Euphorbia yellow leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7ENF3|A0A0A7ENF3_9GEMI AC4 OS=Euphorbia yellow leaf curl virus OX=1578825 GN=AC4 PE=3 SV=1
MM1 pKa = 7.99WDD3 pKa = 3.38PLVNEE8 pKa = 4.72FPEE11 pKa = 4.61TVHH14 pKa = 7.0GFRR17 pKa = 11.84CMLAVKK23 pKa = 9.74YY24 pKa = 9.79LQLVADD30 pKa = 4.98TYY32 pKa = 11.79SPDD35 pKa = 3.29TVGYY39 pKa = 10.56DD40 pKa = 3.97LIRR43 pKa = 11.84DD44 pKa = 4.54LICILRR50 pKa = 11.84SRR52 pKa = 11.84NYY54 pKa = 9.76VEE56 pKa = 3.87AQSRR60 pKa = 11.84YY61 pKa = 6.88NHH63 pKa = 6.07FHH65 pKa = 6.58PRR67 pKa = 11.84LEE69 pKa = 4.34GSTEE73 pKa = 3.8AEE75 pKa = 3.9LRR77 pKa = 11.84QSRR80 pKa = 11.84IEE82 pKa = 4.03PCLCPYY88 pKa = 10.25CPRR91 pKa = 11.84HH92 pKa = 5.66QIKK95 pKa = 11.01SMGQQTHH102 pKa = 4.95EE103 pKa = 4.3QKK105 pKa = 11.01AQDD108 pKa = 3.66VQDD111 pKa = 4.0VQKK114 pKa = 10.94SRR116 pKa = 11.84CPP118 pKa = 3.38

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7EQ35|A0A0A7EQ35_9GEMI Capsid protein OS=Euphorbia yellow leaf curl virus OX=1578825 GN=AV1 PE=3 SV=1
MM1 pKa = 7.59GNLIFTCSSSSKK13 pKa = 10.16GSTSARR19 pKa = 11.84ITDD22 pKa = 3.24SSTWYY27 pKa = 8.11PQPGQHH33 pKa = 5.96ISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.54LNQARR46 pKa = 11.84TSSPIWRR53 pKa = 11.84RR54 pKa = 11.84TEE56 pKa = 3.6IPSNGEE62 pKa = 3.99SFRR65 pKa = 11.84STDD68 pKa = 3.6DD69 pKa = 3.48LLEE72 pKa = 4.32EE73 pKa = 4.38VNRR76 pKa = 11.84RR77 pKa = 11.84PTTLTPQRR85 pKa = 11.84LTQAVSQRR93 pKa = 11.84LSEE96 pKa = 4.29SLRR99 pKa = 11.84SS100 pKa = 3.57

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1093

100

350

182.2

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.215 ± 0.572

2.104 ± 0.376

4.666 ± 0.267

4.941 ± 0.75

4.026 ± 0.342

4.758 ± 0.474

4.117 ± 0.91

4.941 ± 0.738

5.672 ± 0.836

7.319 ± 0.9

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.475

5.581 ± 0.666

5.855 ± 0.46

5.032 ± 0.594

7.228 ± 1.247

8.875 ± 1.122

5.672 ± 0.588

6.496 ± 1.215

1.555 ± 0.133

3.934 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski