Shigella phage pSb-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Shigella virus Sb1

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5UPK1|V5UPK1_9CAUD Uncharacterized protein OS=Shigella phage pSb-1 OX=1414738 GN=psb1_0028 PE=4 SV=1
MM1 pKa = 7.23NAHH4 pKa = 6.2TPFDD8 pKa = 4.34AKK10 pKa = 10.72QDD12 pKa = 3.5WTNPYY17 pKa = 9.09CQNSSNDD24 pKa = 3.39PMVDD28 pKa = 3.19ALLGNAYY35 pKa = 9.9HH36 pKa = 6.23VVRR39 pKa = 11.84TVYY42 pKa = 10.84CNLGNLKK49 pKa = 10.53LIYY52 pKa = 10.49DD53 pKa = 4.42FLNKK57 pKa = 10.16YY58 pKa = 10.64GMVLGVQSEE67 pKa = 4.95TEE69 pKa = 4.11LKK71 pKa = 10.53AIPTSASYY79 pKa = 10.84VRR81 pKa = 11.84LYY83 pKa = 11.38GFDD86 pKa = 3.48NTNKK90 pKa = 10.13RR91 pKa = 11.84VVTDD95 pKa = 3.3YY96 pKa = 11.33LYY98 pKa = 11.26VEE100 pKa = 4.84GDD102 pKa = 3.35RR103 pKa = 11.84TGVIPDD109 pKa = 4.44DD110 pKa = 4.34PSATGSWILVATSNSDD126 pKa = 3.07SGGDD130 pKa = 3.64DD131 pKa = 3.9GEE133 pKa = 5.02GKK135 pKa = 10.43ASPPYY140 pKa = 10.19IPYY143 pKa = 10.25SYY145 pKa = 11.28NNGSAIGGEE154 pKa = 4.21TTIPVPAGTVGVPMIVIDD172 pKa = 4.94GYY174 pKa = 9.69TNLVGYY180 pKa = 9.87GFTYY184 pKa = 10.5DD185 pKa = 3.94ASTLTVTLAQPLEE198 pKa = 4.64PGDD201 pKa = 3.99EE202 pKa = 4.0VHH204 pKa = 7.05LFLTGTPAVPDD215 pKa = 3.82NPNVSDD221 pKa = 3.47WVQINWLYY229 pKa = 10.72NGGYY233 pKa = 10.39ASGGEE238 pKa = 3.96QVIAIPYY245 pKa = 7.81TFEE248 pKa = 4.11SVPAIYY254 pKa = 10.52KK255 pKa = 10.29NGEE258 pKa = 3.69RR259 pKa = 11.84YY260 pKa = 9.54YY261 pKa = 11.32AGLADD266 pKa = 4.07KK267 pKa = 10.84SYY269 pKa = 11.59AVDD272 pKa = 3.37AANQRR277 pKa = 11.84ILLTEE282 pKa = 4.13PLATNDD288 pKa = 3.85RR289 pKa = 11.84LIIQIGGEE297 pKa = 4.23STTLIMSDD305 pKa = 3.03RR306 pKa = 11.84TVQEE310 pKa = 4.1VARR313 pKa = 11.84SANVHH318 pKa = 5.93EE319 pKa = 4.7NDD321 pKa = 4.83VILSTNTTQYY331 pKa = 11.07LNGMKK336 pKa = 10.2VIYY339 pKa = 10.22DD340 pKa = 3.41VVGQKK345 pKa = 9.86IYY347 pKa = 11.04GLPTLPTNVYY357 pKa = 9.87INSVSNGQLTYY368 pKa = 10.85SPGNITVDD376 pKa = 4.32LLPMPSEE383 pKa = 4.16ALSALEE389 pKa = 3.83TLEE392 pKa = 4.61NSLNSPLGFTNIGRR406 pKa = 11.84VQSFAALRR414 pKa = 11.84TLVPPSAGARR424 pKa = 11.84VLLSGYY430 pKa = 9.98YY431 pKa = 10.39DD432 pKa = 3.53NSDD435 pKa = 3.32TGGGEE440 pKa = 4.45FIARR444 pKa = 11.84STTGMSEE451 pKa = 4.03VPDD454 pKa = 4.37DD455 pKa = 4.72DD456 pKa = 5.41GGVIATVNSNWYY468 pKa = 8.09WEE470 pKa = 4.24RR471 pKa = 11.84VDD473 pKa = 3.73TNNATVEE480 pKa = 4.09DD481 pKa = 5.26FGAISYY487 pKa = 9.23TGDD490 pKa = 3.61DD491 pKa = 3.5TTGIADD497 pKa = 3.95SSDD500 pKa = 2.64AFQRR504 pKa = 11.84MFNSLNRR511 pKa = 11.84IFSADD516 pKa = 2.96SSKK519 pKa = 10.97RR520 pKa = 11.84FVIDD524 pKa = 3.8APILLKK530 pKa = 10.99VPTLKK535 pKa = 10.77LIYY538 pKa = 9.99QGVVV542 pKa = 2.56

Molecular weight: 58.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5UQG1|V5UQG1_9CAUD Uncharacterized protein OS=Shigella phage pSb-1 OX=1414738 GN=psb1_0020 PE=4 SV=1
MM1 pKa = 7.17VRR3 pKa = 11.84RR4 pKa = 11.84LKK6 pKa = 10.73RR7 pKa = 11.84RR8 pKa = 11.84VEE10 pKa = 4.15LVPNWKK16 pKa = 9.95RR17 pKa = 11.84CWKK20 pKa = 8.21WASIQISTFGLIMFSAIDD38 pKa = 4.26IIQPMLTGLPRR49 pKa = 11.84HH50 pKa = 6.0ILEE53 pKa = 5.57DD54 pKa = 3.56IPHH57 pKa = 7.03GSNIAIALFALNIVGRR73 pKa = 11.84LIRR76 pKa = 11.84FRR78 pKa = 11.84PKK80 pKa = 10.29EE81 pKa = 4.06DD82 pKa = 3.18SHH84 pKa = 7.49EE85 pKa = 4.23SS86 pKa = 3.3

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

21918

37

1453

212.8

23.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.491 ± 0.511

0.903 ± 0.119

5.876 ± 0.139

6.314 ± 0.234

3.55 ± 0.142

6.575 ± 0.238

1.752 ± 0.127

5.886 ± 0.18

6.424 ± 0.281

8.258 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.961 ± 0.114

5.717 ± 0.146

4.147 ± 0.141

4.033 ± 0.245

4.581 ± 0.193

6.31 ± 0.209

6.47 ± 0.251

6.912 ± 0.21

1.177 ± 0.093

3.664 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski