Bordetella phage vB_BbrP_BB8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; unclassified Autographiviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TPV3|A0A4Y5TPV3_9CAUD Uncharacterized protein OS=Bordetella phage vB_BbrP_BB8 OX=2587820 GN=bb8_p18 PE=4 SV=1
MM1 pKa = 7.89SEE3 pKa = 3.96GSLEE7 pKa = 3.75NSYY10 pKa = 11.33YY11 pKa = 10.96VVLADD16 pKa = 5.58EE17 pKa = 5.42NIQLANSEE25 pKa = 4.19FSVRR29 pKa = 3.79

Molecular weight: 3.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TQY8|A0A4Y5TQY8_9CAUD Uncharacterized protein OS=Bordetella phage vB_BbrP_BB8 OX=2587820 GN=bb8_p25 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84SLLRR6 pKa = 11.84RR7 pKa = 11.84VAPEE11 pKa = 3.7SLPTRR16 pKa = 11.84EE17 pKa = 4.64APGATPLNGEE27 pKa = 4.33CWVVTGRR34 pKa = 11.84TKK36 pKa = 11.05

Molecular weight: 3.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12812

29

1551

224.8

24.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.1 ± 0.371

0.952 ± 0.154

5.948 ± 0.273

6.72 ± 0.383

3.676 ± 0.192

8.125 ± 0.344

1.748 ± 0.2

4.644 ± 0.18

5.659 ± 0.348

8.5 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.143

3.848 ± 0.285

4.558 ± 0.246

4.098 ± 0.247

5.893 ± 0.261

5.542 ± 0.37

5.924 ± 0.314

7.134 ± 0.244

1.342 ± 0.101

3.27 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski