Simonsiella muelleri ATCC 29453

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Simonsiella; Simonsiella muelleri

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9HL40|V9HL40_9NEIS 50S ribosomal protein L32 OS=Simonsiella muelleri ATCC 29453 OX=641147 GN=rpmF PE=3 SV=1
MM1 pKa = 7.63SDD3 pKa = 3.37EE4 pKa = 4.35SPIIFTDD11 pKa = 3.95ACCVKK16 pKa = 10.51VADD19 pKa = 6.37LIAEE23 pKa = 4.22EE24 pKa = 4.37NNPNLMLRR32 pKa = 11.84VFVNGGGCSGFQYY45 pKa = 10.94GFTFDD50 pKa = 4.51EE51 pKa = 4.93IKK53 pKa = 10.94NDD55 pKa = 3.76DD56 pKa = 3.83DD57 pKa = 6.13FEE59 pKa = 4.89IEE61 pKa = 4.17KK62 pKa = 10.95NGLKK66 pKa = 10.53FLVDD70 pKa = 3.66PMSYY74 pKa = 10.19QYY76 pKa = 11.57LVGAEE81 pKa = 3.68IDD83 pKa = 3.91YY84 pKa = 10.5TEE86 pKa = 4.57SLQGSQFVIRR96 pKa = 11.84NPNAEE101 pKa = 4.36TTCGCGSSFSVV112 pKa = 3.54

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9H5H6|V9H5H6_9NEIS 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Simonsiella muelleri ATCC 29453 OX=641147 GN=fabZ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2220

0

2220

661657

20

2313

298.0

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.138 ± 0.07

0.97 ± 0.021

5.241 ± 0.039

5.886 ± 0.053

4.173 ± 0.042

6.543 ± 0.05

2.323 ± 0.029

6.398 ± 0.04

5.781 ± 0.051

10.023 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.026

5.141 ± 0.051

3.931 ± 0.036

5.123 ± 0.045

4.609 ± 0.037

5.707 ± 0.038

5.454 ± 0.053

6.589 ± 0.054

1.259 ± 0.023

3.086 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski