Heliophilum fasciatum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae; Heliophilum

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2R7R9|A0A4R2R7R9_9FIRM Type III restriction/modification enzyme methylation subunit OS=Heliophilum fasciatum OX=35700 GN=EDD73_1572 PE=4 SV=1
MM1 pKa = 7.75LDD3 pKa = 3.77DD4 pKa = 4.96RR5 pKa = 11.84DD6 pKa = 3.73YY7 pKa = 11.69SADD10 pKa = 3.55GFHH13 pKa = 6.83ATDD16 pKa = 5.19ADD18 pKa = 3.85FTDD21 pKa = 3.91NYY23 pKa = 10.38IDD25 pKa = 5.32AEE27 pKa = 4.09PSYY30 pKa = 10.75QDD32 pKa = 2.87EE33 pKa = 4.52GFYY36 pKa = 11.11DD37 pKa = 5.2GIPRR41 pKa = 11.84II42 pKa = 4.07

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2RY77|A0A4R2RY77_9FIRM RND family efflux transporter MFP subunit OS=Heliophilum fasciatum OX=35700 GN=EDD73_11226 PE=3 SV=1
MM1 pKa = 7.89PYY3 pKa = 9.71IIHH6 pKa = 6.58RR7 pKa = 11.84NRR9 pKa = 11.84RR10 pKa = 11.84IFFEE14 pKa = 4.07EE15 pKa = 4.32RR16 pKa = 11.84GDD18 pKa = 3.9GAPLLLIHH26 pKa = 6.52GVAVSHH32 pKa = 6.65KK33 pKa = 8.4MWAPQLAVLARR44 pKa = 11.84SYY46 pKa = 11.1RR47 pKa = 11.84VIAPDD52 pKa = 3.3LRR54 pKa = 11.84GAGKK58 pKa = 10.47SSGLSWQSRR67 pKa = 11.84VADD70 pKa = 4.35LVADD74 pKa = 5.31LIALLDD80 pKa = 3.75RR81 pKa = 11.84LAIEE85 pKa = 4.13QVAVCGLSFGGVVAQEE101 pKa = 3.74LVLRR105 pKa = 11.84YY106 pKa = 8.96PEE108 pKa = 4.22RR109 pKa = 11.84CAMLVLVDD117 pKa = 4.07TFSEE121 pKa = 4.14LRR123 pKa = 11.84PGISQEE129 pKa = 3.93GALWLATWLGVPLFLMPKK147 pKa = 8.07RR148 pKa = 11.84WMLARR153 pKa = 11.84MKK155 pKa = 10.12EE156 pKa = 4.56SYY158 pKa = 10.65GKK160 pKa = 9.48WPVACRR166 pKa = 11.84CLTAEE171 pKa = 3.93IPKK174 pKa = 9.69IRR176 pKa = 11.84GMEE179 pKa = 4.05AVKK182 pKa = 9.67MRR184 pKa = 11.84WMMRR188 pKa = 11.84GLRR191 pKa = 11.84LTPRR195 pKa = 11.84LAAIHH200 pKa = 6.28CPCLGIVGGNTALGIALMRR219 pKa = 11.84RR220 pKa = 11.84VIEE223 pKa = 4.92AIPGAKK229 pKa = 9.69LRR231 pKa = 11.84VVPHH235 pKa = 6.68AFDD238 pKa = 4.03PTSLCQPAIFNRR250 pKa = 11.84LVDD253 pKa = 3.9EE254 pKa = 6.02FLAPWQEE261 pKa = 3.36RR262 pKa = 11.84WAARR266 pKa = 11.84SSEE269 pKa = 3.91RR270 pKa = 11.84FLRR273 pKa = 11.84KK274 pKa = 9.28ISARR278 pKa = 11.84ARR280 pKa = 11.84NRR282 pKa = 11.84QSICRR287 pKa = 11.84SRR289 pKa = 11.84WEE291 pKa = 3.96PSFITLGVGQRR302 pKa = 11.84NAKK305 pKa = 10.36DD306 pKa = 3.23GAEE309 pKa = 3.94RR310 pKa = 3.8

Molecular weight: 34.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2911

0

2911

922013

26

2401

316.7

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.532 ± 0.065

1.061 ± 0.019

5.13 ± 0.033

6.53 ± 0.047

3.575 ± 0.03

7.53 ± 0.051

2.092 ± 0.025

6.267 ± 0.04

4.759 ± 0.042

10.013 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.734 ± 0.021

3.348 ± 0.033

4.609 ± 0.038

4.56 ± 0.04

5.909 ± 0.037

5.195 ± 0.034

5.606 ± 0.034

7.392 ± 0.041

1.235 ± 0.018

2.922 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski