Polygonum ringspot tospovirus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4GW61|A4GW61_9VIRU Nucleoprotein OS=Polygonum ringspot tospovirus OX=430606 GN=N PE=3 SV=1
MM1 pKa = 7.07 NRR3 pKa = 11.84 HH4 pKa = 5.7 YY5 pKa = 11.29 LLLYY9 pKa = 10.23 CLGVYY14 pKa = 10.67 LMFTITEE21 pKa = 4.18 VFLMNHH27 pKa = 6.42 AEE29 pKa = 3.99 DD30 pKa = 4.87 HH31 pKa = 6.88 EE32 pKa = 4.34 DD33 pKa = 3.28 TKK35 pKa = 11.51 VEE37 pKa = 4.14 LKK39 pKa = 10.45 RR40 pKa = 11.84 LKK42 pKa = 10.41 DD43 pKa = 3.28 RR44 pKa = 11.84 YY45 pKa = 10.62 KK46 pKa = 10.3 IDD48 pKa = 4.01 DD49 pKa = 4.57 PEE51 pKa = 6.15 DD52 pKa = 3.65 LLEE55 pKa = 5.83 DD56 pKa = 4.21 SIKK59 pKa = 10.16 DD60 pKa = 3.37 TGMVATSGAHH70 pKa = 6.58 PSLEE74 pKa = 4.32 SKK76 pKa = 9.82 TSGRR80 pKa = 11.84 SPRR83 pKa = 11.84 EE84 pKa = 3.82 VTTSSSPVLGPRR96 pKa = 11.84 GNFNFSCEE104 pKa = 4.16 GFDD107 pKa = 5.08 KK108 pKa = 10.72 SQCIIKK114 pKa = 10.43 SVSDD118 pKa = 4.18 FNAHH122 pKa = 4.1 YY123 pKa = 10.17 QIRR126 pKa = 11.84 NLRR129 pKa = 11.84 YY130 pKa = 9.01 VLACITDD137 pKa = 3.65 SEE139 pKa = 5.25 KK140 pKa = 10.85 IFDD143 pKa = 3.74 MCLFDD148 pKa = 5.98 SDD150 pKa = 3.23 IKK152 pKa = 11.04 RR153 pKa = 11.84 RR154 pKa = 11.84 DD155 pKa = 3.6 FLKK158 pKa = 10.85 VPVVPVLKK166 pKa = 10.8 LEE168 pKa = 4.2 NKK170 pKa = 9.7 RR171 pKa = 11.84 VLEE174 pKa = 4.18 VGSKK178 pKa = 10.11 FFFVDD183 pKa = 3.56 SSNDD187 pKa = 3.49 PVPIDD192 pKa = 3.99 PNAGMVNATISRR204 pKa = 11.84 LSVRR208 pKa = 11.84 LSGDD212 pKa = 3.55 CVITKK217 pKa = 10.24 VSMSSPYY224 pKa = 9.25 MIKK227 pKa = 10.1 IKK229 pKa = 9.42 TAEE232 pKa = 4.2 NIGFAVKK239 pKa = 10.27 DD240 pKa = 3.73 VRR242 pKa = 11.84 DD243 pKa = 3.77 SQAADD248 pKa = 3.41 LQTASGDD255 pKa = 3.11 RR256 pKa = 11.84 SVYY259 pKa = 10.23 FKK261 pKa = 11.19 NSEE264 pKa = 3.82 LDD266 pKa = 3.44 GNHH269 pKa = 6.25 FFLCGDD275 pKa = 3.53 KK276 pKa = 10.96 SSLISKK282 pKa = 9.79 VDD284 pKa = 3.42 VPVRR288 pKa = 11.84 NCVSKK293 pKa = 11.27 YY294 pKa = 10.19 ADD296 pKa = 3.58 EE297 pKa = 4.34 PKK299 pKa = 10.65 KK300 pKa = 10.79 IFFCANFSFFKK311 pKa = 10.32 WIFTLLVIAFPVTWLLWKK329 pKa = 9.11 TKK331 pKa = 9.32 NAFMVWYY338 pKa = 9.11 DD339 pKa = 3.36 IIGILTYY346 pKa = 10.33 PILVLINYY354 pKa = 7.15 FWVYY358 pKa = 10.02 FPFRR362 pKa = 11.84 CKK364 pKa = 10.65 VCGNLSFLTHH374 pKa = 6.35 TCSKK378 pKa = 10.92 LCVCNQCEE386 pKa = 3.96 PSKK389 pKa = 10.84 EE390 pKa = 4.13 HH391 pKa = 7.2 AKK393 pKa = 10.16 EE394 pKa = 4.17 CYY396 pKa = 9.82 LFSKK400 pKa = 9.42 DD401 pKa = 3.43 TQEE404 pKa = 3.72 WRR406 pKa = 11.84 SLSLIDD412 pKa = 4.66 HH413 pKa = 6.48 FQFTVNTKK421 pKa = 9.54 ISSEE425 pKa = 3.9 FLVFLTKK432 pKa = 9.96 MLIAAVLISYY442 pKa = 9.73 IPSSMALSMQKK453 pKa = 9.67 NVCVEE458 pKa = 3.89 RR459 pKa = 11.84 CYY461 pKa = 10.87 YY462 pKa = 10.92 SKK464 pKa = 11.01 DD465 pKa = 3.53 LEE467 pKa = 4.32 RR468 pKa = 11.84 LTTDD472 pKa = 3.03 KK473 pKa = 11.34 DD474 pKa = 3.76 GSSRR478 pKa = 11.84 NAIDD482 pKa = 3.65 TCDD485 pKa = 3.84 CSIGDD490 pKa = 5.28 LITEE494 pKa = 4.43 TVYY497 pKa = 10.87 RR498 pKa = 11.84 GGTPISRR505 pKa = 11.84 ATTKK509 pKa = 10.47 NDD511 pKa = 3.24 CVLSSSACLTSSNQAKK527 pKa = 10.4 NLFACRR533 pKa = 11.84 NGCNALTTLEE543 pKa = 4.8 KK544 pKa = 10.44 IPTVKK549 pKa = 10.53 YY550 pKa = 8.6 SSNYY554 pKa = 9.85 RR555 pKa = 11.84 GIEE558 pKa = 3.73 YY559 pKa = 10.45 SGNLTVLKK567 pKa = 10.16 IANRR571 pKa = 11.84 LRR573 pKa = 11.84 KK574 pKa = 10.13 GFVDD578 pKa = 3.45 NQSEE582 pKa = 4.35 VRR584 pKa = 11.84 NLEE587 pKa = 4.25 SKK589 pKa = 10.26 VSKK592 pKa = 10.66 DD593 pKa = 3.21 LLYY596 pKa = 10.86 FKK598 pKa = 10.3 NLKK601 pKa = 9.93 VDD603 pKa = 4.54 DD604 pKa = 4.48 VPPSNLMPRR613 pKa = 11.84 QSLVFSTEE621 pKa = 3.11 VDD623 pKa = 3.03 GKK625 pKa = 10.21 YY626 pKa = 9.98 RR627 pKa = 11.84 YY628 pKa = 9.71 LIEE631 pKa = 4.11 MDD633 pKa = 3.17 IKK635 pKa = 11.02 KK636 pKa = 8.58 EE637 pKa = 4.09 TGSVFLLNDD646 pKa = 4.06 DD647 pKa = 4.25 SSHH650 pKa = 7.44 VPMEE654 pKa = 3.82 FIIYY658 pKa = 8.5 VKK660 pKa = 10.73 NVGVDD665 pKa = 3.19 YY666 pKa = 10.61 DD667 pKa = 3.5 IKK669 pKa = 11.09 YY670 pKa = 10.11 KK671 pKa = 10.97 YY672 pKa = 9.3 STAKK676 pKa = 10.7 VEE678 pKa = 4.28 TTVTDD683 pKa = 4.17 FLSTCTGTCEE693 pKa = 4.26 DD694 pKa = 5.15 CKK696 pKa = 9.7 TQKK699 pKa = 10.52 PLTGRR704 pKa = 11.84 HH705 pKa = 5.9 DD706 pKa = 3.77 FCVQPTSWWGCEE718 pKa = 3.82 EE719 pKa = 4.55 LGCLAIDD726 pKa = 3.76 EE727 pKa = 4.6 GAICGHH733 pKa = 5.89 CTNVFDD739 pKa = 6.71 LSTLVNVYY747 pKa = 9.6 QVVQSHH753 pKa = 5.55 VAAEE757 pKa = 3.97 ICIKK761 pKa = 10.52 SVNGYY766 pKa = 9.05 DD767 pKa = 4.32 CRR769 pKa = 11.84 KK770 pKa = 8.75 HH771 pKa = 5.23 TDD773 pKa = 3.12 KK774 pKa = 11.38 VPIQTDD780 pKa = 4.42 YY781 pKa = 10.94 YY782 pKa = 9.64 QLDD785 pKa = 3.82 MTVDD789 pKa = 3.35 LHH791 pKa = 7.06 NDD793 pKa = 3.14 YY794 pKa = 10.9 MSTDD798 pKa = 3.08 KK799 pKa = 11.27 LFAVNKK805 pKa = 6.17 QQKK808 pKa = 9.14 VLTGSISDD816 pKa = 4.26 LGDD819 pKa = 3.6 FSSSAFGHH827 pKa = 5.27 PQINIDD833 pKa = 3.99 GKK835 pKa = 9.66 PLAVPATLSRR845 pKa = 11.84 DD846 pKa = 3.48 QFSWSCSAVGAKK858 pKa = 9.33 SVNIRR863 pKa = 11.84 QCGLFSYY870 pKa = 11.14 NMIYY874 pKa = 10.98 ALTQSRR880 pKa = 11.84 DD881 pKa = 3.37 FAVLSEE887 pKa = 4.67 SEE889 pKa = 4.17 NKK891 pKa = 10.6 LYY893 pKa = 7.53 MTKK896 pKa = 10.82 DD897 pKa = 3.41 FLVGKK902 pKa = 9.42 LKK904 pKa = 11.0 VVVDD908 pKa = 3.83 MPKK911 pKa = 10.84 EE912 pKa = 4.09 MFKK915 pKa = 10.83 KK916 pKa = 10.2 PPSKK920 pKa = 10.17 PAISEE925 pKa = 4.31 TKK927 pKa = 10.07 VNCSGCFKK935 pKa = 10.51 CAMGLEE941 pKa = 4.48 CDD943 pKa = 4.75 LEE945 pKa = 4.48 YY946 pKa = 10.84 TSDD949 pKa = 3.42 TTFSSRR955 pKa = 11.84 LEE957 pKa = 3.78 IEE959 pKa = 4.27 GCSFKK964 pKa = 10.78 SDD966 pKa = 3.44 QLGSYY971 pKa = 9.83 IGPNKK976 pKa = 10.22 KK977 pKa = 9.96 KK978 pKa = 10.18 IKK980 pKa = 9.73 AYY982 pKa = 9.98 CSEE985 pKa = 4.33 SIKK988 pKa = 10.94 DD989 pKa = 3.67 KK990 pKa = 10.19 NIKK993 pKa = 8.72 MVAEE997 pKa = 5.09 DD998 pKa = 4.68 DD999 pKa = 4.5 DD1000 pKa = 4.21 EE1001 pKa = 6.06 LSIEE1005 pKa = 4.17 LKK1007 pKa = 9.95 IDD1009 pKa = 3.3 QVDD1012 pKa = 3.78 VVEE1015 pKa = 4.58 QDD1017 pKa = 3.47 TIINYY1022 pKa = 9.43 DD1023 pKa = 3.75 DD1024 pKa = 4.93 KK1025 pKa = 11.42 SAHH1028 pKa = 7.14 DD1029 pKa = 4.2 EE1030 pKa = 4.52 NIHH1033 pKa = 6.92 HH1034 pKa = 7.19 SDD1036 pKa = 3.19 TGLATLWDD1044 pKa = 4.21 WIKK1047 pKa = 11.44 APFNWVASFFGNFFDD1062 pKa = 3.5 IVRR1065 pKa = 11.84 VILVIIAICVGIYY1078 pKa = 9.67 IISYY1082 pKa = 9.4 IYY1084 pKa = 10.61 SLSFSYY1090 pKa = 11.04 YY1091 pKa = 9.47 KK1092 pKa = 10.36 EE1093 pKa = 3.75 KK1094 pKa = 10.6 RR1095 pKa = 11.84 NKK1097 pKa = 10.17 KK1098 pKa = 9.6 IEE1100 pKa = 4.05 FDD1102 pKa = 3.79 LEE1104 pKa = 4.18 EE1105 pKa = 4.29 VSQSLLKK1112 pKa = 9.87 MSKK1115 pKa = 9.84 RR1116 pKa = 11.84 DD1117 pKa = 3.3 GEE1119 pKa = 4.38 RR1120 pKa = 11.84 RR1121 pKa = 11.84 RR1122 pKa = 11.84 KK1123 pKa = 9.97 SPPKK1127 pKa = 8.97 TFEE1130 pKa = 4.0 FPLEE1134 pKa = 4.05 FF1135 pKa = 5.44
Molecular weight: 128.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.805
IPC2_protein 5.944
IPC_protein 5.97
Toseland 6.097
ProMoST 6.351
Dawson 6.287
Bjellqvist 6.313
Wikipedia 6.287
Rodwell 6.275
Grimsley 6.148
Solomon 6.287
Lehninger 6.275
Nozaki 6.576
DTASelect 6.722
Thurlkill 6.722
EMBOSS 6.693
Sillero 6.664
Patrickios 3.91
IPC_peptide 6.3
IPC2_peptide 6.532
IPC2.peptide.svr19 6.592
Protein with the highest isoelectric point:
>tr|A4GW61|A4GW61_9VIRU Nucleoprotein OS=Polygonum ringspot tospovirus OX=430606 GN=N PE=3 SV=1
MM1 pKa = 7.18 STTGLSKK8 pKa = 11.15 EE9 pKa = 4.16 NVEE12 pKa = 4.29 KK13 pKa = 10.98 LLAGGDD19 pKa = 3.34 ADD21 pKa = 4.12 VEE23 pKa = 4.3 IEE25 pKa = 4.13 AEE27 pKa = 4.11 EE28 pKa = 3.91 TAGFNFKK35 pKa = 10.67 QFVTDD40 pKa = 3.55 NSKK43 pKa = 10.26 MKK45 pKa = 8.56 MTFNNGFTILRR56 pKa = 11.84 NRR58 pKa = 11.84 AAIYY62 pKa = 10.75 KK63 pKa = 8.15 MVKK66 pKa = 9.76 AGHH69 pKa = 6.32 FKK71 pKa = 10.68 FQGKK75 pKa = 9.99 SIVVPSSTSSPGQDD89 pKa = 2.27 DD90 pKa = 3.56 WTFRR94 pKa = 11.84 RR95 pKa = 11.84 LEE97 pKa = 3.88 GFIRR101 pKa = 11.84 AKK103 pKa = 10.26 MFMEE107 pKa = 5.64 LINMEE112 pKa = 4.33 NAVEE116 pKa = 3.92 QQKK119 pKa = 9.76 MYY121 pKa = 10.96 EE122 pKa = 4.29 KK123 pKa = 10.53 LCEE126 pKa = 3.95 LPMVNAYY133 pKa = 9.31 GLKK136 pKa = 9.9 PSSKK140 pKa = 10.05 FDD142 pKa = 3.26 PTTARR147 pKa = 11.84 VVLTLGGPLPLMASLDD163 pKa = 3.76 KK164 pKa = 10.58 FAAAAFPLAYY174 pKa = 9.6 FQNVKK179 pKa = 10.56 KK180 pKa = 10.21 EE181 pKa = 3.83 ALGINKK187 pKa = 9.37 FSTYY191 pKa = 9.74 EE192 pKa = 3.86 QLCKK196 pKa = 9.76 IARR199 pKa = 11.84 VMATKK204 pKa = 10.54 EE205 pKa = 3.97 FTFTGASKK213 pKa = 10.88 AIFEE217 pKa = 4.32 EE218 pKa = 4.76 TIKK221 pKa = 10.63 ILNDD225 pKa = 3.62 CTPGTAGAASLNKK238 pKa = 9.94 FNEE241 pKa = 4.15 QIKK244 pKa = 10.54 ALEE247 pKa = 4.02 AVFGKK252 pKa = 10.36 IVDD255 pKa = 4.18 DD256 pKa = 4.45 NNAGSSKK263 pKa = 9.96 PRR265 pKa = 11.84 PASKK269 pKa = 10.41 KK270 pKa = 9.06 NQGFF274 pKa = 3.44
Molecular weight: 30.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.622
IPC2_protein 8.419
IPC_protein 8.317
Toseland 9.253
ProMoST 8.887
Dawson 9.458
Bjellqvist 9.165
Wikipedia 9.545
Rodwell 9.94
Grimsley 9.472
Solomon 9.516
Lehninger 9.502
Nozaki 9.414
DTASelect 9.092
Thurlkill 9.341
EMBOSS 9.663
Sillero 9.443
Patrickios 5.664
IPC_peptide 9.516
IPC2_peptide 7.644
IPC2.peptide.svr19 7.848
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5036
274
2876
1007.2
114.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.527 ± 1.034
2.264 ± 0.454
6.215 ± 0.399
6.334 ± 0.454
5.242 ± 0.301
4.21 ± 0.461
1.728 ± 0.185
7.168 ± 0.316
8.817 ± 0.324
9.015 ± 0.717
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.415 ± 0.579
6.056 ± 0.447
2.859 ± 0.405
2.562 ± 0.081
3.594 ± 0.179
9.373 ± 0.484
5.977 ± 0.082
5.997 ± 0.629
0.774 ± 0.154
3.872 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here