Gordonia phage Mollymur
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 113 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6EAC1|A0A4Y6EAC1_9CAUD Phosphoribosyltransferase OS=Gordonia phage Mollymur OX=2590895 GN=5 PE=4 SV=1
MM1 pKa = 7.08 NQGEE5 pKa = 4.44 LLGRR9 pKa = 11.84 ILAGMGYY16 pKa = 6.58 TQHH19 pKa = 7.02 SLDD22 pKa = 4.42 YY23 pKa = 10.72 FDD25 pKa = 5.59 GSCICGWSAEE35 pKa = 4.45 DD36 pKa = 4.85 VPDD39 pKa = 5.62 DD40 pKa = 4.28 PAFDD44 pKa = 4.49 DD45 pKa = 4.85 LSLNEE50 pKa = 4.46 LFEE53 pKa = 4.26 AHH55 pKa = 6.06 VGEE58 pKa = 4.7 AAAA61 pKa = 5.09
Molecular weight: 6.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.528
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.703
Protein with the highest isoelectric point:
>tr|A0A4Y6E9S2|A0A4Y6E9S2_9CAUD Uncharacterized protein OS=Gordonia phage Mollymur OX=2590895 GN=52 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 KK3 pKa = 8.51 HH4 pKa = 5.49 HH5 pKa = 6.17 TRR7 pKa = 11.84 PHH9 pKa = 6.16 AARR12 pKa = 11.84 GRR14 pKa = 11.84 YY15 pKa = 6.83 VTCEE19 pKa = 3.83 CGKK22 pKa = 10.13 RR23 pKa = 11.84 AYY25 pKa = 7.47 EE26 pKa = 3.93 TRR28 pKa = 11.84 KK29 pKa = 9.87 DD30 pKa = 3.51 AKK32 pKa = 10.38 LSRR35 pKa = 11.84 GTMGGSAGTGMAAYY49 pKa = 10.02 RR50 pKa = 11.84 CDD52 pKa = 3.72 LAPASMPWHH61 pKa = 6.74 LGHH64 pKa = 7.33 LNKK67 pKa = 9.94 KK68 pKa = 8.91 SKK70 pKa = 10.35 IYY72 pKa = 10.68 GRR74 pKa = 11.84 LL75 pKa = 3.25
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.809
IPC_protein 10.394
Toseland 10.584
ProMoST 10.233
Dawson 10.716
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 11.082
Grimsley 10.774
Solomon 10.774
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.643
Patrickios 10.818
IPC_peptide 10.789
IPC2_peptide 9.487
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
113
0
113
23199
33
3010
205.3
22.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.526 ± 0.443
0.75 ± 0.126
6.686 ± 0.167
6.599 ± 0.284
2.931 ± 0.153
8.384 ± 0.353
1.918 ± 0.152
5.009 ± 0.183
3.772 ± 0.185
7.91 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.105
3.121 ± 0.171
5.142 ± 0.272
3.582 ± 0.22
7.289 ± 0.241
5.548 ± 0.171
6.005 ± 0.196
6.944 ± 0.197
1.892 ± 0.148
2.634 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here