Xylella fastidiosa (strain 9a5c)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9PFC6|Q9PFC6_XYLFA FGE-sulfatase domain-containing protein OS=Xylella fastidiosa (strain 9a5c) OX=160492 GN=XF_0752 PE=4 SV=1
MM1 pKa = 7.79 GIALCLRR8 pKa = 11.84 HH9 pKa = 7.03 ILDD12 pKa = 3.84 SDD14 pKa = 3.53 ITALAVPSSYY24 pKa = 10.06 IAYY27 pKa = 8.93 AVPVYY32 pKa = 10.78 VVDD35 pKa = 5.06 LRR37 pKa = 11.84 PDD39 pKa = 3.19 TSDD42 pKa = 3.55 TNDD45 pKa = 3.07 EE46 pKa = 4.17
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.973
IPC_protein 3.834
Toseland 3.63
ProMoST 3.973
Dawson 3.872
Bjellqvist 4.177
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.554
Solomon 3.834
Lehninger 3.795
Nozaki 4.024
DTASelect 4.291
Thurlkill 3.732
EMBOSS 3.884
Sillero 3.973
Patrickios 0.604
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>sp|Q9PGR0|RS15_XYLFA 30S ribosomal protein S15 OS=Xylella fastidiosa (strain 9a5c) OX=160492 GN=rpsO PE=3 SV=1
MM1 pKa = 7.44 SRR3 pKa = 11.84 PFSVSEE9 pKa = 4.04 TLKK12 pKa = 10.34 ADD14 pKa = 3.37 CFGRR18 pKa = 11.84 ILLVNGKK25 pKa = 8.19 GEE27 pKa = 4.01 KK28 pKa = 9.95 FIRR31 pKa = 11.84 RR32 pKa = 11.84 DD33 pKa = 3.29 LGAVSWWLRR42 pKa = 11.84 GLAGWLARR50 pKa = 11.84 RR51 pKa = 11.84 EE52 pKa = 4.08 VAALRR57 pKa = 11.84 QLNTLPAVPRR67 pKa = 11.84 LLNWDD72 pKa = 4.15 GVCLDD77 pKa = 3.98 RR78 pKa = 11.84 SFLSGQMMYY87 pKa = 10.13 QCPPRR92 pKa = 11.84 GDD94 pKa = 3.32 LAYY97 pKa = 10.59 FRR99 pKa = 11.84 AACRR103 pKa = 11.84 LLQQMHH109 pKa = 6.71 RR110 pKa = 11.84 CGVVHH115 pKa = 7.2 NDD117 pKa = 3.53 LAKK120 pKa = 9.53 EE121 pKa = 4.09 ANWLVLEE128 pKa = 5.1 DD129 pKa = 4.48 GSPGVIDD136 pKa = 3.71 FQLAVRR142 pKa = 11.84 GDD144 pKa = 3.78 PRR146 pKa = 11.84 APWMRR151 pKa = 11.84 LLARR155 pKa = 11.84 EE156 pKa = 4.35 DD157 pKa = 3.42 LRR159 pKa = 11.84 HH160 pKa = 5.47 LLKK163 pKa = 10.4 HH164 pKa = 5.85 KK165 pKa = 10.61 RR166 pKa = 11.84 MYY168 pKa = 10.6 CPEE171 pKa = 4.15 ALTPVEE177 pKa = 4.08 RR178 pKa = 11.84 RR179 pKa = 11.84 LLKK182 pKa = 10.0 RR183 pKa = 11.84 PSWIRR188 pKa = 11.84 RR189 pKa = 11.84 LWFATGKK196 pKa = 9.06 PVYY199 pKa = 10.02 RR200 pKa = 11.84 FVTRR204 pKa = 11.84 RR205 pKa = 11.84 VLHH208 pKa = 6.09 WEE210 pKa = 4.07 DD211 pKa = 3.46 NEE213 pKa = 4.31 GRR215 pKa = 11.84 GAKK218 pKa = 9.25 PP219 pKa = 2.95
Molecular weight: 25.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.472
IPC_protein 10.262
Toseland 10.599
ProMoST 10.306
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.818
Grimsley 10.73
Solomon 10.818
Lehninger 10.789
Nozaki 10.613
DTASelect 10.409
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.643
Patrickios 10.54
IPC_peptide 10.833
IPC2_peptide 9.677
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2772
0
2772
743229
30
3455
268.1
29.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.235 ± 0.061
1.19 ± 0.025
5.3 ± 0.039
5.071 ± 0.054
3.471 ± 0.037
7.586 ± 0.063
2.743 ± 0.032
5.336 ± 0.041
3.562 ± 0.043
10.714 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.028
3.358 ± 0.044
4.926 ± 0.043
4.215 ± 0.035
6.694 ± 0.054
5.98 ± 0.049
5.796 ± 0.085
7.336 ± 0.049
1.464 ± 0.023
2.618 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here