Klebsiella phage Soft

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Yonseivirus; Klebsiella virus Soft

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K9D4|A0A5C1K9D4_9CAUD Uncharacterized protein OS=Klebsiella phage Soft OX=2601626 GN=CPTSoftv3_045 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.35KK3 pKa = 10.17RR4 pKa = 11.84LTVLICNDD12 pKa = 3.19MRR14 pKa = 11.84DD15 pKa = 3.51PSDD18 pKa = 3.47PVVVTVSAASPIDD31 pKa = 3.88VILQSKK37 pKa = 8.75IAHH40 pKa = 6.71AEE42 pKa = 3.94AEE44 pKa = 4.19MSDD47 pKa = 3.8FDD49 pKa = 5.07GDD51 pKa = 3.76PMTAADD57 pKa = 3.67LWEE60 pKa = 4.08VSRR63 pKa = 11.84AYY65 pKa = 9.73IFAAIPGTVDD75 pKa = 3.84FYY77 pKa = 11.84YY78 pKa = 10.84VGEE81 pKa = 4.4PSDD84 pKa = 3.68TVDD87 pKa = 4.22NYY89 pKa = 10.9DD90 pKa = 3.42HH91 pKa = 7.49VMGEE95 pKa = 4.42CLEE98 pKa = 4.14EE99 pKa = 5.09SPLCTMGEE107 pKa = 4.0DD108 pKa = 3.84HH109 pKa = 6.93EE110 pKa = 4.55

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K8C5|A0A5C1K8C5_9CAUD Putative metalochaperone OS=Klebsiella phage Soft OX=2601626 GN=CPTSoftv3_032 PE=4 SV=1
MM1 pKa = 7.39ASKK4 pKa = 10.48RR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.47SCEE13 pKa = 3.7GKK15 pKa = 9.58QRR17 pKa = 11.84YY18 pKa = 8.26PDD20 pKa = 3.55IPSAMVGIRR29 pKa = 11.84HH30 pKa = 5.59IQRR33 pKa = 11.84TYY35 pKa = 10.79GYY37 pKa = 9.01SGPMDD42 pKa = 5.03AYY44 pKa = 10.55HH45 pKa = 7.16CKK47 pKa = 10.54LCGKK51 pKa = 9.45IHH53 pKa = 6.11IGHH56 pKa = 6.8RR57 pKa = 11.84KK58 pKa = 9.68GIGSHH63 pKa = 6.17RR64 pKa = 11.84PMGRR68 pKa = 11.84KK69 pKa = 8.69

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

18499

34

1435

212.6

23.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.395 ± 0.416

1.135 ± 0.129

6.384 ± 0.21

5.941 ± 0.318

3.638 ± 0.169

7.422 ± 0.233

1.622 ± 0.15

5.417 ± 0.151

5.633 ± 0.337

7.487 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.027 ± 0.13

4.346 ± 0.192

4.352 ± 0.254

3.4 ± 0.305

6.346 ± 0.267

5.4 ± 0.256

5.752 ± 0.264

7.033 ± 0.179

1.919 ± 0.162

3.352 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski