Amino acid dipepetide frequency for Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.434AlaAla: 4.434 ± 0.082	0.705AlaCys: 0.705 ± 0.028	2.669AlaAsp: 2.669 ± 0.051	3.385AlaGlu: 3.385 ± 0.062	2.635AlaPhe: 2.635 ± 0.055	3.736AlaGly: 3.736 ± 0.077	0.835AlaHis: 0.835 ± 0.03	5.444AlaIle: 5.444 ± 0.078	4.627AlaLys: 4.627 ± 0.066	5.629AlaLeu: 5.629 ± 0.08	1.539AlaMet: 1.539 ± 0.039	2.73AlaAsn: 2.73 ± 0.052	1.44AlaPro: 1.44 ± 0.039	1.391AlaGln: 1.391 ± 0.04	1.817AlaArg: 1.817 ± 0.04	3.984AlaSer: 3.984 ± 0.063	2.547AlaThr: 2.547 ± 0.059	4.432AlaVal: 4.432 ± 0.068	0.399AlaTrp: 0.399 ± 0.021	2.282AlaTyr: 2.282 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.615CysAla: 0.615 ± 0.026	0.182CysCys: 0.182 ± 0.013	0.705CysAsp: 0.705 ± 0.024	0.739CysGlu: 0.739 ± 0.024	0.592CysPhe: 0.592 ± 0.025	1.019CysGly: 1.019 ± 0.033	0.218CysHis: 0.218 ± 0.015	1.199CysIle: 1.199 ± 0.034	0.936CysLys: 0.936 ± 0.031	0.901CysLeu: 0.901 ± 0.031	0.289CysMet: 0.289 ± 0.014	0.661CysAsn: 0.661 ± 0.025	0.443CysPro: 0.443 ± 0.023	0.222CysGln: 0.222 ± 0.016	0.405CysArg: 0.405 ± 0.021	0.964CysSer: 0.964 ± 0.032	0.619CysThr: 0.619 ± 0.026	0.726CysVal: 0.726 ± 0.023	0.086CysTrp: 0.086 ± 0.008	0.481CysTyr: 0.481 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.878AspAla: 2.878 ± 0.05	0.592AspCys: 0.592 ± 0.024	2.916AspAsp: 2.916 ± 0.059	4.318AspGlu: 4.318 ± 0.072	2.827AspPhe: 2.827 ± 0.051	3.645AspGly: 3.645 ± 0.085	0.528AspHis: 0.528 ± 0.024	5.947AspIle: 5.947 ± 0.082	5.59AspLys: 5.59 ± 0.076	4.418AspLeu: 4.418 ± 0.061	1.55AspMet: 1.55 ± 0.034	3.533AspAsn: 3.533 ± 0.06	1.291AspPro: 1.291 ± 0.038	0.805AspGln: 0.805 ± 0.031	1.769AspArg: 1.769 ± 0.04	3.385AspSer: 3.385 ± 0.068	2.609AspThr: 2.609 ± 0.054	3.643AspVal: 3.643 ± 0.055	0.411AspTrp: 0.411 ± 0.019	2.772AspTyr: 2.772 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.204GluAla: 4.204 ± 0.08	0.714GluCys: 0.714 ± 0.023	3.887GluAsp: 3.887 ± 0.065	5.805GluGlu: 5.805 ± 0.108	2.92GluPhe: 2.92 ± 0.051	3.62GluGly: 3.62 ± 0.054	0.843GluHis: 0.843 ± 0.03	6.465GluIle: 6.465 ± 0.1	7.366GluLys: 7.366 ± 0.112	5.988GluLeu: 5.988 ± 0.092	1.695GluMet: 1.695 ± 0.043	5.191GluAsn: 5.191 ± 0.076	1.316GluPro: 1.316 ± 0.035	1.543GluGln: 1.543 ± 0.044	2.436GluArg: 2.436 ± 0.049	3.305GluSer: 3.305 ± 0.059	2.771GluThr: 2.771 ± 0.052	4.452GluVal: 4.452 ± 0.08	0.431GluTrp: 0.431 ± 0.019	2.811GluTyr: 2.811 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.21PheAla: 2.21 ± 0.048	0.58PheCys: 0.58 ± 0.025	2.547PheAsp: 2.547 ± 0.056	2.647PheGlu: 2.647 ± 0.046	2.098PhePhe: 2.098 ± 0.05	2.863PheGly: 2.863 ± 0.057	0.593PheHis: 0.593 ± 0.023	4.492PheIle: 4.492 ± 0.086	4.137PheLys: 4.137 ± 0.062	3.988PheLeu: 3.988 ± 0.076	1.155PheMet: 1.155 ± 0.032	3.103PheAsn: 3.103 ± 0.071	1.17PhePro: 1.17 ± 0.032	1.022PheGln: 1.022 ± 0.031	1.265PheArg: 1.265 ± 0.032	3.547PheSer: 3.547 ± 0.056	2.243PheThr: 2.243 ± 0.046	2.863PheVal: 2.863 ± 0.048	0.329PheTrp: 0.329 ± 0.02	1.996PheTyr: 1.996 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	3.892GlyAla: 3.892 ± 0.075	0.856GlyCys: 0.856 ± 0.03	3.239GlyAsp: 3.239 ± 0.063	4.045GlyGlu: 4.045 ± 0.07	3.105GlyPhe: 3.105 ± 0.062	4.057GlyGly: 4.057 ± 0.072	0.948GlyHis: 0.948 ± 0.032	6.61GlyIle: 6.61 ± 0.086	5.755GlyLys: 5.755 ± 0.08	4.881GlyLeu: 4.881 ± 0.081	1.707GlyMet: 1.707 ± 0.04	3.508GlyAsn: 3.508 ± 0.064	1.154GlyPro: 1.154 ± 0.036	1.573GlyGln: 1.573 ± 0.04	2.205GlyArg: 2.205 ± 0.046	3.949GlySer: 3.949 ± 0.07	3.683GlyThr: 3.683 ± 0.077	4.566GlyVal: 4.566 ± 0.082	0.659GlyTrp: 0.659 ± 0.047	2.919GlyTyr: 2.919 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	0.626HisAla: 0.626 ± 0.024	0.198HisCys: 0.198 ± 0.012	0.718HisAsp: 0.718 ± 0.027	0.841HisGlu: 0.841 ± 0.028	0.62HisPhe: 0.62 ± 0.026	0.955HisGly: 0.955 ± 0.033	0.319HisHis: 0.319 ± 0.018	1.288HisIle: 1.288 ± 0.031	1.075HisLys: 1.075 ± 0.033	1.084HisLeu: 1.084 ± 0.029	0.376HisMet: 0.376 ± 0.02	0.824HisAsn: 0.824 ± 0.028	0.587HisPro: 0.587 ± 0.021	0.287HisGln: 0.287 ± 0.015	0.495HisArg: 0.495 ± 0.019	0.875HisSer: 0.875 ± 0.027	0.693HisThr: 0.693 ± 0.021	0.867HisVal: 0.867 ± 0.036	0.113HisTrp: 0.113 ± 0.009	0.578HisTyr: 0.578 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.458IleAla: 5.458 ± 0.087	1.319IleCys: 1.319 ± 0.032	5.629IleAsp: 5.629 ± 0.077	6.434IleGlu: 6.434 ± 0.084	4.321IlePhe: 4.321 ± 0.074	5.85IleGly: 5.85 ± 0.083	1.181IleHis: 1.181 ± 0.035	8.886IleIle: 8.886 ± 0.125	9.052IleLys: 9.052 ± 0.097	8.614IleLeu: 8.614 ± 0.115	2.343IleMet: 2.343 ± 0.054	6.334IleAsn: 6.334 ± 0.083	3.04IlePro: 3.04 ± 0.047	2.083IleGln: 2.083 ± 0.044	3.1IleArg: 3.1 ± 0.064	7.111IleSer: 7.111 ± 0.093	4.93IleThr: 4.93 ± 0.074	6.323IleVal: 6.323 ± 0.087	0.547IleTrp: 0.547 ± 0.023	3.661IleTyr: 3.661 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	5.242LysAla: 5.242 ± 0.078	1.005LysCys: 1.005 ± 0.031	5.906LysAsp: 5.906 ± 0.068	7.778LysGlu: 7.778 ± 0.105	3.442LysPhe: 3.442 ± 0.059	5.184LysGly: 5.184 ± 0.058	1.179LysHis: 1.179 ± 0.038	8.58LysIle: 8.58 ± 0.107	8.938LysLys: 8.938 ± 0.098	8.053LysLeu: 8.053 ± 0.082	2.545LysMet: 2.545 ± 0.043	7.028LysAsn: 7.028 ± 0.09	2.152LysPro: 2.152 ± 0.045	2.154LysGln: 2.154 ± 0.051	3.319LysArg: 3.319 ± 0.054	5.769LysSer: 5.769 ± 0.086	4.31LysThr: 4.31 ± 0.064	6.289LysVal: 6.289 ± 0.075	0.689LysTrp: 0.689 ± 0.026	4.295LysTyr: 4.295 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	4.608LeuAla: 4.608 ± 0.063	1.108LeuCys: 1.108 ± 0.031	4.597LeuAsp: 4.597 ± 0.06	5.421LeuGlu: 5.421 ± 0.092	3.609LeuPhe: 3.609 ± 0.072	5.653LeuGly: 5.653 ± 0.079	1.062LeuHis: 1.062 ± 0.031	7.85LeuIle: 7.85 ± 0.095	9.215LeuLys: 9.215 ± 0.094	7.131LeuLeu: 7.131 ± 0.105	2.274LeuMet: 2.274 ± 0.044	6.21LeuAsn: 6.21 ± 0.088	2.587LeuPro: 2.587 ± 0.05	2.09LeuGln: 2.09 ± 0.045	3.18LeuArg: 3.18 ± 0.055	6.673LeuSer: 6.673 ± 0.09	4.37LeuThr: 4.37 ± 0.068	5.044LeuVal: 5.044 ± 0.07	0.581LeuTrp: 0.581 ± 0.026	3.3LeuTyr: 3.3 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.654MetAla: 1.654 ± 0.041	0.28MetCys: 0.28 ± 0.013	1.577MetAsp: 1.577 ± 0.041	1.728MetGlu: 1.728 ± 0.048	1.087MetPhe: 1.087 ± 0.036	1.718MetGly: 1.718 ± 0.042	0.364MetHis: 0.364 ± 0.017	2.08MetIle: 2.08 ± 0.046	2.64MetLys: 2.64 ± 0.045	2.347MetLeu: 2.347 ± 0.044	0.644MetMet: 0.644 ± 0.022	1.719MetAsn: 1.719 ± 0.032	0.871MetPro: 0.871 ± 0.029	0.686MetGln: 0.686 ± 0.035	0.863MetArg: 0.863 ± 0.031	1.704MetSer: 1.704 ± 0.039	1.169MetThr: 1.169 ± 0.028	1.592MetVal: 1.592 ± 0.038	0.174MetTrp: 0.174 ± 0.012	0.96MetTyr: 0.96 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.256AsnAla: 3.256 ± 0.055	0.761AsnCys: 0.761 ± 0.029	3.252AsnAsp: 3.252 ± 0.057	4.158AsnGlu: 4.158 ± 0.067	2.752AsnPhe: 2.752 ± 0.055	4.138AsnGly: 4.138 ± 0.097	0.809AsnHis: 0.809 ± 0.024	6.979AsnIle: 6.979 ± 0.079	6.459AsnLys: 6.459 ± 0.08	5.743AsnLeu: 5.743 ± 0.083	1.76AsnMet: 1.76 ± 0.039	4.515AsnAsn: 4.515 ± 0.093	2.144AsnPro: 2.144 ± 0.041	1.441AsnGln: 1.441 ± 0.039	1.944AsnArg: 1.944 ± 0.042	4.598AsnSer: 4.598 ± 0.074	3.269AsnThr: 3.269 ± 0.063	4.077AsnVal: 4.077 ± 0.065	0.529AsnTrp: 0.529 ± 0.023	2.994AsnTyr: 2.994 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	1.369ProAla: 1.369 ± 0.04	0.31ProCys: 0.31 ± 0.017	1.48ProAsp: 1.48 ± 0.035	2.012ProGlu: 2.012 ± 0.051	1.352ProPhe: 1.352 ± 0.036	1.58ProGly: 1.58 ± 0.042	0.486ProHis: 0.486 ± 0.022	2.595ProIle: 2.595 ± 0.041	2.27ProLys: 2.27 ± 0.044	2.377ProLeu: 2.377 ± 0.04	0.672ProMet: 0.672 ± 0.025	1.65ProAsn: 1.65 ± 0.034	0.627ProPro: 0.627 ± 0.022	0.778ProGln: 0.778 ± 0.024	0.773ProArg: 0.773 ± 0.028	1.845ProSer: 1.845 ± 0.043	1.49ProThr: 1.49 ± 0.036	2.035ProVal: 2.035 ± 0.054	0.253ProTrp: 0.253 ± 0.013	1.291ProTyr: 1.291 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.39GlnAla: 1.39 ± 0.037	0.241GlnCys: 0.241 ± 0.015	1.314GlnAsp: 1.314 ± 0.034	1.419GlnGlu: 1.419 ± 0.042	1.027GlnPhe: 1.027 ± 0.025	1.374GlnGly: 1.374 ± 0.038	0.301GlnHis: 0.301 ± 0.018	2.135GlnIle: 2.135 ± 0.045	2.139GlnLys: 2.139 ± 0.052	1.94GlnLeu: 1.94 ± 0.043	0.647GlnMet: 0.647 ± 0.022	1.775GlnAsn: 1.775 ± 0.046	0.594GlnPro: 0.594 ± 0.023	0.716GlnGln: 0.716 ± 0.036	0.889GlnArg: 0.889 ± 0.03	1.495GlnSer: 1.495 ± 0.037	1.185GlnThr: 1.185 ± 0.062	1.439GlnVal: 1.439 ± 0.036	0.217GlnTrp: 0.217 ± 0.016	1.138GlnTyr: 1.138 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	1.873ArgAla: 1.873 ± 0.039	0.399ArgCys: 0.399 ± 0.02	1.77ArgAsp: 1.77 ± 0.047	2.733ArgGlu: 2.733 ± 0.062	1.436ArgPhe: 1.436 ± 0.035	1.938ArgGly: 1.938 ± 0.046	0.494ArgHis: 0.494 ± 0.02	3.272ArgIle: 3.272 ± 0.063	3.129ArgLys: 3.129 ± 0.055	2.855ArgLeu: 2.855 ± 0.057	0.926ArgMet: 0.926 ± 0.03	2.131ArgAsn: 2.131 ± 0.048	0.835ArgPro: 0.835 ± 0.028	0.923ArgGln: 0.923 ± 0.031	1.506ArgArg: 1.506 ± 0.041	1.689ArgSer: 1.689 ± 0.041	1.548ArgThr: 1.548 ± 0.034	2.286ArgVal: 2.286 ± 0.05	0.253ArgTrp: 0.253 ± 0.015	1.486ArgTyr: 1.486 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.595SerAla: 3.595 ± 0.07	0.685SerCys: 0.685 ± 0.027	3.6SerAsp: 3.6 ± 0.068	4.168SerGlu: 4.168 ± 0.07	3.261SerPhe: 3.261 ± 0.054	4.694SerGly: 4.694 ± 0.069	0.866SerHis: 0.866 ± 0.025	6.87SerIle: 6.87 ± 0.098	6.387SerLys: 6.387 ± 0.087	5.849SerLeu: 5.849 ± 0.088	1.694SerMet: 1.694 ± 0.039	4.253SerAsn: 4.253 ± 0.064	1.696SerPro: 1.696 ± 0.043	1.703SerGln: 1.703 ± 0.042	2.197SerArg: 2.197 ± 0.05	5.282SerSer: 5.282 ± 0.101	3.507SerThr: 3.507 ± 0.054	4.308SerVal: 4.308 ± 0.06	0.496SerTrp: 0.496 ± 0.021	2.954SerTyr: 2.954 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.231ThrAla: 3.231 ± 0.066	0.511ThrCys: 0.511 ± 0.022	2.623ThrAsp: 2.623 ± 0.059	2.806ThrGlu: 2.806 ± 0.058	2.192ThrPhe: 2.192 ± 0.043	3.705ThrGly: 3.705 ± 0.098	0.752ThrHis: 0.752 ± 0.028	4.67ThrIle: 4.67 ± 0.071	3.755ThrLys: 3.755 ± 0.064	4.685ThrLeu: 4.685 ± 0.072	1.087ThrMet: 1.087 ± 0.027	2.926ThrAsn: 2.926 ± 0.055	1.822ThrPro: 1.822 ± 0.041	1.146ThrGln: 1.146 ± 0.035	1.492ThrArg: 1.492 ± 0.037	3.547ThrSer: 3.547 ± 0.06	2.709ThrThr: 2.709 ± 0.058	3.53ThrVal: 3.53 ± 0.078	0.407ThrTrp: 0.407 ± 0.021	2.103ThrTyr: 2.103 ± 0.065	0.0ThrXaa: 0.0 ± 0.0
Val	3.635ValAla: 3.635 ± 0.067	0.88ValCys: 0.88 ± 0.03	3.922ValAsp: 3.922 ± 0.06	4.192ValGlu: 4.192 ± 0.074	3.077ValPhe: 3.077 ± 0.058	4.286ValGly: 4.286 ± 0.08	0.895ValHis: 0.895 ± 0.026	6.163ValIle: 6.163 ± 0.085	5.779ValLys: 5.779 ± 0.077	5.906ValLeu: 5.906 ± 0.083	1.659ValMet: 1.659 ± 0.039	4.07ValAsn: 4.07 ± 0.07	2.094ValPro: 2.094 ± 0.043	1.645ValGln: 1.645 ± 0.047	2.058ValArg: 2.058 ± 0.044	4.712ValSer: 4.712 ± 0.062	3.537ValThr: 3.537 ± 0.061	4.669ValVal: 4.669 ± 0.079	0.406ValTrp: 0.406 ± 0.019	2.59ValTyr: 2.59 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.384TrpAla: 0.384 ± 0.019	0.116TrpCys: 0.116 ± 0.011	0.405TrpAsp: 0.405 ± 0.021	0.389TrpGlu: 0.389 ± 0.018	0.36TrpPhe: 0.36 ± 0.021	0.567TrpGly: 0.567 ± 0.023	0.153TrpHis: 0.153 ± 0.012	0.682TrpIle: 0.682 ± 0.031	0.569TrpLys: 0.569 ± 0.024	0.59TrpLeu: 0.59 ± 0.023	0.217TrpMet: 0.217 ± 0.014	0.487TrpAsn: 0.487 ± 0.022	0.151TrpPro: 0.151 ± 0.012	0.326TrpGln: 0.326 ± 0.03	0.266TrpArg: 0.266 ± 0.016	0.501TrpSer: 0.501 ± 0.021	0.36TrpThr: 0.36 ± 0.02	0.453TrpVal: 0.453 ± 0.025	0.105TrpTrp: 0.105 ± 0.011	0.326TrpTyr: 0.326 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.108TyrAla: 2.108 ± 0.04	0.557TyrCys: 0.557 ± 0.023	2.672TyrAsp: 2.672 ± 0.053	2.743TyrGlu: 2.743 ± 0.053	2.136TyrPhe: 2.136 ± 0.075	2.728TyrGly: 2.728 ± 0.052	0.591TyrHis: 0.591 ± 0.024	4.009TyrIle: 4.009 ± 0.063	4.039TyrLys: 4.039 ± 0.063	3.588TyrLeu: 3.588 ± 0.049	1.079TyrMet: 1.079 ± 0.033	2.93TyrAsn: 2.93 ± 0.054	1.23TyrPro: 1.23 ± 0.034	0.844TyrGln: 0.844 ± 0.031	1.505TyrArg: 1.505 ± 0.04	3.131TyrSer: 3.131 ± 0.055	2.147TyrThr: 2.147 ± 0.045	2.583TyrVal: 2.583 ± 0.05	0.318TyrTrp: 0.318 ± 0.018	2.115TyrTyr: 2.115 ± 0.101	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3847 proteins (1176635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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