Podoviridae sp. ctfa10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4GUB7|A0A2Z4GUB7_9CAUD Upper collar protein OS=Podoviridae sp. ctfa10 OX=2675447 PE=4 SV=1
MM1 pKa = 7.67GMYY4 pKa = 10.0QYY6 pKa = 11.48DD7 pKa = 3.65STIFDD12 pKa = 5.08LFTLPDD18 pKa = 3.33EE19 pKa = 4.91MKK21 pKa = 10.66DD22 pKa = 3.32KK23 pKa = 11.03RR24 pKa = 11.84QDD26 pKa = 3.41IINSICLEE34 pKa = 4.15TQEE37 pKa = 6.01LEE39 pKa = 4.09VLYY42 pKa = 9.99PSTLSMQMAIGNWSNIMQSVWKK64 pKa = 10.4KK65 pKa = 9.81MYY67 pKa = 8.35NTTTLSYY74 pKa = 11.24NPIEE78 pKa = 5.05NYY80 pKa = 10.59DD81 pKa = 3.68KK82 pKa = 11.08TDD84 pKa = 3.28EE85 pKa = 4.3TTEE88 pKa = 4.02NEE90 pKa = 4.48TVSEE94 pKa = 4.28NITRR98 pKa = 11.84NDD100 pKa = 3.47TLKK103 pKa = 9.66NTTDD107 pKa = 3.07NTGNSEE113 pKa = 4.57IINQNVAYY121 pKa = 10.41NDD123 pKa = 3.44TSFTNNEE130 pKa = 3.68KK131 pKa = 10.17TINNVDD137 pKa = 3.23NNMVEE142 pKa = 4.2TGTNKK147 pKa = 10.13YY148 pKa = 9.04DD149 pKa = 3.64TDD151 pKa = 4.02RR152 pKa = 11.84SLTNGKK158 pKa = 8.21TITNRR163 pKa = 11.84SHH165 pKa = 6.73GNIGVTTSQQMIEE178 pKa = 4.07QEE180 pKa = 4.12RR181 pKa = 11.84QISEE185 pKa = 4.01FNIIKK190 pKa = 10.49YY191 pKa = 10.21IVDD194 pKa = 3.41DD195 pKa = 4.93FKK197 pKa = 11.61QKK199 pKa = 10.53FCLLIYY205 pKa = 10.65

Molecular weight: 23.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4GUK4|A0A2Z4GUK4_9CAUD Cpl-7 lysozyme domain-containing protein OS=Podoviridae sp. ctfa10 OX=2675447 PE=4 SV=1
MM1 pKa = 7.9PNGKK5 pKa = 9.39QGGKK9 pKa = 9.65RR10 pKa = 11.84EE11 pKa = 4.29FIKK14 pKa = 10.7LLRR17 pKa = 11.84DD18 pKa = 3.37RR19 pKa = 11.84GDD21 pKa = 3.48YY22 pKa = 10.53TPFAIQEE29 pKa = 3.96TWEE32 pKa = 3.95EE33 pKa = 4.0KK34 pKa = 9.57EE35 pKa = 3.78ARR37 pKa = 11.84KK38 pKa = 9.88EE39 pKa = 3.7YY40 pKa = 10.74SRR42 pKa = 11.84LRR44 pKa = 11.84NIVVKK49 pKa = 9.91RR50 pKa = 11.84LKK52 pKa = 10.31RR53 pKa = 11.84IEE55 pKa = 4.28EE56 pKa = 4.13VEE58 pKa = 3.89PDD60 pKa = 3.37AKK62 pKa = 10.44ILEE65 pKa = 4.31RR66 pKa = 11.84WKK68 pKa = 10.45PEE70 pKa = 3.73YY71 pKa = 10.05FKK73 pKa = 11.06KK74 pKa = 10.92LKK76 pKa = 10.09DD77 pKa = 3.23IKK79 pKa = 10.68NIRR82 pKa = 11.84EE83 pKa = 4.18LSHH86 pKa = 6.16MLSDD90 pKa = 3.67VAYY93 pKa = 10.26LVNAKK98 pKa = 8.15TASLTGRR105 pKa = 11.84HH106 pKa = 6.0EE107 pKa = 3.9IAIEE111 pKa = 4.26EE112 pKa = 4.11IEE114 pKa = 4.24KK115 pKa = 10.79LNKK118 pKa = 10.04FGIDD122 pKa = 2.98IKK124 pKa = 11.01NEE126 pKa = 3.91KK127 pKa = 8.6EE128 pKa = 3.87LKK130 pKa = 10.19EE131 pKa = 4.4FGNFMDD137 pKa = 5.64IARR140 pKa = 11.84DD141 pKa = 3.46WLKK144 pKa = 11.29DD145 pKa = 3.77RR146 pKa = 11.84IYY148 pKa = 11.04DD149 pKa = 3.91SEE151 pKa = 4.47RR152 pKa = 11.84LSTMYY157 pKa = 10.87AEE159 pKa = 4.24YY160 pKa = 10.92NEE162 pKa = 4.11RR163 pKa = 11.84FNNIEE168 pKa = 4.41LLRR171 pKa = 11.84AYY173 pKa = 10.06NKK175 pKa = 8.75WVKK178 pKa = 9.56NRR180 pKa = 11.84KK181 pKa = 7.73KK182 pKa = 10.31KK183 pKa = 10.87GYY185 pKa = 7.67ITHH188 pKa = 6.44KK189 pKa = 10.18RR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 3.62

Molecular weight: 23.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

4166

82

700

297.6

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.393 ± 0.388

1.8 ± 0.226

5.905 ± 0.351

6.937 ± 0.639

4.537 ± 0.335

5.977 ± 0.391

1.128 ± 0.221

7.177 ± 0.331

7.729 ± 0.66

7.201 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.048 ± 0.377

7.609 ± 0.647

2.928 ± 0.409

3.169 ± 0.6

3.865 ± 0.459

6.505 ± 0.31

6.649 ± 0.7

5.641 ± 0.315

1.608 ± 0.384

6.193 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski