Moraxella phage Mcat4
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PH31|A0A0R6PH31_9CAUD Uncharacterized protein OS=Moraxella phage Mcat4 OX=1647550 PE=4 SV=1
MM1 pKa = 7.84 KK2 pKa = 10.49 YY3 pKa = 10.16 MNDD6 pKa = 3.37 ACDD9 pKa = 3.32 TAEE12 pKa = 4.82 LNNPADD18 pKa = 3.92 GQGFVYY24 pKa = 9.74 PVIDD28 pKa = 4.16 GEE30 pKa = 4.55 CKK32 pKa = 9.85 IMRR35 pKa = 11.84 YY36 pKa = 10.31 DD37 pKa = 3.6 DD38 pKa = 4.58 FIKK41 pKa = 10.34 EE42 pKa = 4.06 FCFLPKK48 pKa = 10.3 DD49 pKa = 3.21 IRR51 pKa = 11.84 LNDD54 pKa = 3.47 EE55 pKa = 3.85
Molecular weight: 6.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.104
IPC2_protein 4.164
IPC_protein 4.062
Toseland 3.846
ProMoST 4.151
Dawson 4.05
Bjellqvist 4.291
Wikipedia 3.999
Rodwell 3.884
Grimsley 3.77
Solomon 4.037
Lehninger 3.986
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.91
EMBOSS 4.012
Sillero 4.177
Patrickios 3.121
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.113
Protein with the highest isoelectric point:
>tr|A0A0R6PCH1|A0A0R6PCH1_9CAUD Uncharacterized protein OS=Moraxella phage Mcat4 OX=1647550 PE=4 SV=1
MM1 pKa = 7.88 AFRR4 pKa = 11.84 IYY6 pKa = 10.57 KK7 pKa = 10.11 GDD9 pKa = 3.12 NHH11 pKa = 6.55 TPNSVFFIAHH21 pKa = 5.67 TPYY24 pKa = 10.92 LYY26 pKa = 10.97 GRR28 pKa = 11.84 GLWEE32 pKa = 3.78 CRR34 pKa = 11.84 KK35 pKa = 7.91 TRR37 pKa = 11.84 RR38 pKa = 11.84 FPLL41 pKa = 3.85
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.809
IPC_protein 10.482
Toseland 10.131
ProMoST 9.999
Dawson 10.409
Bjellqvist 10.175
Wikipedia 10.643
Rodwell 10.57
Grimsley 10.511
Solomon 10.467
Lehninger 10.438
Nozaki 10.175
DTASelect 10.16
Thurlkill 10.233
EMBOSS 10.555
Sillero 10.321
Patrickios 10.35
IPC_peptide 10.467
IPC2_peptide 9.297
IPC2.peptide.svr19 8.346
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
12784
39
1626
224.3
25.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.409 ± 0.534
0.993 ± 0.189
6.195 ± 0.252
5.202 ± 0.252
3.645 ± 0.211
6.641 ± 0.334
2.394 ± 0.274
7.079 ± 0.267
6.844 ± 0.354
8.495 ± 0.245
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.362 ± 0.228
5.436 ± 0.368
3.426 ± 0.322
4.678 ± 0.194
4.396 ± 0.237
6.735 ± 0.589
6.68 ± 0.422
5.757 ± 0.307
1.361 ± 0.104
3.27 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here