Salmonella phage SE1 (in:Nonagvirus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Nonagvirus; Salmonella virus SE1Kor

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JS57|A0A1W6JS57_9CAUD Uncharacterized protein OS=Salmonella phage SE1 (in:Nonagvirus) OX=2115848 PE=4 SV=1
MM1 pKa = 6.8EE2 pKa = 5.7TIVLHH7 pKa = 6.04CWVDD11 pKa = 3.73LDD13 pKa = 3.64EE14 pKa = 6.62HH15 pKa = 6.58EE16 pKa = 4.49YY17 pKa = 10.79CVNINNGPVPDD28 pKa = 3.93VTFTSYY34 pKa = 11.74DD35 pKa = 3.33EE36 pKa = 3.65MDD38 pKa = 3.87AYY40 pKa = 11.45VKK42 pKa = 10.46GFRR45 pKa = 11.84EE46 pKa = 4.37CARR49 pKa = 11.84IANINVTVILPEE61 pKa = 3.82

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JS65|A0A1W6JS65_9CAUD Uncharacterized protein OS=Salmonella phage SE1 (in:Nonagvirus) OX=2115848 PE=4 SV=1
MM1 pKa = 7.32QKK3 pKa = 9.51HH4 pKa = 4.17QPLYY8 pKa = 9.88KK9 pKa = 9.88VRR11 pKa = 11.84RR12 pKa = 11.84LGGGLYY18 pKa = 10.3AVEE21 pKa = 4.05RR22 pKa = 11.84NKK24 pKa = 10.74RR25 pKa = 11.84SITIVSVKK33 pKa = 9.8VGSRR37 pKa = 11.84KK38 pKa = 9.39FYY40 pKa = 10.85SVPNCSPLLPTLRR53 pKa = 11.84DD54 pKa = 3.11AVLYY58 pKa = 10.52VLTGTFFF65 pKa = 4.14

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

18496

29

879

198.9

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.915 ± 0.459

1.481 ± 0.158

5.655 ± 0.195

6.774 ± 0.247

3.612 ± 0.159

7.142 ± 0.242

2.006 ± 0.169

6.163 ± 0.203

6.088 ± 0.267

7.515 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.15

4.947 ± 0.181

3.931 ± 0.209

4.698 ± 0.37

5.277 ± 0.215

5.99 ± 0.237

5.704 ± 0.25

7.002 ± 0.259

1.465 ± 0.122

3.66 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski