Oceanobacillus bengalensis
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3792 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A494YR42|A0A494YR42_9BACI Uncharacterized protein OS=Oceanobacillus bengalensis OX=1435466 GN=D8M05_19845 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.32 KK3 pKa = 10.06 LLAFISILFVCFLAACGGNNDD24 pKa = 4.02 AEE26 pKa = 4.41 NGEE29 pKa = 4.2 EE30 pKa = 3.9 ASTIKK35 pKa = 10.69 VGASSVPHH43 pKa = 7.4 AEE45 pKa = 3.98 ILEE48 pKa = 4.26 EE49 pKa = 4.18 AVPLLEE55 pKa = 5.68 DD56 pKa = 3.35 EE57 pKa = 5.68 GITLEE62 pKa = 3.94 IEE64 pKa = 4.2 PYY66 pKa = 10.24 EE67 pKa = 5.23 DD68 pKa = 3.47 YY69 pKa = 11.39 VLPNDD74 pKa = 4.67 DD75 pKa = 4.86 LASGDD80 pKa = 3.52 LDD82 pKa = 4.64 ANFFQHH88 pKa = 6.8 VPFLNQTIADD98 pKa = 3.64 TGYY101 pKa = 8.93 EE102 pKa = 3.87 IEE104 pKa = 4.92 SIGEE108 pKa = 3.63 IHH110 pKa = 6.82 IEE112 pKa = 3.82 PMGVYY117 pKa = 9.31 SQNIKK122 pKa = 10.46 SVEE125 pKa = 4.12 EE126 pKa = 3.79 IAEE129 pKa = 4.15 GTEE132 pKa = 4.34 VILSNSIAEE141 pKa = 4.25 HH142 pKa = 5.78 GRR144 pKa = 11.84 ILSLFEE150 pKa = 3.91 QQGLLTLDD158 pKa = 3.52 EE159 pKa = 4.56 SVEE162 pKa = 4.07 KK163 pKa = 10.76 SAATIDD169 pKa = 5.15 DD170 pKa = 3.35 IVEE173 pKa = 4.07 NPKK176 pKa = 10.62 NLTFSHH182 pKa = 7.16 DD183 pKa = 3.7 FAPEE187 pKa = 3.91 FLPEE191 pKa = 4.7 LYY193 pKa = 10.31 HH194 pKa = 8.31 SEE196 pKa = 4.06 EE197 pKa = 4.02 EE198 pKa = 4.05 ALVVINTNYY207 pKa = 10.86 AIGAEE212 pKa = 4.22 LNPLEE217 pKa = 4.25 DD218 pKa = 4.82 AIFIEE223 pKa = 4.78 EE224 pKa = 4.48 EE225 pKa = 3.48 EE226 pKa = 4.24 SEE228 pKa = 4.94 YY229 pKa = 11.32 YY230 pKa = 10.58 NVIAVQSEE238 pKa = 4.28 DD239 pKa = 3.57 VNNEE243 pKa = 3.81 ALHH246 pKa = 6.26 KK247 pKa = 10.17 LVEE250 pKa = 4.19 VLISEE255 pKa = 5.41 DD256 pKa = 3.08 IQNFILEE263 pKa = 4.39 NYY265 pKa = 8.3 EE266 pKa = 3.84 GAVIPVGGNSS276 pKa = 3.05
Molecular weight: 30.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.63
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.592
Rodwell 3.63
Grimsley 3.541
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 3.961
Thurlkill 3.643
EMBOSS 3.617
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.745
IPC2_peptide 3.897
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A494YZ20|A0A494YZ20_9BACI OsmC family peroxiredoxin OS=Oceanobacillus bengalensis OX=1435466 GN=D8M05_10545 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.12 KK14 pKa = 8.46 VHH16 pKa = 5.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MSTKK25 pKa = 10.06 NGRR28 pKa = 11.84 KK29 pKa = 8.75 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.2 KK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.66 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.228
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.749
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.486
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3792
0
3792
1104151
14
1770
291.2
32.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.662 ± 0.04
0.634 ± 0.012
5.316 ± 0.029
7.81 ± 0.051
4.534 ± 0.035
6.72 ± 0.033
2.032 ± 0.022
8.49 ± 0.044
6.823 ± 0.04
9.539 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.798 ± 0.019
4.86 ± 0.033
3.414 ± 0.024
3.492 ± 0.024
3.866 ± 0.03
5.953 ± 0.03
5.516 ± 0.027
6.964 ± 0.03
0.978 ± 0.014
3.598 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here