.396AspCys: 0.396 ± 0.015
3.747AspAsp: 3.747 ± 0.045
3.995AspGlu: 3.995 ± 0.047
1.541AspPhe: 1.541 ± 0.027
6.789AspGly: 6.789 ± 0.065
1.42AspHis: 1.42 ± 0.028
1.629AspIle: 1.629 ± 0.031
0.981AspLys: 0.981 ± 0.023
6.716AspLeu: 6.716 ± 0.06
0.888AspMet: 0.888 ± 0.023
0.787AspAsn: 0.787 ± 0.017
4.821AspPro: 4.821 ± 0.053
1.471AspGln: 1.471 ± 0.027
5.574AspArg: 5.574 ± 0.063
1.939AspSer: 1.939 ± 0.028
2.662AspThr: 2.662 ± 0.041
5.173AspVal: 5.173 ± 0.047
0.919AspTrp: 0.919 ± 0.02
1.045AspTyr: 1.045 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.14GluAla: 7.14 ± 0.073
0.349GluCys: 0.349 ± 0.014
2.764GluAsp: 2.764 ± 0.041
3.059GluGlu: 3.059 ± 0.045
1.486GluPhe: 1.486 ± 0.029
3.954GluGly: 3.954 ± 0.044
1.58GluHis: 1.58 ± 0.03
2.297GluIle: 2.297 ± 0.032
1.174GluLys: 1.174 ± 0.027
6.542GluLeu: 6.542 ± 0.068
0.941GluMet: 0.941 ± 0.023
0.928GluAsn: 0.928 ± 0.021
3.549GluPro: 3.549 ± 0.04
1.776GluGln: 1.776 ± 0.031
5.931GluArg: 5.931 ± 0.06
2.263GluSer: 2.263 ± 0.039
2.892GluThr: 2.892 ± 0.04
4.481GluVal: 4.481 ± 0.055
0.737GluTrp: 0.737 ± 0.018
1.061GluTyr: 1.061 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.934PheAla: 3.934 ± 0.045
0.275PheCys: 0.275 ± 0.012
1.991PheAsp: 1.991 ± 0.036
1.433PheGlu: 1.433 ± 0.028
0.847PhePhe: 0.847 ± 0.023
3.283PheGly: 3.283 ± 0.042
0.612PheHis: 0.612 ± 0.017
0.586PheIle: 0.586 ± 0.016
0.462PheLys: 0.462 ± 0.016
2.628PheLeu: 2.628 ± 0.037
0.421PheMet: 0.421 ± 0.014
0.513PheAsn: 0.513 ± 0.016
1.457PhePro: 1.457 ± 0.025
0.711PheGln: 0.711 ± 0.017
1.975PheArg: 1.975 ± 0.034
1.362PheSer: 1.362 ± 0.025
1.985PheThr: 1.985 ± 0.032
2.447PheVal: 2.447 ± 0.037
0.44PheTrp: 0.44 ± 0.015
0.585PheTyr: 0.585 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.404GlyAla: 11.404 ± 0.093
0.811GlyCys: 0.811 ± 0.024
5.502GlyAsp: 5.502 ± 0.053
5.063GlyGlu: 5.063 ± 0.048
2.939GlyPhe: 2.939 ± 0.034
9.875GlyGly: 9.875 ± 0.094
2.364GlyHis: 2.364 ± 0.037
3.056GlyIle: 3.056 ± 0.039
2.091GlyLys: 2.091 ± 0.035
9.776GlyLeu: 9.776 ± 0.082
2.096GlyMet: 2.096 ± 0.027
1.559GlyAsn: 1.559 ± 0.034
5.748GlyPro: 5.748 ± 0.06
2.355GlyGln: 2.355 ± 0.043
9.131GlyArg: 9.131 ± 0.079
4.91GlySer: 4.91 ± 0.06
6.105GlyThr: 6.105 ± 0.061
8.114GlyVal: 8.114 ± 0.071
1.74GlyTrp: 1.74 ± 0.03
2.197GlyTyr: 2.197 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.74HisAla: 2.74 ± 0.033
0.204HisCys: 0.204 ± 0.009
1.403HisAsp: 1.403 ± 0.027
1.133HisGlu: 1.133 ± 0.022
0.598HisPhe: 0.598 ± 0.017
2.446HisGly: 2.446 ± 0.042
0.625HisHis: 0.625 ± 0.018
0.62HisIle: 0.62 ± 0.017
0.282HisLys: 0.282 ± 0.012
2.337HisLeu: 2.337 ± 0.032
0.381HisMet: 0.381 ± 0.015
0.328HisAsn: 0.328 ± 0.011
1.772HisPro: 1.772 ± 0.027
0.544HisGln: 0.544 ± 0.015
2.147HisArg: 2.147 ± 0.034
0.881HisSer: 0.881 ± 0.019
1.133HisThr: 1.133 ± 0.025
1.797HisVal: 1.797 ± 0.029
0.372HisTrp: 0.372 ± 0.014
0.477HisTyr: 0.477 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.555IleAla: 4.555 ± 0.046
0.264IleCys: 0.264 ± 0.01
2.092IleAsp: 2.092 ± 0.033
1.816IleGlu: 1.816 ± 0.035
0.671IlePhe: 0.671 ± 0.02
3.251IleGly: 3.251 ± 0.039
0.53IleHis: 0.53 ± 0.017
0.882IleIle: 0.882 ± 0.022
0.604IleLys: 0.604 ± 0.019
2.339IleLeu: 2.339 ± 0.036
0.466IleMet: 0.466 ± 0.015
0.613IleAsn: 0.613 ± 0.019
1.642IlePro: 1.642 ± 0.029
0.657IleGln: 0.657 ± 0.02
2.251IleArg: 2.251 ± 0.036
1.457IleSer: 1.457 ± 0.027
2.085IleThr: 2.085 ± 0.036
2.913IleVal: 2.913 ± 0.042
0.358IleTrp: 0.358 ± 0.014
0.488IleTyr: 0.488 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.555LysAla: 2.555 ± 0.047
0.108LysCys: 0.108 ± 0.007
1.09LysAsp: 1.09 ± 0.026
0.908LysGlu: 0.908 ± 0.025
0.409LysPhe: 0.409 ± 0.013
1.633LysGly: 1.633 ± 0.033
0.386LysHis: 0.386 ± 0.013
0.81LysIle: 0.81 ± 0.023
0.643LysLys: 0.643 ± 0.025
1.738LysLeu: 1.738 ± 0.032
0.355LysMet: 0.355 ± 0.013
0.436LysAsn: 0.436 ± 0.016
1.283LysPro: 1.283 ± 0.028
0.566LysGln: 0.566 ± 0.016
1.388LysArg: 1.388 ± 0.032
0.98LysSer: 0.98 ± 0.025
1.093LysThr: 1.093 ± 0.029
1.752LysVal: 1.752 ± 0.033
0.229LysTrp: 0.229 ± 0.011
0.376LysTyr: 0.376 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
16.04LeuAla: 16.04 ± 0.106
0.766LeuCys: 0.766 ± 0.018
6.859LeuAsp: 6.859 ± 0.066
4.7LeuGlu: 4.7 ± 0.056
2.689LeuPhe: 2.689 ± 0.042
9.611LeuGly: 9.611 ± 0.075
2.114LeuHis: 2.114 ± 0.033
2.792LeuIle: 2.792 ± 0.041
1.696LeuLys: 1.696 ± 0.032
11.143LeuLeu: 11.143 ± 0.103
1.653LeuMet: 1.653 ± 0.026
1.667LeuAsn: 1.667 ± 0.031
6.624LeuPro: 6.624 ± 0.055
1.857LeuGln: 1.857 ± 0.03
9.263LeuArg: 9.263 ± 0.072
4.909LeuSer: 4.909 ± 0.049
6.711LeuThr: 6.711 ± 0.059
9.454LeuVal: 9.454 ± 0.077
1.278LeuTrp: 1.278 ± 0.026
1.806LeuTyr: 1.806 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.389MetAla: 2.389 ± 0.034
0.131MetCys: 0.131 ± 0.008
0.95MetAsp: 0.95 ± 0.023
0.759MetGlu: 0.759 ± 0.019
0.53MetPhe: 0.53 ± 0.016
1.325MetGly: 1.325 ± 0.026
0.32MetHis: 0.32 ± 0.011
0.715MetIle: 0.715 ± 0.016
0.402MetLys: 0.402 ± 0.014
1.865MetLeu: 1.865 ± 0.028
0.327MetMet: 0.327 ± 0.013
0.407MetAsn: 0.407 ± 0.012
1.208MetPro: 1.208 ± 0.024
0.389MetGln: 0.389 ± 0.014
1.664MetArg: 1.664 ± 0.028
1.26MetSer: 1.26 ± 0.027
1.455MetThr: 1.455 ± 0.027
1.458MetVal: 1.458 ± 0.026
0.217MetTrp: 0.217 ± 0.01
0.278MetTyr: 0.278 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.232AsnAla: 2.232 ± 0.038
0.154AsnCys: 0.154 ± 0.009
