Common midwife toad virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0VGL7|A0A0A0VGL7_9VIRU Uncharacterized protein OS=Common midwife toad virus OX=540070 GN=orf54L PE=4 SV=1
MM1 pKa = 7.31FALEE5 pKa = 4.36TYY7 pKa = 9.33KK8 pKa = 10.87CKK10 pKa = 10.52NSSMSFKK17 pKa = 10.59RR18 pKa = 11.84IMDD21 pKa = 3.65ATDD24 pKa = 2.87IFGDD28 pKa = 3.35ISDD31 pKa = 4.37IGSASSGDD39 pKa = 3.59EE40 pKa = 3.86AEE42 pKa = 4.69FEE44 pKa = 3.91HH45 pKa = 7.49DD46 pKa = 4.07EE47 pKa = 4.19NVEE50 pKa = 3.88RR51 pKa = 11.84AGYY54 pKa = 9.34EE55 pKa = 3.92EE56 pKa = 5.35PDD58 pKa = 3.63DD59 pKa = 5.44DD60 pKa = 5.45GVDD63 pKa = 3.78GVDD66 pKa = 3.83SVNQVEE72 pKa = 4.51AEE74 pKa = 4.06EE75 pKa = 3.88EE76 pKa = 3.84AAAVRR81 pKa = 11.84EE82 pKa = 4.31APSVVMPLDD91 pKa = 3.45MEE93 pKa = 4.76SYY95 pKa = 10.71SDD97 pKa = 3.96EE98 pKa = 4.32EE99 pKa = 4.31LAMMPLGAFASRR111 pKa = 11.84LTQMTAIRR119 pKa = 11.84DD120 pKa = 3.68ALLSDD125 pKa = 4.53FRR127 pKa = 11.84STGDD131 pKa = 3.5LVTVSDD137 pKa = 3.7KK138 pKa = 10.37YY139 pKa = 11.55LRR141 pKa = 11.84MVAILILNNRR151 pKa = 11.84DD152 pKa = 3.7SLPNPYY158 pKa = 10.1AAYY161 pKa = 10.84NLLQYY166 pKa = 10.65ASKK169 pKa = 10.54RR170 pKa = 11.84GLVNPEE176 pKa = 3.56QAA178 pKa = 3.88

Molecular weight: 19.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0VDC9|A0A0A0VDC9_9VIRU Uncharacterized protein OS=Common midwife toad virus OX=540070 GN=orf72R PE=4 SV=1
MM1 pKa = 7.15YY2 pKa = 10.28NVRR5 pKa = 11.84NSGCSVGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVRR44 pKa = 11.84KK45 pKa = 9.68RR46 pKa = 11.84KK47 pKa = 10.2SLVKK51 pKa = 10.23RR52 pKa = 11.84KK53 pKa = 10.2SPVKK57 pKa = 10.09RR58 pKa = 11.84SPVKK62 pKa = 10.36RR63 pKa = 11.84KK64 pKa = 10.25SPVKK68 pKa = 10.18KK69 pKa = 10.03RR70 pKa = 11.84SPVKK74 pKa = 10.22KK75 pKa = 9.83RR76 pKa = 11.84SPVKK80 pKa = 10.22KK81 pKa = 9.83RR82 pKa = 11.84SPVKK86 pKa = 10.22KK87 pKa = 9.83RR88 pKa = 11.84SPVKK92 pKa = 10.22KK93 pKa = 9.83RR94 pKa = 11.84SPVKK98 pKa = 10.22KK99 pKa = 9.83RR100 pKa = 11.84SPVKK104 pKa = 10.22KK105 pKa = 9.83RR106 pKa = 11.84SPVKK110 pKa = 10.22KK111 pKa = 9.83RR112 pKa = 11.84SPVKK116 pKa = 10.22KK117 pKa = 9.83RR118 pKa = 11.84SPVKK122 pKa = 10.22KK123 pKa = 9.83RR124 pKa = 11.84SPVKK128 pKa = 10.22KK129 pKa = 9.83RR130 pKa = 11.84SPVKK134 pKa = 10.22KK135 pKa = 9.83RR136 pKa = 11.84SPVKK140 pKa = 10.22KK141 pKa = 9.83RR142 pKa = 11.84SPVKK146 pKa = 10.22KK147 pKa = 9.83RR148 pKa = 11.84SPVKK152 pKa = 10.22KK153 pKa = 9.83RR154 pKa = 11.84SPVKK158 pKa = 10.1KK159 pKa = 9.95RR160 pKa = 11.84SPAKK164 pKa = 10.08RR165 pKa = 11.84RR166 pKa = 11.84QQVCFMATKK175 pKa = 10.43

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

30340

16

1294

291.7

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.041 ± 0.245

2.09 ± 0.131

5.992 ± 0.149

5.6 ± 0.244

3.279 ± 0.124

7.343 ± 0.217

2.096 ± 0.166

3.191 ± 0.125

5.438 ± 0.268

7.98 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.032 ± 0.136

2.498 ± 0.131

6.556 ± 0.35

2.943 ± 0.277

7.241 ± 0.303

7.291 ± 0.206

5.606 ± 0.2

8.418 ± 0.205

1.417 ± 0.099

2.947 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski