Propionibacterium cyclohexanicum
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2509 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9QPL0|A0A1H9QPL0_9ACTN Phosphatidylinositol alpha-mannosyltransferase OS=Propionibacterium cyclohexanicum OX=64702 GN=SAMN05443377_1048 PE=4 SV=1
MM1 pKa = 7.44 TEE3 pKa = 4.0 VPSDD7 pKa = 3.44 LLYY10 pKa = 10.97 SRR12 pKa = 11.84 EE13 pKa = 4.25 HH14 pKa = 5.05 EE15 pKa = 4.18 WVRR18 pKa = 11.84 VEE20 pKa = 3.96 GATARR25 pKa = 11.84 VGVTEE30 pKa = 3.88 YY31 pKa = 11.06 AARR34 pKa = 11.84 EE35 pKa = 4.08 LGDD38 pKa = 3.42 IVYY41 pKa = 10.33 VSAPTVGAQVVAGDD55 pKa = 3.7 PCGEE59 pKa = 4.13 LEE61 pKa = 4.31 STKK64 pKa = 10.73 SVSDD68 pKa = 3.68 LFAPATGIVSAVNEE82 pKa = 4.38 ALDD85 pKa = 3.93 EE86 pKa = 4.42 SPEE89 pKa = 4.31 TVNAAPYY96 pKa = 9.24 EE97 pKa = 4.2 EE98 pKa = 4.56 GWLFEE103 pKa = 4.64 VEE105 pKa = 4.5 LADD108 pKa = 5.41 DD109 pKa = 4.59 PGVDD113 pKa = 3.51 EE114 pKa = 5.76 LLDD117 pKa = 3.73 AEE119 pKa = 5.26 AYY121 pKa = 9.85 QEE123 pKa = 5.22 LIGEE127 pKa = 4.3 QQ128 pKa = 3.34
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.719
IPC_protein 3.643
Toseland 3.478
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.503
Rodwell 3.49
Grimsley 3.389
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.846
Thurlkill 3.503
EMBOSS 3.516
Sillero 3.757
Patrickios 1.786
IPC_peptide 3.592
IPC2_peptide 3.745
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A1H9TSU9|A0A1H9TSU9_9ACTN Asparagine synthase (Glutamine-hydrolysing) OS=Propionibacterium cyclohexanicum OX=64702 GN=SAMN05443377_1282 PE=3 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.95 RR4 pKa = 11.84 TYY6 pKa = 9.93 QPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 THH17 pKa = 5.8 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSSRR26 pKa = 11.84 AGRR29 pKa = 11.84 GILSARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.98 GRR40 pKa = 11.84 TEE42 pKa = 3.99 LSAA45 pKa = 4.86
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.364
IPC2_protein 10.921
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 12.998
Rodwell 12.106
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.842
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2509
0
2509
833824
25
2337
332.3
35.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.588 ± 0.06
0.893 ± 0.015
5.706 ± 0.045
5.324 ± 0.044
2.924 ± 0.029
8.927 ± 0.039
2.356 ± 0.025
4.393 ± 0.036
2.127 ± 0.035
10.249 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.083 ± 0.02
2.103 ± 0.023
5.564 ± 0.038
3.456 ± 0.027
7.425 ± 0.053
6.126 ± 0.037
5.788 ± 0.034
8.472 ± 0.047
1.518 ± 0.021
1.978 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here