0.865AsnAsp: 0.865 ± 0.019
0.764AsnGlu: 0.764 ± 0.018
0.434AsnPhe: 0.434 ± 0.015
1.791AsnGly: 1.791 ± 0.035
0.365AsnHis: 0.365 ± 0.014
0.566AsnIle: 0.566 ± 0.016
0.338AsnLys: 0.338 ± 0.014
1.662AsnLeu: 1.662 ± 0.03
0.293AsnMet: 0.293 ± 0.013
0.327AsnAsn: 0.327 ± 0.014
1.314AsnPro: 1.314 ± 0.028
0.428AsnGln: 0.428 ± 0.015
1.265AsnArg: 1.265 ± 0.025
0.71AsnSer: 0.71 ± 0.021
0.943AsnThr: 0.943 ± 0.023
1.433AsnVal: 1.433 ± 0.029
0.252AsnTrp: 0.252 ± 0.01
0.379AsnTyr: 0.379 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.978ProAla: 8.978 ± 0.08
0.369ProCys: 0.369 ± 0.011
5.245ProAsp: 5.245 ± 0.054
4.282ProGlu: 4.282 ± 0.049
1.63ProPhe: 1.63 ± 0.026
7.177ProGly: 7.177 ± 0.068
1.396ProHis: 1.396 ± 0.025
1.384ProIle: 1.384 ± 0.025
1.18ProLys: 1.18 ± 0.027
5.407ProLeu: 5.407 ± 0.055
1.065ProMet: 1.065 ± 0.021
1.004ProAsn: 1.004 ± 0.024
4.368ProPro: 4.368 ± 0.062
1.464ProGln: 1.464 ± 0.026
4.524ProArg: 4.524 ± 0.048
3.795ProSer: 3.795 ± 0.054
3.092ProThr: 3.092 ± 0.04
5.643ProVal: 5.643 ± 0.05
0.952ProTrp: 0.952 ± 0.021
1.299ProTyr: 1.299 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.255GlnAla: 3.255 ± 0.041
0.152GlnCys: 0.152 ± 0.009
1.213GlnAsp: 1.213 ± 0.024
1.143GlnGlu: 1.143 ± 0.027
0.626GlnPhe: 0.626 ± 0.018
2.069GlnGly: 2.069 ± 0.034
0.522GlnHis: 0.522 ± 0.014
1.016GlnIle: 1.016 ± 0.021
0.536GlnLys: 0.536 ± 0.017
2.256GlnLeu: 2.256 ± 0.033
0.442GlnMet: 0.442 ± 0.015
0.481GlnAsn: 0.481 ± 0.016
1.446GlnPro: 1.446 ± 0.034
0.915GlnGln: 0.915 ± 0.031
2.145GlnArg: 2.145 ± 0.037
1.044GlnSer: 1.044 ± 0.024
1.157GlnThr: 1.157 ± 0.025
2.228GlnVal: 2.228 ± 0.039
0.369GlnTrp: 0.369 ± 0.011
0.469GlnTyr: 0.469 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
11.132ArgAla: 11.132 ± 0.085
0.605ArgCys: 0.605 ± 0.018
5.207ArgAsp: 5.207 ± 0.059
4.991ArgGlu: 4.991 ± 0.055
2.641ArgPhe: 2.641 ± 0.04
6.453ArgGly: 6.453 ± 0.064
2.233ArgHis: 2.233 ± 0.033
3.245ArgIle: 3.245 ± 0.038
1.51ArgLys: 1.51 ± 0.03
9.783ArgLeu: 9.783 ± 0.076
1.943ArgMet: 1.943 ± 0.032
1.363ArgAsn: 1.363 ± 0.024
6.044ArgPro: 6.044 ± 0.06
2.095ArgGln: 2.095 ± 0.034
9.092ArgArg: 9.092 ± 0.073
3.987ArgSer: 3.987 ± 0.04
5.425ArgThr: 5.425 ± 0.052
6.929ArgVal: 6.929 ± 0.056
1.452ArgTrp: 1.452 ± 0.024
1.888ArgTyr: 1.888 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.696SerAla: 6.696 ± 0.064
0.378SerCys: 0.378 ± 0.014
2.64SerAsp: 2.64 ± 0.038
2.205SerGlu: 2.205 ± 0.03
1.475SerPhe: 1.475 ± 0.029
6.072SerGly: 6.072 ± 0.069
0.934SerHis: 0.934 ± 0.02
1.256SerIle: 1.256 ± 0.024
0.883SerLys: 0.883 ± 0.023
4.496SerLeu: 4.496 ± 0.049
1.03SerMet: 1.03 ± 0.018
0.768SerAsn: 0.768 ± 0.019
3.281SerPro: 3.281 ± 0.043
