Cupriavidus sp. UYMSc13B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; unclassified Cupriavidus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A439EWM3|A0A439EWM3_9BURK Quinoprotein dehydrogenase-associated SoxYZ-like carrier OS=Cupriavidus sp. UYMSc13B OX=1774746 GN=AU476_38075 PE=4 SV=1
MM1 pKa = 7.05EE2 pKa = 5.21AAVAYY7 pKa = 6.74KK8 pKa = 9.43TWVCVICGWVYY19 pKa = 11.1DD20 pKa = 4.71EE21 pKa = 4.29EE22 pKa = 4.75QGWPDD27 pKa = 4.15DD28 pKa = 4.55GIAPGTRR35 pKa = 11.84WEE37 pKa = 5.63DD38 pKa = 3.21IPEE41 pKa = 3.98DD42 pKa = 3.85WRR44 pKa = 11.84CPEE47 pKa = 4.22CDD49 pKa = 3.03VGKK52 pKa = 10.91AEE54 pKa = 4.39FAMIEE59 pKa = 4.1LL60 pKa = 4.4

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A439EY05|A0A439EY05_9BURK Fis family transcriptional regulator OS=Cupriavidus sp. UYMSc13B OX=1774746 GN=AU476_32010 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3607

0

3607

782477

29

1552

216.9

23.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.45 ± 0.07

1.116 ± 0.015

5.186 ± 0.034

5.227 ± 0.044

3.581 ± 0.029

8.099 ± 0.043

2.403 ± 0.024

4.552 ± 0.032

3.16 ± 0.039

10.205 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.02

2.723 ± 0.03

5.134 ± 0.035

3.845 ± 0.028

7.313 ± 0.045

5.571 ± 0.038

5.299 ± 0.034

7.567 ± 0.036

1.462 ± 0.019

2.449 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski