Streptococcus phage Javan371
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A9P5|A0A4D6A9P5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan371 OX=2548132 GN=Javan371_0026 PE=4 SV=1
MM1 pKa = 8.06 SYY3 pKa = 10.54 EE4 pKa = 4.39 CSNCCKK10 pKa = 10.34 EE11 pKa = 4.0 IEE13 pKa = 4.24 DD14 pKa = 4.4 DD15 pKa = 4.26 FFVVQEE21 pKa = 3.99 NHH23 pKa = 6.9 VILALFNDD31 pKa = 4.23 VEE33 pKa = 4.52 NCFCSQQCVNDD44 pKa = 3.43 FLMIEE49 pKa = 4.28 SKK51 pKa = 10.88 YY52 pKa = 10.71 LSNGDD57 pKa = 3.17 IPYY60 pKa = 10.64 DD61 pKa = 3.79 EE62 pKa = 4.95 EE63 pKa = 4.37 AVEE66 pKa = 4.11
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.928
IPC2_protein 3.694
IPC_protein 3.579
Toseland 3.401
ProMoST 3.706
Dawson 3.567
Bjellqvist 3.808
Wikipedia 3.49
Rodwell 3.427
Grimsley 3.325
Solomon 3.528
Lehninger 3.478
Nozaki 3.706
DTASelect 3.821
Thurlkill 3.478
EMBOSS 3.503
Sillero 3.706
Patrickios 0.006
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A4D6A8H9|A0A4D6A8H9_9CAUD Single-stranded DNA-binding protein OS=Streptococcus phage Javan371 OX=2548132 GN=Javan371_0017 PE=3 SV=1
MM1 pKa = 6.31 TTIEE5 pKa = 4.9 IILAVAFVTYY15 pKa = 10.55 ILFSGFAIYY24 pKa = 10.87 VMRR27 pKa = 11.84 EE28 pKa = 3.66 VIVRR32 pKa = 11.84 QKK34 pKa = 11.6 AKK36 pKa = 8.93 MKK38 pKa = 9.62 HH39 pKa = 5.1 YY40 pKa = 10.68 KK41 pKa = 8.22 SAKK44 pKa = 6.91 YY45 pKa = 9.04 QRR47 pKa = 11.84 EE48 pKa = 3.87 MWNKK52 pKa = 9.34 RR53 pKa = 11.84 MSEE56 pKa = 3.68 IHH58 pKa = 5.82 QKK60 pKa = 9.09 RR61 pKa = 11.84 TVKK64 pKa = 10.46 GMSEE68 pKa = 3.95 LL69 pKa = 3.76
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.192
IPC2_protein 9.677
IPC_protein 9.794
Toseland 10.35
ProMoST 9.955
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.643
Rodwell 11.052
Grimsley 10.555
Solomon 10.526
Lehninger 10.511
Nozaki 10.306
DTASelect 10.131
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.409
Patrickios 10.804
IPC_peptide 10.526
IPC2_peptide 8.682
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
11758
40
1434
192.8
21.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.923 ± 0.599
0.485 ± 0.108
6.489 ± 0.292
8.318 ± 0.445
4.584 ± 0.299
6.268 ± 0.424
1.327 ± 0.126
6.472 ± 0.303
8.99 ± 0.325
8.496 ± 0.336
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.141
5.97 ± 0.239
2.917 ± 0.261
4.38 ± 0.302
3.793 ± 0.235
5.911 ± 0.313
5.843 ± 0.304
6.141 ± 0.299
1.038 ± 0.086
3.036 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here