1.009SerGln: 1.009 ± 0.026
3.724SerArg: 3.724 ± 0.041
2.638SerSer: 2.638 ± 0.046
2.541SerThr: 2.541 ± 0.038
3.956SerVal: 3.956 ± 0.045
0.837SerTrp: 0.837 ± 0.021
1.074SerTyr: 1.074 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
8.348ThrAla: 8.348 ± 0.066
0.425ThrCys: 0.425 ± 0.017
3.119ThrAsp: 3.119 ± 0.043
2.779ThrGlu: 2.779 ± 0.036
1.68ThrPhe: 1.68 ± 0.025
6.544ThrGly: 6.544 ± 0.063
1.081ThrHis: 1.081 ± 0.024
1.697ThrIle: 1.697 ± 0.029
0.953ThrLys: 0.953 ± 0.025
5.522ThrLeu: 5.522 ± 0.046
0.99ThrMet: 0.99 ± 0.021
0.875ThrAsn: 0.875 ± 0.023
4.027ThrPro: 4.027 ± 0.054
1.073ThrGln: 1.073 ± 0.026
4.194ThrArg: 4.194 ± 0.05
2.95ThrSer: 2.95 ± 0.039
3.262ThrThr: 3.262 ± 0.049
5.807ThrVal: 5.807 ± 0.052
0.878ThrTrp: 0.878 ± 0.02
1.104ThrTyr: 1.104 ± 0.024
0.0ThrXaa: 0.0 ± 0.0
Val
11.816ValAla: 11.816 ± 0.093
0.742ValCys: 0.742 ± 0.018
4.69ValAsp: 4.69 ± 0.044
4.777ValGlu: 4.777 ± 0.05
2.563ValPhe: 2.563 ± 0.038
6.926ValGly: 6.926 ± 0.062
1.935ValHis: 1.935 ± 0.027
2.654ValIle: 2.654 ± 0.035
1.601ValLys: 1.601 ± 0.03
10.063ValLeu: 10.063 ± 0.084
1.448ValMet: 1.448 ± 0.03
1.714ValAsn: 1.714 ± 0.031
5.998ValPro: 5.998 ± 0.052
1.98ValGln: 1.98 ± 0.031
8.148ValArg: 8.148 ± 0.064
4.195ValSer: 4.195 ± 0.049
5.509ValThr: 5.509 ± 0.061
8.466ValVal: 8.466 ± 0.078
1.18ValTrp: 1.18 ± 0.025
1.548ValTyr: 1.548 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.641TrpAla: 1.641 ± 0.03
0.158TrpCys: 0.158 ± 0.009
0.834TrpAsp: 0.834 ± 0.019
0.692TrpGlu: 0.692 ± 0.016
0.515TrpPhe: 0.515 ± 0.015
1.018TrpGly: 1.018 ± 0.022
0.368TrpHis: 0.368 ± 0.013
0.583TrpIle: 0.583 ± 0.017
0.346TrpLys: 0.346 ± 0.014
1.738TrpLeu: 1.738 ± 0.029
0.299TrpMet: 0.299 ± 0.01
0.359TrpAsn: 0.359 ± 0.014
0.838TrpPro: 0.838 ± 0.021
0.435TrpGln: 0.435 ± 0.014
1.526TrpArg: 1.526 ± 0.028
0.837TrpSer: 0.837 ± 0.02
1.157TrpThr: 1.157 ± 0.024
0.956TrpVal: 0.956 ± 0.022
0.362TrpTrp: 0.362 ± 0.014
0.35TrpTyr: 0.35 ± 0.011
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.562TyrAla: 2.562 ± 0.035
0.184TyrCys: 0.184 ± 0.009
1.284TyrAsp: 1.284 ± 0.029
1.095TyrGlu: 1.095 ± 0.024
0.63TyrPhe: 0.63 ± 0.017
2.116TyrGly: 2.116 ± 0.033
0.397TyrHis: 0.397 ± 0.013
0.446TyrIle: 0.446 ± 0.016
0.376TyrLys: 0.376 ± 0.014
2.09TyrLeu: 2.09 ± 0.029
0.254TyrMet: 0.254 ± 0.011
0.364TyrAsn: 0.364 ± 0.014
1.017TyrPro: 1.017 ± 0.024
0.534TyrGln: 0.534 ± 0.016
1.852TyrArg: 1.852 ± 0.029
0.885TyrSer: 0.885 ± 0.021
1.085TyrThr: 1.085 ± 0.024
1.63TyrVal: 1.63 ± 0.029
0.345TyrTrp: 0.345 ± 0.014
0.454TyrTyr: 0.454 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7310 proteins (2210062 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski