Tortoise microvirus 22

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6Z2|A0A4P8W6Z2_9VIRU Replication initiation protein OS=Tortoise microvirus 22 OX=2583124 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 5.43IVPHH6 pKa = 5.46STVDD10 pKa = 3.58PDD12 pKa = 3.47DD13 pKa = 3.75SFYY16 pKa = 11.67VDD18 pKa = 3.87FFKK21 pKa = 11.11RR22 pKa = 11.84NLITVVCDD30 pKa = 3.35GDD32 pKa = 3.82NADD35 pKa = 4.13SYY37 pKa = 11.87KK38 pKa = 10.73LALGRR43 pKa = 11.84YY44 pKa = 8.62LLFEE48 pKa = 4.25EE49 pKa = 4.94TFSDD53 pKa = 3.93LASAYY58 pKa = 10.22DD59 pKa = 3.89YY60 pKa = 10.67FVSHH64 pKa = 7.1PVPVFLSLSSILRR77 pKa = 11.84EE78 pKa = 3.85IDD80 pKa = 2.92RR81 pKa = 11.84SAASVPEE88 pKa = 4.05SSSDD92 pKa = 3.47VSEE95 pKa = 4.17

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9C1|A0A4P8W9C1_9VIRU Uncharacterized protein OS=Tortoise microvirus 22 OX=2583124 PE=4 SV=1
MM1 pKa = 7.57SCLNPQYY8 pKa = 10.74RR9 pKa = 11.84KK10 pKa = 9.75NPKK13 pKa = 9.11YY14 pKa = 10.45RR15 pKa = 11.84PNKK18 pKa = 9.61SNGGVPPVCPDD29 pKa = 3.2PRR31 pKa = 11.84LMLLRR36 pKa = 11.84YY37 pKa = 10.03DD38 pKa = 3.71CGKK41 pKa = 10.65CYY43 pKa = 9.91EE44 pKa = 4.34CRR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 9.81RR49 pKa = 11.84ASHH52 pKa = 4.52WRR54 pKa = 11.84FRR56 pKa = 11.84LLQEE60 pKa = 4.22YY61 pKa = 9.85KK62 pKa = 10.59YY63 pKa = 10.9SPTKK67 pKa = 10.23RR68 pKa = 11.84FHH70 pKa = 6.66FVTFTFSEE78 pKa = 4.49DD79 pKa = 3.47SLFSLRR85 pKa = 11.84SEE87 pKa = 5.33FPDD90 pKa = 5.31LDD92 pKa = 4.28DD93 pKa = 3.87NCIAKK98 pKa = 10.07VAVRR102 pKa = 11.84RR103 pKa = 11.84FLEE106 pKa = 4.58RR107 pKa = 11.84YY108 pKa = 7.01RR109 pKa = 11.84KK110 pKa = 9.81RR111 pKa = 11.84YY112 pKa = 8.95GVSLRR117 pKa = 11.84HH118 pKa = 5.79FLVTEE123 pKa = 4.45LGGKK127 pKa = 9.09NGRR130 pKa = 11.84IHH132 pKa = 6.56LHH134 pKa = 6.36GIVMDD139 pKa = 4.9CKK141 pKa = 9.45AGRR144 pKa = 11.84YY145 pKa = 9.15RR146 pKa = 11.84GSKK149 pKa = 10.2YY150 pKa = 10.43IIDD153 pKa = 3.64MDD155 pKa = 4.37LFHH158 pKa = 7.2SIWRR162 pKa = 11.84YY163 pKa = 9.37GYY165 pKa = 10.96CFFGWCNEE173 pKa = 3.6RR174 pKa = 11.84SISYY178 pKa = 5.81VTKK181 pKa = 10.79YY182 pKa = 10.4IMKK185 pKa = 9.23PDD187 pKa = 4.26PYY189 pKa = 10.62HH190 pKa = 5.22PTFRR194 pKa = 11.84PKK196 pKa = 10.7LLLSPGIGSDD206 pKa = 3.53YY207 pKa = 10.82FRR209 pKa = 11.84DD210 pKa = 3.51LSVLRR215 pKa = 11.84FHH217 pKa = 7.4RR218 pKa = 11.84SSSDD222 pKa = 3.47GVWYY226 pKa = 10.24CVSSTGHH233 pKa = 6.6KK234 pKa = 10.43LSMPRR239 pKa = 11.84YY240 pKa = 8.26YY241 pKa = 10.01RR242 pKa = 11.84DD243 pKa = 3.48KK244 pKa = 10.76IFSEE248 pKa = 4.5DD249 pKa = 2.88EE250 pKa = 4.14RR251 pKa = 11.84LRR253 pKa = 11.84HH254 pKa = 6.75SLDD257 pKa = 4.07LLDD260 pKa = 4.7NPPPFVFRR268 pKa = 11.84GRR270 pKa = 11.84EE271 pKa = 3.96FSSFWSFHH279 pKa = 5.37RR280 pKa = 11.84AVYY283 pKa = 10.57AFYY286 pKa = 10.56DD287 pKa = 3.57YY288 pKa = 10.95TLNLGVSDD296 pKa = 4.5ALCAFRR302 pKa = 11.84DD303 pKa = 3.57NLYY306 pKa = 10.02PDD308 pKa = 3.63EE309 pKa = 5.31SFDD312 pKa = 5.11LFDD315 pKa = 5.08PLIEE319 pKa = 4.04SWSQIRR325 pKa = 11.84IFNDD329 pKa = 3.2YY330 pKa = 10.8LCPQVLGYY338 pKa = 10.82

Molecular weight: 40.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1428

95

527

285.6

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.073 ± 2.373

1.401 ± 0.672

7.073 ± 1.068

4.342 ± 0.626

5.112 ± 1.096

5.742 ± 0.741

2.381 ± 0.451

4.412 ± 0.216

3.431 ± 0.435

9.034 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.195

4.412 ± 0.839

4.972 ± 1.179

4.062 ± 1.544

6.513 ± 1.098

11.134 ± 1.537

4.062 ± 0.551

6.793 ± 0.8

1.12 ± 0.249

5.252 ± 0.806
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski