Gordonia iterans
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3450 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0KG08|A0A2S0KG08_9ACTN Shikimate kinase OS=Gordonia iterans OX=1004901 GN=aroK PE=3 SV=1
MM1 pKa = 7.64 SRR3 pKa = 11.84 FLQFYY8 pKa = 10.09 FSLNDD13 pKa = 3.47 SGSVAEE19 pKa = 4.21 QQMTEE24 pKa = 3.8 AVRR27 pKa = 11.84 LVLSADD33 pKa = 3.75 PDD35 pKa = 4.28 DD36 pKa = 5.72 DD37 pKa = 3.93 PVEE40 pKa = 4.51 AGGPVDD46 pKa = 3.43 EE47 pKa = 5.15 ARR49 pKa = 11.84 VEE51 pKa = 4.39 EE52 pKa = 4.48 IMSVAHH58 pKa = 6.68 SAAQDD63 pKa = 3.46 GHH65 pKa = 6.61 PLPLEE70 pKa = 4.1 EE71 pKa = 5.16 RR72 pKa = 11.84 SDD74 pKa = 3.76 VSVDD78 pKa = 3.72 LGPGATVSAFSDD90 pKa = 3.84 SLPPEE95 pKa = 4.69 CPQCHH100 pKa = 6.08 EE101 pKa = 5.17 LLSDD105 pKa = 4.01 WDD107 pKa = 3.85 PQEE110 pKa = 3.66 WWDD113 pKa = 4.49 GGDD116 pKa = 3.85 EE117 pKa = 4.48 PEE119 pKa = 4.6 EE120 pKa = 4.03 QCPSCGFTALVGDD133 pKa = 4.45 WLIQSTPYY141 pKa = 11.11 ARR143 pKa = 11.84 TEE145 pKa = 4.09 CGLVLVDD152 pKa = 3.65 WPSLEE157 pKa = 4.16 EE158 pKa = 4.19 YY159 pKa = 10.9 GPEE162 pKa = 3.85 LHH164 pKa = 7.39 DD165 pKa = 4.83 ALLSTVGSRR174 pKa = 11.84 PRR176 pKa = 11.84 YY177 pKa = 9.11 VSGHH181 pKa = 4.1 VV182 pKa = 3.02
Molecular weight: 19.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.643
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.126
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.961
Patrickios 1.1
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A2S0KKF0|A0A2S0KKF0_9ACTN Amidohydro-rel domain-containing protein OS=Gordonia iterans OX=1004901 GN=C6V83_08905 PE=4 SV=1
MM1 pKa = 7.32 PRR3 pKa = 11.84 KK4 pKa = 9.11 PRR6 pKa = 11.84 TVRR9 pKa = 11.84 SPSPVRR15 pKa = 11.84 RR16 pKa = 11.84 ARR18 pKa = 11.84 RR19 pKa = 11.84 FRR21 pKa = 11.84 RR22 pKa = 11.84 PRR24 pKa = 11.84 TRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 PPPSKK33 pKa = 10.29 RR34 pKa = 11.84 PLRR37 pKa = 11.84 RR38 pKa = 11.84 PPRR41 pKa = 11.84 GVRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 TRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 LRR52 pKa = 11.84 PARR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 PTGCSRR63 pKa = 11.84 APSS66 pKa = 3.42
Molecular weight: 8.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.647
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.354
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.275
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3450
0
3450
1168669
28
10134
338.7
36.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.323 ± 0.055
0.725 ± 0.014
6.553 ± 0.033
5.547 ± 0.032
2.937 ± 0.022
9.136 ± 0.038
2.015 ± 0.02
4.104 ± 0.03
2.139 ± 0.033
9.842 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.898 ± 0.018
1.97 ± 0.023
5.818 ± 0.029
2.755 ± 0.022
7.506 ± 0.046
5.36 ± 0.027
5.96 ± 0.03
8.945 ± 0.049
1.416 ± 0.019
2.052 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here