Staphylococcus phage f2b1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; unclassified Bastillevirinae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0RRN9|A0A6C0RRN9_9CAUD Uncharacterized protein OS=Staphylococcus phage f2b1 OX=2708592 PE=4 SV=1
MM1 pKa = 7.45FGLTEE6 pKa = 4.33YY7 pKa = 10.64EE8 pKa = 4.02LEE10 pKa = 4.45TYY12 pKa = 10.62KK13 pKa = 11.15SLTQDD18 pKa = 3.62FAITEE23 pKa = 4.17ATPTLAAKK31 pKa = 9.95AQFMVPAEE39 pKa = 3.87IRR41 pKa = 11.84GEE43 pKa = 4.05KK44 pKa = 9.97VIPDD48 pKa = 3.87MPYY51 pKa = 10.2LNQMKK56 pKa = 9.72TDD58 pKa = 4.3FEE60 pKa = 5.17TIVTLTATSMAYY72 pKa = 10.09DD73 pKa = 3.93YY74 pKa = 11.83AHH76 pKa = 7.31LLAEE80 pKa = 4.55SSLKK84 pKa = 10.71DD85 pKa = 3.33SEE87 pKa = 4.98IIDD90 pKa = 4.1KK91 pKa = 10.35IYY93 pKa = 10.81SKK95 pKa = 10.9HH96 pKa = 5.24EE97 pKa = 4.5SYY99 pKa = 11.97VLMQFIKK106 pKa = 10.64YY107 pKa = 10.04GIVFTDD113 pKa = 3.26EE114 pKa = 4.42SIRR117 pKa = 11.84EE118 pKa = 3.86IVGIIILEE126 pKa = 4.05LPYY129 pKa = 10.8LYY131 pKa = 9.94MAVIEE136 pKa = 4.31EE137 pKa = 4.9GEE139 pKa = 4.4LEE141 pKa = 4.92DD142 pKa = 5.41DD143 pKa = 4.7DD144 pKa = 4.91EE145 pKa = 5.78VEE147 pKa = 4.65ARR149 pKa = 11.84LEE151 pKa = 3.94AYY153 pKa = 10.18YY154 pKa = 10.67DD155 pKa = 3.99YY156 pKa = 11.42VQEE159 pKa = 4.58NYY161 pKa = 9.34PQEE164 pKa = 4.77GEE166 pKa = 3.96

Molecular weight: 19.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0RU29|A0A6C0RU29_9CAUD Uncharacterized protein OS=Staphylococcus phage f2b1 OX=2708592 PE=4 SV=1
MM1 pKa = 7.42GNSNSFNRR9 pKa = 11.84SKK11 pKa = 11.16SRR13 pKa = 11.84GTEE16 pKa = 3.69ARR18 pKa = 11.84GMTGLNGNGKK28 pKa = 9.23DD29 pKa = 3.52AYY31 pKa = 9.65KK32 pKa = 10.7KK33 pKa = 10.49SLQSTKK39 pKa = 9.12GHH41 pKa = 5.48YY42 pKa = 9.35RR43 pKa = 11.84VAFTRR48 pKa = 11.84SFSQVTEE55 pKa = 3.7KK56 pKa = 10.88DD57 pKa = 2.7IEE59 pKa = 4.16LRR61 pKa = 11.84KK62 pKa = 10.08KK63 pKa = 10.47YY64 pKa = 10.56AIDD67 pKa = 4.99LISEE71 pKa = 4.76EE72 pKa = 4.27IAVPTDD78 pKa = 3.75IITLRR83 pKa = 11.84AKK85 pKa = 10.22RR86 pKa = 11.84DD87 pKa = 3.52SGEE90 pKa = 3.95QTLTARR96 pKa = 11.84DD97 pKa = 3.21EE98 pKa = 4.21VFYY101 pKa = 11.38VKK103 pKa = 10.47VGATNCGKK111 pKa = 10.31ISIRR115 pKa = 11.84TQRR118 pKa = 11.84HH119 pKa = 3.49WKK121 pKa = 8.39GTILMFIFQTKK132 pKa = 9.38GAPLRR137 pKa = 11.84EE138 pKa = 4.13KK139 pKa = 10.87KK140 pKa = 10.41KK141 pKa = 10.56GFTRR145 pKa = 11.84KK146 pKa = 9.37VGYY149 pKa = 9.77KK150 pKa = 9.69KK151 pKa = 10.2RR152 pKa = 11.84SKK154 pKa = 10.76SQVQASKK161 pKa = 10.83RR162 pKa = 11.84KK163 pKa = 9.7AYY165 pKa = 10.57NNRR168 pKa = 11.84KK169 pKa = 10.05GGTNN173 pKa = 3.14

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

224

0

224

48878

36

1260

218.2

24.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.784 ± 0.151

0.737 ± 0.062

6.128 ± 0.099

7.398 ± 0.3

3.983 ± 0.102

6.856 ± 0.283

1.778 ± 0.088

6.396 ± 0.132

7.38 ± 0.234

7.871 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.088

5.475 ± 0.174

3.263 ± 0.127

3.728 ± 0.134

4.405 ± 0.108

5.972 ± 0.192

6.733 ± 0.271

7.054 ± 0.161

1.0 ± 0.046

4.395 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski