Microbacterium sp. SYP-A9085

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I2FMK3|A0A6I2FMK3_9MICO Uncharacterized protein OS=Microbacterium sp. SYP-A9085 OX=2664454 GN=GH740_08210 PE=4 SV=1
MM1 pKa = 7.52TYY3 pKa = 10.59VIALPCVDD11 pKa = 3.79VKK13 pKa = 11.28DD14 pKa = 3.96RR15 pKa = 11.84ACVDD19 pKa = 3.71EE20 pKa = 5.5CPVDD24 pKa = 4.37CIYY27 pKa = 11.14EE28 pKa = 4.29GDD30 pKa = 3.56RR31 pKa = 11.84SLYY34 pKa = 9.55IHH36 pKa = 7.48PDD38 pKa = 3.01EE39 pKa = 5.64CVDD42 pKa = 4.38CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.4AIYY56 pKa = 11.22YY57 pKa = 10.15EE58 pKa = 4.88DD59 pKa = 5.06DD60 pKa = 5.26LPEE63 pKa = 3.97EE64 pKa = 3.77WADD67 pKa = 3.65YY68 pKa = 10.7YY69 pKa = 11.19KK70 pKa = 11.41VNVEE74 pKa = 4.29FFDD77 pKa = 5.18DD78 pKa = 3.91LGSPGGAAKK87 pKa = 10.6VGVIHH92 pKa = 7.27KK93 pKa = 8.46DD94 pKa = 3.4HH95 pKa = 7.42PIVAALPPQGAA106 pKa = 3.55

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I2FBF6|A0A6I2FBF6_9MICO Uncharacterized protein OS=Microbacterium sp. SYP-A9085 OX=2664454 GN=GH740_00955 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2676

0

2676

890392

31

1987

332.7

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.226 ± 0.072

0.542 ± 0.01

6.347 ± 0.039

4.944 ± 0.043

3.027 ± 0.024

9.019 ± 0.036

2.113 ± 0.025

4.479 ± 0.033

1.896 ± 0.032

9.888 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.886 ± 0.017

1.775 ± 0.024

5.475 ± 0.035

2.907 ± 0.027

7.785 ± 0.05

5.051 ± 0.035

6.068 ± 0.043

9.15 ± 0.041

1.459 ± 0.018

1.962 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski