Escherichia phage vB_EcoS_HSE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455I0J5|A0A455I0J5_9CAUD Terminase small subunit OS=Escherichia phage vB_EcoS_HSE2 OX=2053690 GN=HSE2_gp030 PE=4 SV=1
MM1 pKa = 6.36VQRR4 pKa = 11.84YY5 pKa = 6.84NASYY9 pKa = 10.73NDD11 pKa = 3.97YY12 pKa = 10.36YY13 pKa = 11.24EE14 pKa = 4.56NDD16 pKa = 3.23AGEE19 pKa = 4.25FVSYY23 pKa = 10.84EE24 pKa = 4.07DD25 pKa = 3.99YY26 pKa = 11.58AKK28 pKa = 11.13LEE30 pKa = 4.39ADD32 pKa = 4.74FKK34 pKa = 11.11AYY36 pKa = 9.47KK37 pKa = 10.29ASAYY41 pKa = 9.14MVAEE45 pKa = 4.3QYY47 pKa = 11.41AGVIKK52 pKa = 10.29EE53 pKa = 4.8LEE55 pKa = 4.34AEE57 pKa = 4.07LQKK60 pKa = 11.45YY61 pKa = 9.63KK62 pKa = 10.98DD63 pKa = 3.48QFPDD67 pKa = 3.4YY68 pKa = 10.69VEE70 pKa = 4.48CANCGSVTHH79 pKa = 5.78VEE81 pKa = 4.11EE82 pKa = 4.74VEE84 pKa = 3.98

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455I3I4|A0A455I3I4_9CAUD Putative structural protein OS=Escherichia phage vB_EcoS_HSE2 OX=2053690 GN=HSE2_gp032 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.35ILKK9 pKa = 10.63RR10 pKa = 11.84SGLAPLSPKK19 pKa = 10.36VKK21 pKa = 9.41TRR23 pKa = 11.84ARR25 pKa = 11.84NATYY29 pKa = 10.89NEE31 pKa = 3.68LAEE34 pKa = 4.0RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 10.07AVRR40 pKa = 11.84ACGFQNGKK48 pKa = 9.72AVNLGEE54 pKa = 4.7FKK56 pKa = 9.58TQEE59 pKa = 3.57RR60 pKa = 11.84AAIANRR66 pKa = 11.84LFNYY70 pKa = 7.62WKK72 pKa = 10.65SLGYY76 pKa = 10.66DD77 pKa = 4.56DD78 pKa = 6.25IPTKK82 pKa = 8.88PQRR85 pKa = 11.84RR86 pKa = 11.84QYY88 pKa = 9.93IWRR91 pKa = 11.84HH92 pKa = 4.21KK93 pKa = 10.55

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11649

67

778

211.8

23.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.37 ± 0.506

1.167 ± 0.157

5.743 ± 0.285

6.455 ± 0.485

3.674 ± 0.171

7.529 ± 0.308

1.717 ± 0.221

5.091 ± 0.148

5.872 ± 0.392

7.855 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.122

4.103 ± 0.282

3.94 ± 0.268

3.88 ± 0.343

5.528 ± 0.312

6.232 ± 0.337

6.49 ± 0.398

7.159 ± 0.304

1.416 ± 0.177

3.331 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski