Alistipes sp. CHKCI003
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2504 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143XFJ7|A0A143XFJ7_9BACT Outer membrane lipoprotein Blc OS=Alistipes sp. CHKCI003 OX=1780376 GN=blc PE=3 SV=1
MM1 pKa = 7.44 SIKK4 pKa = 10.55 HH5 pKa = 6.32 LFYY8 pKa = 11.19 ALAVSGMLSGAVSCADD24 pKa = 4.2 DD25 pKa = 3.86 EE26 pKa = 5.04 PEE28 pKa = 4.25 TEE30 pKa = 4.3 VVPVDD35 pKa = 3.67 FTLRR39 pKa = 11.84 LNMPISLTDD48 pKa = 3.7 PKK50 pKa = 10.13 LTSVDD55 pKa = 3.73 CAFTRR60 pKa = 11.84 VEE62 pKa = 4.04 TGDD65 pKa = 3.35 EE66 pKa = 3.95 VRR68 pKa = 11.84 ITEE71 pKa = 4.11 FTEE74 pKa = 4.54 AEE76 pKa = 3.93 TGAYY80 pKa = 8.86 EE81 pKa = 4.34 VVATLEE87 pKa = 4.0 EE88 pKa = 4.29 GTYY91 pKa = 10.53 NIAVSGQIAYY101 pKa = 8.64 TLDD104 pKa = 3.41 GNTAEE109 pKa = 4.78 RR110 pKa = 11.84 DD111 pKa = 3.3 IEE113 pKa = 4.01 ASRR116 pKa = 11.84 EE117 pKa = 4.09 GVAVSAASDD126 pKa = 3.5 VLTIEE131 pKa = 4.29 TTVAAMKK138 pKa = 10.57 SDD140 pKa = 3.48 DD141 pKa = 3.92 FVIEE145 pKa = 4.48 EE146 pKa = 3.91 IFYY149 pKa = 10.05 TGTEE153 pKa = 4.28 TPEE156 pKa = 3.66 GMPYY160 pKa = 10.69 YY161 pKa = 10.36 GDD163 pKa = 3.14 QYY165 pKa = 11.5 MKK167 pKa = 9.5 ITNNSDD173 pKa = 2.88 RR174 pKa = 11.84 TLYY177 pKa = 11.13 ADD179 pKa = 3.48 GLVIFKK185 pKa = 10.47 SAFMTVEE192 pKa = 4.14 KK193 pKa = 10.8 YY194 pKa = 10.79 DD195 pKa = 3.73 YY196 pKa = 10.28 TPDD199 pKa = 3.07 IMSDD203 pKa = 3.73 YY204 pKa = 10.72 FSVDD208 pKa = 2.47 SGLILPGSGTDD219 pKa = 3.54 YY220 pKa = 10.77 PVAPGASVVIADD232 pKa = 3.73 TAIDD236 pKa = 3.5 HH237 pKa = 6.64 TEE239 pKa = 3.98 FNSLSLDD246 pKa = 3.42 LSGATFEE253 pKa = 4.74 YY254 pKa = 10.76 FNTLDD259 pKa = 5.37 DD260 pKa = 4.6 EE261 pKa = 6.0 DD262 pKa = 4.46 MDD264 pKa = 4.78 NPDD267 pKa = 3.64 VPNVTTLFADD277 pKa = 5.79 DD278 pKa = 3.76 FWTWHH283 pKa = 5.07 NRR285 pKa = 11.84 GFYY288 pKa = 9.51 TYY290 pKa = 10.71 AIGRR294 pKa = 11.84 LGEE297 pKa = 4.24 GVTAEE302 pKa = 4.57 SFASDD307 pKa = 3.38 YY308 pKa = 10.95 AYY310 pKa = 9.23 TVEE313 pKa = 4.21 YY314 pKa = 10.75 EE315 pKa = 4.39 MVVPDD320 pKa = 4.02 YY321 pKa = 10.53 GTFPMSDD328 pKa = 4.6 DD329 pKa = 3.3 YY330 pKa = 11.61 WKK332 pKa = 11.1 VPNSWIIDD340 pKa = 3.63 AVNLSIEE347 pKa = 4.4 EE348 pKa = 4.06 SFAWIVTSPLLDD360 pKa = 4.7 AGWTYY365 pKa = 10.84 CGKK368 pKa = 10.04 IDD370 pKa = 4.48 GDD372 pKa = 3.52 EE373 pKa = 4.11 TRR375 pKa = 11.84 FGKK378 pKa = 8.99 SVRR381 pKa = 11.84 RR382 pKa = 11.84 KK383 pKa = 9.58 SVSTTSSPYY392 pKa = 11.28 YY393 pKa = 9.79 MDD395 pKa = 4.75 TNDD398 pKa = 3.29 STADD402 pKa = 4.54 FIPEE406 pKa = 4.32 TSPSLKK412 pKa = 10.61 NN413 pKa = 3.33
Molecular weight: 45.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.605
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.935
Patrickios 0.807
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A143XTZ2|A0A143XTZ2_9BACT Phosphoethanolamine transferase EptB OS=Alistipes sp. CHKCI003 OX=1780376 GN=eptB PE=4 SV=1
MM1 pKa = 7.84 PNGKK5 pKa = 8.55 KK6 pKa = 9.72 HH7 pKa = 6.1 KK8 pKa = 7.52 RR9 pKa = 11.84 HH10 pKa = 5.93 KK11 pKa = 9.95 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.95
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.676
Grimsley 12.676
Solomon 13.115
Lehninger 13.027
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.398
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2504
0
2504
798870
29
2392
319.0
35.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.81 ± 0.071
1.41 ± 0.021
5.645 ± 0.04
6.641 ± 0.049
4.209 ± 0.032
7.607 ± 0.048
1.79 ± 0.021
5.601 ± 0.042
4.53 ± 0.047
8.952 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.476 ± 0.024
3.485 ± 0.036
4.418 ± 0.033
2.824 ± 0.031
7.569 ± 0.079
5.723 ± 0.039
5.577 ± 0.045
6.865 ± 0.044
1.106 ± 0.017
3.76 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here