Streptococcus satellite phage Javan428

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMV1|A0A4D5ZMV1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan428 OX=2558696 GN=JavanS428_0012 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.34YY3 pKa = 8.99TYY5 pKa = 10.63LALFEE10 pKa = 4.37VDD12 pKa = 4.36EE13 pKa = 4.63EE14 pKa = 4.51NGGYY18 pKa = 9.13TITFPDD24 pKa = 3.82FQGAISEE31 pKa = 4.32ADD33 pKa = 3.59TLNEE37 pKa = 4.16AIYY40 pKa = 10.24NARR43 pKa = 11.84EE44 pKa = 3.69VLEE47 pKa = 4.72IYY49 pKa = 10.37TIMFEE54 pKa = 4.86DD55 pKa = 3.72EE56 pKa = 4.19GKK58 pKa = 10.45EE59 pKa = 3.99FPEE62 pKa = 4.4PSSFKK67 pKa = 10.97ALASEE72 pKa = 4.7LGSDD76 pKa = 4.01EE77 pKa = 6.27DD78 pKa = 3.51ILQAISVDD86 pKa = 3.58TEE88 pKa = 3.93LVRR91 pKa = 11.84EE92 pKa = 4.2RR93 pKa = 11.84EE94 pKa = 3.81RR95 pKa = 11.84SRR97 pKa = 11.84VVNKK101 pKa = 9.36TVTLPSWLVEE111 pKa = 3.9VGKK114 pKa = 10.22EE115 pKa = 3.77NKK117 pKa = 9.75INFSQLLQKK126 pKa = 10.63AIRR129 pKa = 11.84EE130 pKa = 4.1EE131 pKa = 4.21LQVV134 pKa = 3.26

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMJ4|A0A4D5ZMJ4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan428 OX=2558696 GN=JavanS428_0011 PE=4 SV=1
MM1 pKa = 7.33NSEE4 pKa = 4.83ASQQLSDD11 pKa = 3.93SRR13 pKa = 11.84FKK15 pKa = 11.32SLVGVQRR22 pKa = 11.84TTFEE26 pKa = 3.69EE27 pKa = 4.04MLAVLKK33 pKa = 9.28TAYY36 pKa = 9.06QRR38 pKa = 11.84KK39 pKa = 7.87RR40 pKa = 11.84AKK42 pKa = 10.3GGRR45 pKa = 11.84KK46 pKa = 8.91PKK48 pKa = 10.66LSLDD52 pKa = 3.85DD53 pKa = 4.56LLMVTIQYY61 pKa = 8.63MRR63 pKa = 11.84EE64 pKa = 3.83

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3169

41

507

144.0

16.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.333 ± 0.334

0.41 ± 0.125

5.869 ± 0.33

9.12 ± 0.773

3.85 ± 0.464

5.112 ± 0.385

1.325 ± 0.28

7.132 ± 0.466

9.34 ± 0.738

10.192 ± 0.604

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.248

5.08 ± 0.295

2.935 ± 0.465

4.323 ± 0.367

5.238 ± 0.308

5.775 ± 0.279

6.059 ± 0.448

5.396 ± 0.409

0.82 ± 0.174

4.512 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski