Termite gut associated microvirus 2
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3ISQ8|A0A3G3ISQ8_9VIRU Uncharacterized protein OS=Termite gut associated microvirus 2 OX=2486189 PE=4 SV=1
MM1 pKa = 7.44 FPTYY5 pKa = 10.29 NDD7 pKa = 3.39 KK8 pKa = 10.84 EE9 pKa = 4.16 GRR11 pKa = 11.84 EE12 pKa = 4.2 ILDD15 pKa = 3.72 EE16 pKa = 4.51 TPVEE20 pKa = 4.02 APLRR24 pKa = 11.84 MRR26 pKa = 11.84 KK27 pKa = 8.87 PLTLQEE33 pKa = 4.14 QVDD36 pKa = 4.51 LFLGRR41 pKa = 11.84 ALYY44 pKa = 10.41 SSHH47 pKa = 6.62 EE48 pKa = 4.2 PSDD51 pKa = 3.65 TLDD54 pKa = 4.61 DD55 pKa = 5.52 LDD57 pKa = 5.35 VDD59 pKa = 5.42 DD60 pKa = 6.59 DD61 pKa = 4.19 GQAYY65 pKa = 10.56 DD66 pKa = 4.03 DD67 pKa = 4.42 FPYY70 pKa = 10.49 EE71 pKa = 3.92 IEE73 pKa = 4.49 PPVQVRR79 pKa = 11.84 KK80 pKa = 10.25 KK81 pKa = 9.44 KK82 pKa = 10.94 APVPPPAPGGEE93 pKa = 4.17 GGTAEE98 pKa = 4.67 KK99 pKa = 9.9 PAEE102 pKa = 4.28 SSSDD106 pKa = 3.55 PKK108 pKa = 10.51 PASSS112 pKa = 3.45
Molecular weight: 12.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.438
IPC2_protein 4.266
IPC_protein 4.202
Toseland 4.012
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.024
Grimsley 3.923
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.469
Thurlkill 4.037
EMBOSS 4.088
Sillero 4.317
Patrickios 3.948
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.242
Protein with the highest isoelectric point:
>tr|A0A3G3ISR0|A0A3G3ISR0_9VIRU Replication initiator protein OS=Termite gut associated microvirus 2 OX=2486189 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.18 KK3 pKa = 9.33 SSSRR7 pKa = 11.84 KK8 pKa = 7.46 RR9 pKa = 11.84 HH10 pKa = 5.17 FRR12 pKa = 11.84 PSRR15 pKa = 11.84 PQRR18 pKa = 11.84 DD19 pKa = 3.86 VLHH22 pKa = 6.5 SPRR25 pKa = 11.84 LTRR28 pKa = 11.84 LLRR31 pKa = 11.84 TQNRR35 pKa = 11.84 YY36 pKa = 9.67 YY37 pKa = 10.75 FSDD40 pKa = 3.25 SLGPDD45 pKa = 3.21 LPHH48 pKa = 7.1 RR49 pKa = 11.84 RR50 pKa = 11.84 IEE52 pKa = 3.94 VRR54 pKa = 11.84 KK55 pKa = 8.55 PLSRR59 pKa = 11.84 LPSDD63 pKa = 3.74 FRR65 pKa = 11.84 SVRR68 pKa = 11.84 HH69 pKa = 6.05 SPTPTPSDD77 pKa = 4.1 LLSVMAPDD85 pKa = 3.61 LTKK88 pKa = 10.5 PRR90 pKa = 11.84 TMPDD94 pKa = 2.69 ILRR97 pKa = 11.84 HH98 pKa = 5.41 IICRR102 pKa = 11.84 GRR104 pKa = 11.84 KK105 pKa = 7.48 VRR107 pKa = 11.84 RR108 pKa = 11.84 QVLHH112 pKa = 6.64 ALDD115 pKa = 4.17 LVGHH119 pKa = 7.03 KK120 pKa = 10.5 GQGGSRR126 pKa = 11.84 DD127 pKa = 3.46 RR128 pKa = 11.84 RR129 pKa = 11.84 ITPSSEE135 pKa = 3.65 LRR137 pKa = 11.84 CC138 pKa = 4.06
Molecular weight: 16.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.152
IPC2_protein 10.16
IPC_protein 11.608
Toseland 11.769
ProMoST 12.252
Dawson 11.784
Bjellqvist 11.769
Wikipedia 12.237
Rodwell 11.506
Grimsley 11.813
Solomon 12.266
Lehninger 12.164
Nozaki 11.769
DTASelect 11.754
Thurlkill 11.769
EMBOSS 12.266
Sillero 11.769
Patrickios 11.228
IPC_peptide 12.266
IPC2_peptide 11.242
IPC2.peptide.svr19 9.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1378
112
535
275.6
31.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.459 ± 1.947
0.871 ± 0.499
6.023 ± 0.686
4.136 ± 0.727
4.499 ± 0.653
7.039 ± 0.813
3.048 ± 0.532
4.499 ± 0.423
3.266 ± 0.528
8.781 ± 0.548
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.669 ± 0.143
3.919 ± 0.722
7.983 ± 0.895
4.79 ± 0.979
7.91 ± 1.382
8.708 ± 0.59
5.08 ± 0.855
5.515 ± 0.494
1.451 ± 0.352
4.354 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here