Anatid alphaherpesvirus 1
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E6Y398|E6Y398_9ALPH Tegument protein UL47 OS=Anatid alphaherpesvirus 1 OX=104388 GN=UL47 PE=3 SV=1
MM1 pKa = 7.21 QSHH4 pKa = 7.64 PATFITYY11 pKa = 8.53 TLGGTGASHH20 pKa = 6.87 TWTVPEE26 pKa = 4.21 YY27 pKa = 9.7 EE28 pKa = 4.65 QVICSCDD35 pKa = 3.11 GGSRR39 pKa = 11.84 SVLVGNKK46 pKa = 7.29 TRR48 pKa = 11.84 CDD50 pKa = 3.53 KK51 pKa = 11.12 LPPCNVIIQRR61 pKa = 11.84 GPLGTLFVVDD71 pKa = 4.0 IGYY74 pKa = 10.38 AIYY77 pKa = 10.39 SYY79 pKa = 9.58 MLRR82 pKa = 11.84 CDD84 pKa = 4.65 LKK86 pKa = 10.38 QQVGTLSASPGSLYY100 pKa = 10.68 VVPFTSCTVVGVDD113 pKa = 3.26 SYY115 pKa = 11.56 IRR117 pKa = 11.84 SDD119 pKa = 3.19 SSGVLTIAWSHH130 pKa = 4.84 NTVHH134 pKa = 5.87 ITITVYY140 pKa = 10.77 GLSEE144 pKa = 3.94 EE145 pKa = 4.37 SQRR148 pKa = 11.84 MASVSAISTVGQDD161 pKa = 3.51 YY162 pKa = 11.31 EE163 pKa = 4.19 NLQDD167 pKa = 4.32 IANQEE172 pKa = 3.91 QSEE175 pKa = 4.23 EE176 pKa = 4.24 DD177 pKa = 3.86 LLSAAIKK184 pKa = 9.23 EE185 pKa = 4.27 ANIGVDD191 pKa = 3.76 YY192 pKa = 10.98 ISDD195 pKa = 3.98 SEE197 pKa = 4.48 SSSRR201 pKa = 11.84 TVMDD205 pKa = 5.24 DD206 pKa = 3.94 LLTSIQDD213 pKa = 3.58 EE214 pKa = 4.48 CLEE217 pKa = 4.32 TADD220 pKa = 4.59 CFCNGTSQEE229 pKa = 4.23 PIAVNGIDD237 pKa = 3.07 II238 pKa = 4.36
Molecular weight: 25.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.048
IPC2_protein 4.177
IPC_protein 4.139
Toseland 3.948
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.024
Rodwell 3.973
Grimsley 3.859
Solomon 4.113
Lehninger 4.062
Nozaki 4.228
DTASelect 4.431
Thurlkill 3.986
EMBOSS 4.037
Sillero 4.253
Patrickios 0.362
IPC_peptide 4.113
IPC2_peptide 4.24
IPC2.peptide.svr19 4.155
Protein with the highest isoelectric point:
>tr|Q156E1|Q156E1_9ALPH Thymidine kinase OS=Anatid alphaherpesvirus 1 OX=104388 GN=TK PE=3 SV=1
MM1 pKa = 7.01 QLVHH5 pKa = 7.14 HH6 pKa = 7.25 IYY8 pKa = 8.91 STNISIDD15 pKa = 3.59 YY16 pKa = 10.47 IFIICHH22 pKa = 4.99 VDD24 pKa = 3.05 GLVRR28 pKa = 11.84 ARR30 pKa = 11.84 PRR32 pKa = 11.84 PGEE35 pKa = 4.02 ALDD38 pKa = 4.27 PLCHH42 pKa = 6.65 FPGHH46 pKa = 6.1 RR47 pKa = 11.84 KK48 pKa = 9.65 KK49 pKa = 10.98 GAGLFWRR56 pKa = 11.84 GAKK59 pKa = 9.93 RR60 pKa = 11.84 GVAWQHH66 pKa = 6.01 ANSTHH71 pKa = 5.7 WAGLGLLPTPPTISPSHH88 pKa = 5.84 GRR90 pKa = 11.84 GLDD93 pKa = 3.29 HH94 pKa = 6.69 CQPRR98 pKa = 11.84 PWEE101 pKa = 4.27 GRR103 pKa = 11.84 SRR105 pKa = 11.84 PWEE108 pKa = 3.95 EE109 pKa = 3.71 LGLAGFQRR117 pKa = 11.84 FYY119 pKa = 11.09 GIRR122 pKa = 11.84 VRR124 pKa = 11.84 VGRR127 pKa = 11.84 VPKK130 pKa = 10.12 VLRR133 pKa = 11.84 YY134 pKa = 9.42
Molecular weight: 15.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.765
IPC_protein 10.789
Toseland 10.701
ProMoST 10.613
Dawson 10.818
Bjellqvist 10.628
Wikipedia 11.096
Rodwell 10.862
Grimsley 10.891
Solomon 10.994
Lehninger 10.95
Nozaki 10.716
DTASelect 10.613
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.774
Patrickios 10.599
IPC_peptide 10.994
IPC2_peptide 9.926
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
40471
87
3587
546.9
60.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.149 ± 0.239
2.229 ± 0.102
6.002 ± 0.153
5.379 ± 0.148
3.738 ± 0.201
6.239 ± 0.178
2.288 ± 0.074
5.849 ± 0.174
3.929 ± 0.168
8.796 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.636 ± 0.087
4.171 ± 0.09
5.055 ± 0.226
3.059 ± 0.184
6.773 ± 0.184
8.142 ± 0.274
6.832 ± 0.157
6.227 ± 0.12
1.09 ± 0.066
3.417 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here