Amino acid dipepetide frequency for Agromyces sp. Leaf222

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.171AlaAla: 22.171 ± 0.208
0.657AlaCys: 0.657 ± 0.025
8.998AlaAsp: 8.998 ± 0.104
8.604AlaGlu: 8.604 ± 0.101
4.334AlaPhe: 4.334 ± 0.069
12.756AlaGly: 12.756 ± 0.13
2.366AlaHis: 2.366 ± 0.045
6.238AlaIle: 6.238 ± 0.087
2.734AlaLys: 2.734 ± 0.061
13.58AlaLeu: 13.58 ± 0.115
2.755AlaMet: 2.755 ± 0.046
2.571AlaAsn: 2.571 ± 0.052
6.937AlaPro: 6.937 ± 0.1
3.369AlaGln: 3.369 ± 0.059
9.03AlaArg: 9.03 ± 0.113
8.684AlaSer: 8.684 ± 0.089
8.013AlaThr: 8.013 ± 0.112
11.932AlaVal: 11.932 ± 0.123
2.044AlaTrp: 2.044 ± 0.043
2.523AlaTyr: 2.523 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.564CysAla: 0.564 ± 0.02
0.047CysCys: 0.047 ± 0.006
0.3CysAsp: 0.3 ± 0.016
0.258CysGlu: 0.258 ± 0.017
0.158CysPhe: 0.158 ± 0.012
0.541CysGly: 0.541 ± 0.025
0.101CysHis: 0.101 ± 0.008
0.203CysIle: 0.203 ± 0.012
0.056CysLys: 0.056 ± 0.007
0.41CysLeu: 0.41 ± 0.018
0.076CysMet: 0.076 ± 0.007
0.101CysAsn: 0.101 ± 0.009
0.28CysPro: 0.28 ± 0.019
0.094CysGln: 0.094 ± 0.009
0.295CysArg: 0.295 ± 0.016
0.32CysSer: 0.32 ± 0.017
0.316CysThr: 0.316 ± 0.02
0.388CysVal: 0.388 ± 0.017
0.078CysTrp: 0.078 ± 0.009
0.089CysTyr: 0.089 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
9.884AspAla: 9.884 ± 0.111
0.242AspCys: 0.242 ± 0.016
4.619AspAsp: 4.619 ± 0.076
4.75AspGlu: 4.75 ± 0.068
1.652AspPhe: 1.652 ± 0.033
6.605AspGly: 6.605 ± 0.094
1.179AspHis: 1.179 ± 0.033
2.043AspIle: 2.043 ± 0.044
0.787AspLys: 0.787 ± 0.029
6.599AspLeu: 6.599 ± 0.069
0.702AspMet: 0.702 ± 0.024
0.836AspAsn: 0.836 ± 0.03
4.363AspPro: 4.363 ± 0.069
1.426AspGln: 1.426 ± 0.041
4.75AspArg: 4.75 ± 0.069
2.459AspSer: 2.459 ± 0.048
2.652AspThr: 2.652 ± 0.055
5.592AspVal: 5.592 ± 0.078
0.999AspTrp: 0.999 ± 0.031
1.25AspTyr: 1.25 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
7.771GluAla: 7.771 ± 0.091
0.243GluCys: 0.243 ± 0.015
2.214GluAsp: 2.214 ± 0.049
2.606GluGlu: 2.606 ± 0.052
1.96GluPhe: 1.96 ± 0.043
4.088GluGly: 4.088 ± 0.071
1.685GluHis: 1.685 ± 0.038
2.627GluIle: 2.627 ± 0.044
1.054GluLys: 1.054 ± 0.037
7.02GluLeu: 7.02 ± 0.085
0.855GluMet: 0.855 ± 0.031
1.185GluAsn: 1.185 ± 0.033
3.454GluPro: 3.454 ± 0.061
2.312GluGln: 2.312 ± 0.05
5.702GluArg: 5.702 ± 0.082
2.968GluSer: 2.968 ± 0.058
3.008GluThr: 3.008 ± 0.062
5.022GluVal: 5.022 ± 0.068
0.967GluTrp: 0.967 ± 0.027
1.231GluTyr: 1.231 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.562PheAla: 4.562 ± 0.054
0.156PheCys: 0.156 ± 0.012
2.553PheAsp: 2.553 ± 0.048
1.973PheGlu: 1.973 ± 0.037
1.001PhePhe: 1.001 ± 0.032
3.57PheGly: 3.57 ± 0.066
0.553PheHis: 0.553 ± 0.024
1.263PheIle: 1.263 ± 0.038
0.431PheLys: 0.431 ± 0.018
2.708PheLeu: 2.708 ± 0.051
0.425PheMet: 0.425 ± 0.02
0.652PheAsn: 0.652 ± 0.023
1.386PhePro: 1.386 ± 0.032
0.728PheGln: 0.728 ± 0.025
1.818PheArg: 1.818 ± 0.044
1.688PheSer: 1.688 ± 0.037
2.228PheThr: 2.228 ± 0.052
2.896PheVal: 2.896 ± 0.056
0.542PheTrp: 0.542 ± 0.027
0.623PheTyr: 0.623 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
11.673GlyAla: 11.673 ± 0.135
0.552GlyCys: 0.552 ± 0.022
5.45GlyAsp: 5.45 ± 0.081
5.23GlyGlu: 5.23 ± 0.067
3.412GlyPhe: 3.412 ± 0.064
8.102GlyGly: 8.102 ± 0.109
1.762GlyHis: 1.762 ± 0.039
4.679GlyIle: 4.679 ± 0.061
1.826GlyLys: 1.826 ± 0.049
9.079GlyLeu: 9.079 ± 0.106
1.813GlyMet: 1.813 ± 0.038
1.663GlyAsn: 1.663 ± 0.045
3.843GlyPro: 3.843 ± 0.06
2.124GlyGln: 2.124 ± 0.046
6.584GlyArg: 6.584 ± 0.081
5.56GlySer: 5.56 ± 0.074
5.76GlyThr: 5.76 ± 0.081
8.119GlyVal: 8.119 ± 0.093
1.774GlyTrp: 1.774 ± 0.041
2.207GlyTyr: 2.207 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.376HisAla: 2.376 ± 0.054
0.102HisCys: 0.102 ± 0.009
1.398HisAsp: 1.398 ± 0.037
1.249HisGlu: 1.249 ± 0.033
0.556HisPhe: 0.556 ± 0.023
2.023HisGly: 2.023 ± 0.043
0.512HisHis: 0.512 ± 0.023
0.602HisIle: 0.602 ± 0.025
0.233HisLys: 0.233 ± 0.013
2.027HisLeu: 2.027 ± 0.044
0.272HisMet: 0.272 ± 0.016
0.308HisAsn: 0.308 ± 0.015
1.463HisPro: 1.463 ± 0.035
0.433HisGln: 0.433 ± 0.018
1.558HisArg: 1.558 ± 0.035
0.87HisSer: 0.87 ± 0.028
0.899HisThr: 0.899 ± 0.027
1.675HisVal: 1.675 ± 0.035
0.295HisTrp: 0.295 ± 0.017
0.396HisTyr: 0.396 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.319IleAla: 7.319 ± 0.089
0.216IleCys: 0.216 ± 0.012
3.595IleAsp: 3.595 ± 0.053
2.993IleGlu: 2.993 ± 0.053
1.067IlePhe: 1.067 ± 0.033
4.741IleGly: 4.741 ± 0.069
0.619IleHis: 0.619 ± 0.024
1.705IleIle: 1.705 ± 0.046
0.665IleLys: 0.665 ± 0.029
3.243IleLeu: 3.243 ± 0.057
0.591IleMet: 0.591 ± 0.022
0.73IleAsn: 0.73 ± 0.03
2.195IlePro: 2.195 ± 0.042
0.817IleGln: 0.817 ± 0.029
2.769IleArg: 2.769 ± 0.048
2.24IleSer: 2.24 ± 0.043
2.73IleThr: 2.73 ± 0.045
4.634IleVal: 4.634 ± 0.074
0.542IleTrp: 0.542 ± 0.022
0.686IleTyr: 0.686 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
2.225LysAla: 2.225 ± 0.055
0.055LysCys: 0.055 ± 0.008
0.874LysAsp: 0.874 ± 0.033
0.769LysGlu: 0.769 ± 0.027
0.467LysPhe: 0.467 ± 0.02
1.489LysGly: 1.489 ± 0.042
0.401LysHis: 0.401 ± 0.02
0.752LysIle: 0.752 ± 0.027
0.556LysLys: 0.556 ± 0.033
1.851LysLeu: 1.851 ± 0.042
0.28LysMet: 0.28 ± 0.014
0.453LysAsn: 0.453 ± 0.021
1.153LysPro: 1.153 ± 0.037
0.646LysGln: 0.646 ± 0.022
1.426LysArg: 1.426 ± 0.037
1.045LysSer: 1.045 ± 0.031
1.156LysThr: 1.156 ± 0.036
1.632LysVal: 1.632 ± 0.038
0.243LysTrp: 0.243 ± 0.014
0.408LysTyr: 0.408 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
15.37LeuAla: 15.37 ± 0.141
0.437LeuCys: 0.437 ± 0.018
7.131LeuAsp: 7.131 ± 0.089
5.808LeuGlu: 5.808 ± 0.066
2.73LeuPhe: 2.73 ± 0.06
9.461LeuGly: 9.461 ± 0.103
1.774LeuHis: 1.774 ± 0.04
4.203LeuIle: 4.203 ± 0.071
1.632LeuLys: 1.632 ± 0.045
9.955LeuLeu: 9.955 ± 0.134
1.467LeuMet: 1.467 ± 0.036
1.714LeuAsn: 1.714 ± 0.044
5.388LeuPro: 5.388 ± 0.072
2.31LeuGln: 2.31 ± 0.045
6.83LeuArg: 6.83 ± 0.092
5.37LeuSer: 5.37 ± 0.07
5.779LeuThr: 5.779 ± 0.074
10.105LeuVal: 10.105 ± 0.135
1.235LeuTrp: 1.235 ± 0.036
1.514LeuTyr: 1.514 ± 0.037
0.002LeuXaa: 0.002 ± 0.001
Met
1.952MetAla: 1.952 ± 0.04
0.079MetCys: 0.079 ± 0.008
0.704MetAsp: 0.704 ± 0.025
0.523MetGlu: 0.523 ± 0.022
0.516MetPhe: 0.516 ± 0.022
1.161MetGly: 1.161 ± 0.036
0.364MetHis: 0.364 ± 0.015
0.799MetIle: 0.799 ± 0.026
0.337MetLys: 0.337 ± 0.016
1.938MetLeu: 1.938 ± 0.042
0.267MetMet: 0.267 ± 0.016
0.472MetAsn: 0.472 ± 0.018
1.24MetPro: 1.24 ± 0.03
0.567MetGln: 0.567 ± 0.02
1.331MetArg: 1.331 ± 0.03
1.351MetSer: 1.351 ± 0.035
1.554MetThr: 1.554 ± 0.036
1.193MetVal: 1.193 ± 0.033
0.187MetTrp: 0.187 ± 0.014
0.271MetTyr: 0.271 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.482AsnAla: 2.482 ± 0.049
0.111AsnCys: 0.111 ± 0.009
1.177AsnAsp: 1.177 ± 0.039
1.006AsnGlu: 1.006 ± 0.03
0.614AsnPhe: 0.614 ± 0.022
2.02AsnGly: 2.02 ± 0.05
0.358AsnHis: 0.358 ± 0.02
0.735AsnIle: 0.735 ± 0.024
0.3AsnLys: 0.3 ± 0.018
1.867AsnLeu: 1.867 ± 0.039
0.307AsnMet: 0.307 ± 0.016
0.416AsnAsn: 0.416 ± 0.021
1.551AsnPro: 1.551 ± 0.042
0.536AsnGln: 0.536 ± 0.022
1.286AsnArg: 1.286 ± 0.032
0.92AsnSer: 0.92 ± 0.028
1.09AsnThr: 1.09 ± 0.039
1.656AsnVal: 1.656 ± 0.04
0.291AsnTrp: 0.291 ± 0.016
0.464AsnTyr: 0.464 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.446ProAla: 7.446 ± 0.088
0.174ProCys: 0.174 ± 0.012
4.0ProAsp: 4.0 ± 0.064
3.947ProGlu: 3.947 ± 0.065
1.77ProPhe: 1.77 ± 0.036
5.141ProGly: 5.141 ± 0.07
1.0ProHis: 1.0 ± 0.029
2.3ProIle: 2.3 ± 0.044
1.078ProLys: 1.078 ± 0.035
4.709ProLeu: 4.709 ± 0.072
0.867ProMet: 0.867 ± 0.028
1.137ProAsn: 1.137 ± 0.033
2.28ProPro: 2.28 ± 0.06
1.302ProGln: 1.302 ± 0.034
3.203ProArg: 3.203 ± 0.055
3.283ProSer: 3.283 ± 0.055
3.551ProThr: 3.551 ± 0.071
5.171ProVal: 5.171 ± 0.074
0.891ProTrp: 0.891 ± 0.028
1.082ProTyr: 1.082 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.473GlnAla: 3.473 ± 0.061
0.09GlnCys: 0.09 ± 0.009
1.036GlnAsp: 1.036 ± 0.028
1.056GlnGlu: 1.056 ± 0.033
0.851GlnPhe: 0.851 ± 0.025
1.922GlnGly: 1.922 ± 0.042
0.555GlnHis: 0.555 ± 0.021
1.141GlnIle: 1.141 ± 0.033
0.548GlnLys: 0.548 ± 0.022
2.82GlnLeu: 2.82 ± 0.045
0.438GlnMet: 0.438 ± 0.022
0.531GlnAsn: 0.531 ± 0.019
1.485GlnPro: 1.485 ± 0.034
1.017GlnGln: 1.017 ± 0.034
2.198GlnArg: 2.198 ± 0.05
1.319GlnSer: 1.319 ± 0.034
1.395GlnThr: 1.395 ± 0.036
2.382GlnVal: 2.382 ± 0.046
0.425GlnTrp: 0.425 ± 0.021
0.593GlnTyr: 0.593 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
8.875ArgAla: 8.875 ± 0.1
0.269ArgCys: 0.269 ± 0.018
4.247ArgAsp: 4.247 ± 0.058
4.233ArgGlu: 4.233 ± 0.067
2.482ArgPhe: 2.482 ± 0.045
5.402ArgGly: 5.402 ± 0.079
1.6ArgHis: 1.6 ± 0.039
3.625ArgIle: 3.625 ± 0.053
1.164ArgLys: 1.164 ± 0.037
7.536ArgLeu: 7.536 ± 0.108
1.69ArgMet: 1.69 ± 0.039
1.322ArgAsn: 1.322 ± 0.031
3.653ArgPro: 3.653 ± 0.056
1.756ArgGln: 1.756 ± 0.04
6.672ArgArg: 6.672 ± 0.094
4.131ArgSer: 4.131 ± 0.062
4.13ArgThr: 4.13 ± 0.059
6.112ArgVal: 6.112 ± 0.073
1.141ArgTrp: 1.141 ± 0.033
1.545ArgTyr: 1.545 ± 0.041
0.002ArgXaa: 0.002 ± 0.001
Ser
7.47SerAla: 7.47 ± 0.098
0.271SerCys: 0.271 ± 0.016
3.139SerAsp: 3.139 ± 0.047
2.703SerGlu: 2.703 ± 0.054
1.934SerPhe: 1.934 ± 0.044
5.971SerGly: 5.971 ± 0.081
0.955SerHis: 0.955 ± 0.029
2.69SerIle: 2.69 ± 0.05
1.13SerLys: 1.13 ± 0.035
5.26SerLeu: 5.26 ± 0.077
1.138SerMet: 1.138 ± 0.03
1.094SerAsn: 1.094 ± 0.033
3.144SerPro: 3.144 ± 0.045
1.25SerGln: 1.25 ± 0.033
3.624SerArg: 3.624 ± 0.055
3.256SerSer: 3.256 ± 0.058
3.808SerThr: 3.808 ± 0.07
4.917SerVal: 4.917 ± 0.068
0.914SerTrp: 0.914 ± 0.027
1.161SerTyr: 1.161 ± 0.035
0.001SerXaa: 0.001 ± 0.001
Thr
7.915ThrAla: 7.915 ± 0.081
0.292ThrCys: 0.292 ± 0.018
3.748ThrAsp: 3.748 ± 0.055
3.111ThrGlu: 3.111 ± 0.059
1.86ThrPhe: 1.86 ± 0.048
5.924ThrGly: 5.924 ± 0.089
1.031ThrHis: 1.031 ± 0.03
2.872ThrIle: 2.872 ± 0.062
1.208ThrLys: 1.208 ± 0.035
5.754ThrLeu: 5.754 ± 0.069
0.949ThrMet: 0.949 ± 0.028
1.236ThrAsn: 1.236 ± 0.039
3.796ThrPro: 3.796 ± 0.071
1.296ThrGln: 1.296 ± 0.036
3.528ThrArg: 3.528 ± 0.058
3.491ThrSer: 3.491 ± 0.067
4.034ThrThr: 4.034 ± 0.07
5.774ThrVal: 5.774 ± 0.093
1.002ThrTrp: 1.002 ± 0.032
1.129ThrTyr: 1.129 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
12.553ValAla: 12.553 ± 0.135
0.477ValCys: 0.477 ± 0.021
6.11ValAsp: 6.11 ± 0.075
5.368ValGlu: 5.368 ± 0.079
3.091ValPhe: 3.091 ± 0.054
7.318ValGly: 7.318 ± 0.096
1.773ValHis: 1.773 ± 0.04
4.252ValIle: 4.252 ± 0.062
1.545ValLys: 1.545 ± 0.038
9.778ValLeu: 9.778 ± 0.101
1.343ValMet: 1.343 ± 0.035
1.814ValAsn: 1.814 ± 0.043
5.01ValPro: 5.01 ± 0.062
2.229ValGln: 2.229 ± 0.039
6.122ValArg: 6.122 ± 0.072
4.855ValSer: 4.855 ± 0.064
5.637ValThr: 5.637 ± 0.101
9.263ValVal: 9.263 ± 0.11
1.178ValTrp: 1.178 ± 0.031
1.557ValTyr: 1.557 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.801TrpAla: 1.801 ± 0.04
0.094TrpCys: 0.094 ± 0.009
0.741TrpAsp: 0.741 ± 0.023
0.635TrpGlu: 0.635 ± 0.021
0.607TrpPhe: 0.607 ± 0.024
1.095TrpGly: 1.095 ± 0.032
0.356TrpHis: 0.356 ± 0.016
0.773TrpIle: 0.773 ± 0.027
0.302TrpLys: 0.302 ± 0.017
1.815TrpLeu: 1.815 ± 0.046
0.33TrpMet: 0.33 ± 0.016
0.524TrpAsn: 0.524 ± 0.021
0.758TrpPro: 0.758 ± 0.026
0.54TrpGln: 0.54 ± 0.022
1.244TrpArg: 1.244 ± 0.032
0.971TrpSer: 0.971 ± 0.029
0.984TrpThr: 0.984 ± 0.029
1.187TrpVal: 1.187 ± 0.035
0.408TrpTrp: 0.408 ± 0.019
0.327TrpTyr: 0.327 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.415TyrAla: 2.415 ± 0.043
0.112TyrCys: 0.112 ± 0.009
1.353TyrAsp: 1.353 ± 0.04
1.148TyrGlu: 1.148 ± 0.034
0.694TyrPhe: 0.694 ± 0.024
1.881TyrGly: 1.881 ± 0.048
0.307TyrHis: 0.307 ± 0.018
0.595TyrIle: 0.595 ± 0.023
0.293TyrLys: 0.293 ± 0.018
2.265TyrLeu: 2.265 ± 0.047
0.243TyrMet: 0.243 ± 0.014
0.431TyrAsn: 0.431 ± 0.021
0.952TyrPro: 0.952 ± 0.031
0.525TyrGln: 0.525 ± 0.024
1.614TyrArg: 1.614 ± 0.038
1.05TyrSer: 1.05 ± 0.032
1.222TyrThr: 1.222 ± 0.033
1.624TyrVal: 1.624 ± 0.034
0.335TyrTrp: 0.335 ± 0.018
0.468TyrTyr: 0.468 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.001XaaGlu: 0.001 ± 0.001
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 3708 proteins (1252788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski