Alistipes sp. CHKCI003

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; unclassified Alistipes

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2504 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A143XFJ7|A0A143XFJ7_9BACT Outer membrane lipoprotein Blc OS=Alistipes sp. CHKCI003 OX=1780376 GN=blc PE=3 SV=1
MM1 pKa = 7.44SIKK4 pKa = 10.55HH5 pKa = 6.32LFYY8 pKa = 11.19ALAVSGMLSGAVSCADD24 pKa = 4.2DD25 pKa = 3.86EE26 pKa = 5.04PEE28 pKa = 4.25TEE30 pKa = 4.3VVPVDD35 pKa = 3.67FTLRR39 pKa = 11.84LNMPISLTDD48 pKa = 3.7PKK50 pKa = 10.13LTSVDD55 pKa = 3.73CAFTRR60 pKa = 11.84VEE62 pKa = 4.04TGDD65 pKa = 3.35EE66 pKa = 3.95VRR68 pKa = 11.84ITEE71 pKa = 4.11FTEE74 pKa = 4.54AEE76 pKa = 3.93TGAYY80 pKa = 8.86EE81 pKa = 4.34VVATLEE87 pKa = 4.0EE88 pKa = 4.29GTYY91 pKa = 10.53NIAVSGQIAYY101 pKa = 8.64TLDD104 pKa = 3.41GNTAEE109 pKa = 4.78RR110 pKa = 11.84DD111 pKa = 3.3IEE113 pKa = 4.01ASRR116 pKa = 11.84EE117 pKa = 4.09GVAVSAASDD126 pKa = 3.5VLTIEE131 pKa = 4.29TTVAAMKK138 pKa = 10.57SDD140 pKa = 3.48DD141 pKa = 3.92FVIEE145 pKa = 4.48EE146 pKa = 3.91IFYY149 pKa = 10.05TGTEE153 pKa = 4.28TPEE156 pKa = 3.66GMPYY160 pKa = 10.69YY161 pKa = 10.36GDD163 pKa = 3.14QYY165 pKa = 11.5MKK167 pKa = 9.5ITNNSDD173 pKa = 2.88RR174 pKa = 11.84TLYY177 pKa = 11.13ADD179 pKa = 3.48GLVIFKK185 pKa = 10.47SAFMTVEE192 pKa = 4.14KK193 pKa = 10.8YY194 pKa = 10.79DD195 pKa = 3.73YY196 pKa = 10.28TPDD199 pKa = 3.07IMSDD203 pKa = 3.73YY204 pKa = 10.72FSVDD208 pKa = 2.47SGLILPGSGTDD219 pKa = 3.54YY220 pKa = 10.77PVAPGASVVIADD232 pKa = 3.73TAIDD236 pKa = 3.5HH237 pKa = 6.64TEE239 pKa = 3.98FNSLSLDD246 pKa = 3.42LSGATFEE253 pKa = 4.74YY254 pKa = 10.76FNTLDD259 pKa = 5.37DD260 pKa = 4.6EE261 pKa = 6.0DD262 pKa = 4.46MDD264 pKa = 4.78NPDD267 pKa = 3.64VPNVTTLFADD277 pKa = 5.79DD278 pKa = 3.76FWTWHH283 pKa = 5.07NRR285 pKa = 11.84GFYY288 pKa = 9.51TYY290 pKa = 10.71AIGRR294 pKa = 11.84LGEE297 pKa = 4.24GVTAEE302 pKa = 4.57SFASDD307 pKa = 3.38YY308 pKa = 10.95AYY310 pKa = 9.23TVEE313 pKa = 4.21YY314 pKa = 10.75EE315 pKa = 4.39MVVPDD320 pKa = 4.02YY321 pKa = 10.53GTFPMSDD328 pKa = 4.6DD329 pKa = 3.3YY330 pKa = 11.61WKK332 pKa = 11.1VPNSWIIDD340 pKa = 3.63AVNLSIEE347 pKa = 4.4EE348 pKa = 4.06SFAWIVTSPLLDD360 pKa = 4.7AGWTYY365 pKa = 10.84CGKK368 pKa = 10.04IDD370 pKa = 4.48GDD372 pKa = 3.52EE373 pKa = 4.11TRR375 pKa = 11.84FGKK378 pKa = 8.99SVRR381 pKa = 11.84RR382 pKa = 11.84KK383 pKa = 9.58SVSTTSSPYY392 pKa = 11.28YY393 pKa = 9.79MDD395 pKa = 4.75TNDD398 pKa = 3.29STADD402 pKa = 4.54FIPEE406 pKa = 4.32TSPSLKK412 pKa = 10.61NN413 pKa = 3.33

Molecular weight:
45.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A143XTZ2|A0A143XTZ2_9BACT Phosphoethanolamine transferase EptB OS=Alistipes sp. CHKCI003 OX=1780376 GN=eptB PE=4 SV=1
MM1 pKa = 7.84PNGKK5 pKa = 8.55KK6 pKa = 9.72HH7 pKa = 6.1KK8 pKa = 7.52RR9 pKa = 11.84HH10 pKa = 5.93KK11 pKa = 9.95MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight:
3.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2504

0

2504

798870

29

2392

319.0

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 0.071

1.41 ± 0.021

5.645 ± 0.04

6.641 ± 0.049

4.209 ± 0.032

7.607 ± 0.048

1.79 ± 0.021

5.601 ± 0.042

4.53 ± 0.047

8.952 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.024

3.485 ± 0.036

4.418 ± 0.033

2.824 ± 0.031

7.569 ± 0.079

5.723 ± 0.039

5.577 ± 0.045

6.865 ± 0.044

1.106 ± 0.017

3.76 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski