Mycoplasma glycophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449AV37|A0A449AV37_9MOLU Spermidine/putrescine ABC transporter membrane protein OS=Mycoplasma glycophilum OX=171285 GN=NCTC10194_00388 PE=4 SV=1
MM1 pKa = 7.33NFDD4 pKa = 4.51KK5 pKa = 11.01EE6 pKa = 5.31KK7 pKa = 10.07ILNWFKK13 pKa = 11.33NQDD16 pKa = 3.27KK17 pKa = 10.96DD18 pKa = 3.92SLVEE22 pKa = 3.83QIYY25 pKa = 9.9EE26 pKa = 4.0KK27 pKa = 11.4VMLYY31 pKa = 10.56EE32 pKa = 4.24DD33 pKa = 3.43WPYY36 pKa = 11.14INDD39 pKa = 3.38VFYY42 pKa = 10.84DD43 pKa = 4.04CPLYY47 pKa = 10.79DD48 pKa = 4.72YY49 pKa = 11.05IDD51 pKa = 4.01AFEE54 pKa = 4.16KK55 pKa = 10.22TIQKK59 pKa = 10.62EE60 pKa = 4.23NFNSLGEE67 pKa = 4.19CIYY70 pKa = 10.97YY71 pKa = 9.71IEE73 pKa = 4.77CEE75 pKa = 4.09KK76 pKa = 10.85LPSIAEE82 pKa = 3.83THH84 pKa = 6.41INTKK88 pKa = 9.79EE89 pKa = 3.76NQLAEE94 pKa = 4.17KK95 pKa = 7.82TTEE98 pKa = 4.19KK99 pKa = 10.49IKK101 pKa = 10.71FLIDD105 pKa = 3.05KK106 pKa = 9.85DD107 pKa = 3.33PWYY110 pKa = 10.83FEE112 pKa = 4.25YY113 pKa = 10.31IKK115 pKa = 10.24EE116 pKa = 4.11KK117 pKa = 10.15TSIYY121 pKa = 10.59DD122 pKa = 3.33VLKK125 pKa = 10.66AAEE128 pKa = 4.28KK129 pKa = 9.88TLINYY134 pKa = 7.65FLYY137 pKa = 10.6HH138 pKa = 6.75SNNTFEE144 pKa = 5.64NILEE148 pKa = 4.31NEE150 pKa = 4.37LEE152 pKa = 4.14LEE154 pKa = 4.46EE155 pKa = 4.93DD156 pKa = 4.16NEE158 pKa = 4.4MTLL161 pKa = 4.31

Molecular weight: 19.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449AUP7|A0A449AUP7_9MOLU Uncharacterized protein OS=Mycoplasma glycophilum OX=171285 GN=NCTC10194_00204 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.97KK9 pKa = 10.37AKK11 pKa = 9.79RR12 pKa = 11.84QPKK15 pKa = 9.09FAVRR19 pKa = 11.84AYY21 pKa = 8.35TRR23 pKa = 11.84CEE25 pKa = 3.79LCGRR29 pKa = 11.84PHH31 pKa = 7.07AVLRR35 pKa = 11.84KK36 pKa = 9.32YY37 pKa = 9.94KK38 pKa = 10.01VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.85EE50 pKa = 4.47GKK52 pKa = 10.15IPGMKK57 pKa = 9.27KK58 pKa = 10.56ASWW61 pKa = 3.19

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

692

0

692

288362

36

6400

416.7

47.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.881 ± 0.129

0.381 ± 0.022

5.785 ± 0.129

7.538 ± 0.096

5.158 ± 0.139

4.367 ± 0.103

1.319 ± 0.034

8.67 ± 0.121

10.087 ± 0.116

9.735 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.586 ± 0.048

8.054 ± 0.153

2.615 ± 0.058

3.774 ± 0.066

2.92 ± 0.064

6.389 ± 0.075

5.355 ± 0.09

5.364 ± 0.087

0.93 ± 0.029

4.092 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski