Streptococcus satellite phage Javan473

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZU81|A0A4D5ZU81_9VIRU ArpU family encoded transcriptional regulator OS=Streptococcus satellite phage Javan473 OX=2558718 GN=JavanS473_0021 PE=4 SV=1
MM1 pKa = 6.36TTEE4 pKa = 4.55EE5 pKa = 4.36YY6 pKa = 10.33FKK8 pKa = 11.24KK9 pKa = 10.6LLEE12 pKa = 4.78DD13 pKa = 4.07SEE15 pKa = 4.43KK16 pKa = 10.95QPTNWLSDD24 pKa = 3.11RR25 pKa = 11.84VLDD28 pKa = 5.06KK29 pKa = 10.89NIKK32 pKa = 9.2MLDD35 pKa = 3.47EE36 pKa = 5.39DD37 pKa = 5.92IEE39 pKa = 4.65AEE41 pKa = 3.93WDD43 pKa = 3.67DD44 pKa = 4.9FPLFTKK50 pKa = 10.43KK51 pKa = 10.31IFINTTQRR59 pKa = 11.84DD60 pKa = 4.03YY61 pKa = 11.76NKK63 pKa = 10.39ADD65 pKa = 3.31AVVKK69 pKa = 9.84FLEE72 pKa = 4.69SIPIEE77 pKa = 4.07MEE79 pKa = 3.85YY80 pKa = 10.97KK81 pKa = 10.18KK82 pKa = 10.89GLNIVIQALNEE93 pKa = 4.18LKK95 pKa = 10.65DD96 pKa = 3.62SLKK99 pKa = 10.67NQVLGVIDD107 pKa = 3.94NQILFPNDD115 pKa = 3.42KK116 pKa = 10.21EE117 pKa = 4.26DD118 pKa = 3.53TKK120 pKa = 11.66

Molecular weight: 14.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPR9|A0A4D5ZPR9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan473 OX=2558718 GN=JavanS473_0012 PE=4 SV=1
MM1 pKa = 7.57SGGGAYY7 pKa = 9.43VCKK10 pKa = 10.2SQKK13 pKa = 9.01PKK15 pKa = 9.91EE16 pKa = 4.06RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84QGLSVYY25 pKa = 7.65EE26 pKa = 4.27TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89NKK47 pKa = 10.56

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3550

47

562

161.4

18.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.662 ± 0.371

0.817 ± 0.271

5.577 ± 0.378

7.803 ± 0.576

3.718 ± 0.256

5.014 ± 0.376

1.887 ± 0.309

6.873 ± 0.449

9.549 ± 0.473

10.282 ± 0.459

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.203

5.07 ± 0.325

3.099 ± 0.447

4.592 ± 0.269

5.239 ± 0.36

5.408 ± 0.323

6.31 ± 0.354

5.155 ± 0.433

1.07 ± 0.186

4.648 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski