Thalassococcus sp. S3
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4726 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6MEA2|A0A4P6MEA2_9RHOB N-methyl-l-tryptophan oxidase OS=Thalassococcus sp. S3 OX=2017482 GN=CFI11_09485 PE=4 SV=1
MM1 pKa = 7.1 SHH3 pKa = 6.71 SPEE6 pKa = 4.26 PLEE9 pKa = 4.2 GAPLIAPSSTDD20 pKa = 2.96 HH21 pKa = 6.96 PLYY24 pKa = 10.12 EE25 pKa = 4.26 QVVDD29 pKa = 3.94 ACRR32 pKa = 11.84 TVYY35 pKa = 10.75 DD36 pKa = 3.83 PEE38 pKa = 5.09 IPVNIYY44 pKa = 10.52 EE45 pKa = 4.61 LGLVYY50 pKa = 10.33 TIDD53 pKa = 3.38 INPEE57 pKa = 3.8 NEE59 pKa = 4.1 VKK61 pKa = 10.84 VIMTLTAPGCPVAGEE76 pKa = 4.15 MPGWVADD83 pKa = 4.8 AIEE86 pKa = 4.61 PLPGVKK92 pKa = 9.77 QVDD95 pKa = 3.28 VDD97 pKa = 4.39 LTWEE101 pKa = 4.65 PPWGMDD107 pKa = 3.35 MMSDD111 pKa = 3.68 EE112 pKa = 4.89 ARR114 pKa = 11.84 LEE116 pKa = 4.14 LGFMM120 pKa = 4.54
Molecular weight: 13.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.834
IPC_protein 3.757
Toseland 3.567
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.617
EMBOSS 3.656
Sillero 3.872
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A4P6MEW6|A0A4P6MEW6_9RHOB ABC transporter substrate-binding protein OS=Thalassococcus sp. S3 OX=2017482 GN=CFI11_07560 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4726
0
4726
1495523
32
2520
316.4
34.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.032 ± 0.049
0.88 ± 0.012
6.345 ± 0.042
5.895 ± 0.032
3.787 ± 0.021
8.57 ± 0.046
2.075 ± 0.017
5.173 ± 0.028
2.949 ± 0.025
10.137 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.019
2.553 ± 0.021
5.117 ± 0.027
3.318 ± 0.021
6.769 ± 0.042
5.259 ± 0.026
5.564 ± 0.023
7.17 ± 0.03
1.419 ± 0.014
2.241 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here