Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Caulobacter; Caulobacter vibrioides

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9A5H1|Q9A5H1_CAUVC Uncharacterized protein OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=CC_2476 PE=4 SV=1
MM1 pKa = 7.15SAEE4 pKa = 4.13PFKK7 pKa = 10.98VWQCRR12 pKa = 11.84TCGYY16 pKa = 10.22IYY18 pKa = 10.38DD19 pKa = 4.58EE20 pKa = 4.41EE21 pKa = 4.52QGDD24 pKa = 4.14PGEE27 pKa = 4.38GLAPGTRR34 pKa = 11.84WADD37 pKa = 3.25IPAGWVCPLCGTPKK51 pKa = 10.44SDD53 pKa = 3.9FDD55 pKa = 4.21MIEE58 pKa = 3.7LL59 pKa = 3.99

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q45979|Y952_CAUVC Uncharacterized protein CC_0952 OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=CC_0952 PE=2 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.26GYY18 pKa = 7.96RR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11NGQKK29 pKa = 9.79VVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3720

0

3720

1205719

30

2479

324.1

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.79 ± 0.057

0.745 ± 0.013

5.795 ± 0.03

5.405 ± 0.038

3.547 ± 0.024

8.974 ± 0.041

1.797 ± 0.02

4.373 ± 0.029

3.517 ± 0.032

10.003 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.02

2.372 ± 0.026

5.485 ± 0.034

3.212 ± 0.028

7.318 ± 0.047

5.104 ± 0.035

5.233 ± 0.045

7.548 ± 0.032

1.412 ± 0.021

2.104 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski