Brevundimonas sp. Root1423

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7SUF4|A0A0Q7SUF4_9CAUL TetR family transcriptional regulator OS=Brevundimonas sp. Root1423 OX=1736462 GN=ASD25_19465 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.57SLFLATAAVAAFAAAPALAQEE23 pKa = 4.59PVGSVGLTYY32 pKa = 10.61SDD34 pKa = 3.54SQIEE38 pKa = 4.25FGGLDD43 pKa = 3.68AEE45 pKa = 4.45SDD47 pKa = 3.96SYY49 pKa = 12.18ALDD52 pKa = 3.57GVVAVPAFGRR62 pKa = 11.84WTFTAAANASQTDD75 pKa = 3.77DD76 pKa = 4.29GADD79 pKa = 3.76DD80 pKa = 3.89DD81 pKa = 4.58TAFTGTAHH89 pKa = 7.17LTTLAGSDD97 pKa = 3.48LRR99 pKa = 11.84IGGFVGASDD108 pKa = 4.28YY109 pKa = 11.77ADD111 pKa = 3.6EE112 pKa = 4.4TALTAGAEE120 pKa = 4.21VQKK123 pKa = 10.89YY124 pKa = 9.94LGSATLTGVVAYY136 pKa = 8.63TSADD140 pKa = 3.73DD141 pKa = 4.1SDD143 pKa = 3.51VDD145 pKa = 3.51AWSVGADD152 pKa = 2.86AAFYY156 pKa = 11.02VMPNLRR162 pKa = 11.84LNAGIGYY169 pKa = 7.57VTADD173 pKa = 3.13AAGFDD178 pKa = 4.36VDD180 pKa = 4.01GMTYY184 pKa = 10.62GAGAEE189 pKa = 4.05YY190 pKa = 10.42QIGGTPFSVTAGYY203 pKa = 10.05SHH205 pKa = 7.68ADD207 pKa = 3.4VEE209 pKa = 5.23DD210 pKa = 4.0IDD212 pKa = 4.17VDD214 pKa = 4.18TVSVGLRR221 pKa = 11.84YY222 pKa = 10.26SFGGGLQARR231 pKa = 11.84DD232 pKa = 3.24RR233 pKa = 11.84AGAALPGSGVMGLLGALL250 pKa = 3.82

Molecular weight: 25.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7RU80|A0A0Q7RU80_9CAUL Succinyl-CoA--3-ketoacid-CoA transferase OS=Brevundimonas sp. Root1423 OX=1736462 GN=ASD25_13320 PE=3 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84KK3 pKa = 9.04RR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 4.9GFRR9 pKa = 11.84SRR11 pKa = 11.84MATKK15 pKa = 10.2NGQKK19 pKa = 9.53IVARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84AKK27 pKa = 9.77GRR29 pKa = 11.84KK30 pKa = 9.04RR31 pKa = 11.84LTAA34 pKa = 4.18

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5246

0

5246

1553365

34

4124

296.1

31.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.049 ± 0.055

0.703 ± 0.01

6.027 ± 0.032

5.508 ± 0.033

3.445 ± 0.021

9.314 ± 0.053

1.741 ± 0.017

4.325 ± 0.027

2.46 ± 0.033

9.813 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.019

2.326 ± 0.032

5.547 ± 0.034

3.006 ± 0.021

7.654 ± 0.043

5.101 ± 0.033

5.489 ± 0.041

7.673 ± 0.029

1.499 ± 0.015

1.981 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski