Amino acid dipepetide frequency for Sphingomonas sp. MM-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.84AlaAla: 21.84 ± 0.206	1.061AlaCys: 1.061 ± 0.03	8.708AlaAsp: 8.708 ± 0.085	8.378AlaGlu: 8.378 ± 0.106	4.487AlaPhe: 4.487 ± 0.066	12.869AlaGly: 12.869 ± 0.158	2.395AlaHis: 2.395 ± 0.046	7.581AlaIle: 7.581 ± 0.096	3.771AlaLys: 3.771 ± 0.066	14.47AlaLeu: 14.47 ± 0.136	4.091AlaMet: 4.091 ± 0.066	3.066AlaAsn: 3.066 ± 0.055	6.8AlaPro: 6.8 ± 0.085	4.123AlaGln: 4.123 ± 0.057	11.133AlaArg: 11.133 ± 0.131	6.327AlaSer: 6.327 ± 0.074	6.692AlaThr: 6.692 ± 0.077	9.098AlaVal: 9.098 ± 0.101	1.778AlaTrp: 1.778 ± 0.041	2.769AlaTyr: 2.769 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.953CysAla: 0.953 ± 0.026	0.089CysCys: 0.089 ± 0.009	0.444CysAsp: 0.444 ± 0.019	0.346CysGlu: 0.346 ± 0.015	0.263CysPhe: 0.263 ± 0.013	0.82CysGly: 0.82 ± 0.03	0.221CysHis: 0.221 ± 0.015	0.327CysIle: 0.327 ± 0.015	0.134CysLys: 0.134 ± 0.01	0.667CysLeu: 0.667 ± 0.023	0.136CysMet: 0.136 ± 0.011	0.154CysAsn: 0.154 ± 0.011	0.452CysPro: 0.452 ± 0.019	0.184CysGln: 0.184 ± 0.012	0.574CysArg: 0.574 ± 0.024	0.368CysSer: 0.368 ± 0.018	0.339CysThr: 0.339 ± 0.018	0.48CysVal: 0.48 ± 0.021	0.109CysTrp: 0.109 ± 0.008	0.163CysTyr: 0.163 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.285AspAla: 8.285 ± 0.096	0.458AspCys: 0.458 ± 0.017	3.32AspAsp: 3.32 ± 0.056	3.353AspGlu: 3.353 ± 0.063	2.156AspPhe: 2.156 ± 0.038	5.993AspGly: 5.993 ± 0.089	1.423AspHis: 1.423 ± 0.034	3.008AspIle: 3.008 ± 0.046	1.498AspLys: 1.498 ± 0.037	5.778AspLeu: 5.778 ± 0.073	1.381AspMet: 1.381 ± 0.032	1.325AspAsn: 1.325 ± 0.033	4.0AspPro: 4.0 ± 0.068	1.794AspGln: 1.794 ± 0.036	5.506AspArg: 5.506 ± 0.065	2.184AspSer: 2.184 ± 0.047	2.341AspThr: 2.341 ± 0.042	4.139AspVal: 4.139 ± 0.053	1.079AspTrp: 1.079 ± 0.03	1.609AspTyr: 1.609 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	8.326GluAla: 8.326 ± 0.106	0.303GluCys: 0.303 ± 0.016	2.69GluAsp: 2.69 ± 0.046	2.963GluGlu: 2.963 ± 0.058	1.442GluPhe: 1.442 ± 0.035	4.769GluGly: 4.769 ± 0.065	1.049GluHis: 1.049 ± 0.028	2.931GluIle: 2.931 ± 0.052	1.886GluLys: 1.886 ± 0.048	4.796GluLeu: 4.796 ± 0.069	1.452GluMet: 1.452 ± 0.036	1.325GluAsn: 1.325 ± 0.032	2.634GluPro: 2.634 ± 0.052	1.843GluGln: 1.843 ± 0.037	5.036GluArg: 5.036 ± 0.075	2.149GluSer: 2.149 ± 0.042	3.086GluThr: 3.086 ± 0.045	3.44GluVal: 3.44 ± 0.053	0.756GluTrp: 0.756 ± 0.023	1.006GluTyr: 1.006 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.777PheAla: 4.777 ± 0.067	0.276PheCys: 0.276 ± 0.014	2.643PheAsp: 2.643 ± 0.052	1.871PheGlu: 1.871 ± 0.038	1.187PhePhe: 1.187 ± 0.038	3.552PheGly: 3.552 ± 0.059	0.744PheHis: 0.744 ± 0.027	1.387PheIle: 1.387 ± 0.033	0.765PheLys: 0.765 ± 0.028	3.024PheLeu: 3.024 ± 0.058	0.686PheMet: 0.686 ± 0.024	1.002PheAsn: 1.002 ± 0.029	1.484PhePro: 1.484 ± 0.036	0.934PheGln: 0.934 ± 0.027	2.384PheArg: 2.384 ± 0.046	1.879PheSer: 1.879 ± 0.039	1.99PheThr: 1.99 ± 0.042	2.507PheVal: 2.507 ± 0.045	0.529PheTrp: 0.529 ± 0.018	0.844PheTyr: 0.844 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.527GlyAla: 10.527 ± 0.131	0.782GlyCys: 0.782 ± 0.027	5.266GlyAsp: 5.266 ± 0.069	4.99GlyGlu: 4.99 ± 0.071	3.597GlyPhe: 3.597 ± 0.056	8.777GlyGly: 8.777 ± 0.166	1.975GlyHis: 1.975 ± 0.04	4.636GlyIle: 4.636 ± 0.06	3.016GlyLys: 3.016 ± 0.048	9.152GlyLeu: 9.152 ± 0.09	2.378GlyMet: 2.378 ± 0.044	2.197GlyAsn: 2.197 ± 0.052	3.849GlyPro: 3.849 ± 0.053	3.022GlyGln: 3.022 ± 0.051	7.519GlyArg: 7.519 ± 0.085	4.598GlySer: 4.598 ± 0.087	4.889GlyThr: 4.889 ± 0.154	6.235GlyVal: 6.235 ± 0.078	1.719GlyTrp: 1.719 ± 0.038	2.279GlyTyr: 2.279 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.517HisAla: 2.517 ± 0.049	0.211HisCys: 0.211 ± 0.013	1.176HisAsp: 1.176 ± 0.031	0.931HisGlu: 0.931 ± 0.024	0.82HisPhe: 0.82 ± 0.027	2.038HisGly: 2.038 ± 0.042	0.55HisHis: 0.55 ± 0.023	0.979HisIle: 0.979 ± 0.026	0.412HisLys: 0.412 ± 0.018	1.898HisLeu: 1.898 ± 0.036	0.454HisMet: 0.454 ± 0.02	0.427HisAsn: 0.427 ± 0.018	1.312HisPro: 1.312 ± 0.037	0.543HisGln: 0.543 ± 0.022	1.566HisArg: 1.566 ± 0.034	0.841HisSer: 0.841 ± 0.024	0.611HisThr: 0.611 ± 0.024	1.476HisVal: 1.476 ± 0.038	0.336HisTrp: 0.336 ± 0.017	0.576HisTyr: 0.576 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.544IleAla: 8.544 ± 0.096	0.36IleCys: 0.36 ± 0.02	4.028IleAsp: 4.028 ± 0.062	3.462IleGlu: 3.462 ± 0.053	1.576IlePhe: 1.576 ± 0.034	5.446IleGly: 5.446 ± 0.066	0.922IleHis: 0.922 ± 0.026	1.988IleIle: 1.988 ± 0.044	1.039IleLys: 1.039 ± 0.031	4.132IleLeu: 4.132 ± 0.07	0.765IleMet: 0.765 ± 0.027	1.271IleAsn: 1.271 ± 0.041	2.181IlePro: 2.181 ± 0.037	1.16IleGln: 1.16 ± 0.03	3.559IleArg: 3.559 ± 0.052	2.523IleSer: 2.523 ± 0.044	2.416IleThr: 2.416 ± 0.047	4.251IleVal: 4.251 ± 0.063	0.598IleTrp: 0.598 ± 0.023	0.97IleTyr: 0.97 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.123LysAla: 4.123 ± 0.071	0.118LysCys: 0.118 ± 0.01	1.455LysAsp: 1.455 ± 0.044	1.159LysGlu: 1.159 ± 0.035	0.7LysPhe: 0.7 ± 0.026	2.486LysGly: 2.486 ± 0.047	0.454LysHis: 0.454 ± 0.019	1.285LysIle: 1.285 ± 0.034	0.873LysLys: 0.873 ± 0.03	2.902LysLeu: 2.902 ± 0.057	0.617LysMet: 0.617 ± 0.025	0.623LysAsn: 0.623 ± 0.024	1.836LysPro: 1.836 ± 0.042	0.806LysGln: 0.806 ± 0.023	2.067LysArg: 2.067 ± 0.045	1.431LysSer: 1.431 ± 0.035	1.472LysThr: 1.472 ± 0.041	2.036LysVal: 2.036 ± 0.044	0.338LysTrp: 0.338 ± 0.016	0.527LysTyr: 0.527 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	15.196LeuAla: 15.196 ± 0.15	0.729LeuCys: 0.729 ± 0.027	6.237LeuAsp: 6.237 ± 0.07	4.739LeuGlu: 4.739 ± 0.067	3.451LeuPhe: 3.451 ± 0.066	8.483LeuGly: 8.483 ± 0.094	1.769LeuHis: 1.769 ± 0.043	5.025LeuIle: 5.025 ± 0.068	3.005LeuLys: 3.005 ± 0.049	9.364LeuLeu: 9.364 ± 0.116	2.092LeuMet: 2.092 ± 0.044	2.383LeuAsn: 2.383 ± 0.045	5.685LeuPro: 5.685 ± 0.076	2.325LeuGln: 2.325 ± 0.049	7.114LeuArg: 7.114 ± 0.078	5.652LeuSer: 5.652 ± 0.065	5.092LeuThr: 5.092 ± 0.078	7.138LeuVal: 7.138 ± 0.08	1.281LeuTrp: 1.281 ± 0.035	1.91LeuTyr: 1.91 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	3.539MetAla: 3.539 ± 0.061	0.154MetCys: 0.154 ± 0.011	1.067MetAsp: 1.067 ± 0.028	1.078MetGlu: 1.078 ± 0.031	0.67MetPhe: 0.67 ± 0.023	1.819MetGly: 1.819 ± 0.043	0.417MetHis: 0.417 ± 0.016	1.332MetIle: 1.332 ± 0.034	0.857MetLys: 0.857 ± 0.025	2.591MetLeu: 2.591 ± 0.051	0.618MetMet: 0.618 ± 0.026	0.634MetAsn: 0.634 ± 0.023	1.452MetPro: 1.452 ± 0.032	0.647MetGln: 0.647 ± 0.022	1.854MetArg: 1.854 ± 0.038	1.301MetSer: 1.301 ± 0.03	1.604MetThr: 1.604 ± 0.036	1.523MetVal: 1.523 ± 0.035	0.225MetTrp: 0.225 ± 0.013	0.236MetTyr: 0.236 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.12AsnAla: 3.12 ± 0.053	0.198AsnCys: 0.198 ± 0.012	1.288AsnAsp: 1.288 ± 0.038	1.102AsnGlu: 1.102 ± 0.029	0.873AsnPhe: 0.873 ± 0.027	2.455AsnGly: 2.455 ± 0.067	0.43AsnHis: 0.43 ± 0.018	1.296AsnIle: 1.296 ± 0.036	0.562AsnLys: 0.562 ± 0.021	2.429AsnLeu: 2.429 ± 0.047	0.536AsnMet: 0.536 ± 0.018	0.648AsnAsn: 0.648 ± 0.023	1.672AsnPro: 1.672 ± 0.036	0.732AsnGln: 0.732 ± 0.021	1.831AsnArg: 1.831 ± 0.037	1.152AsnSer: 1.152 ± 0.038	0.985AsnThr: 0.985 ± 0.029	1.772AsnVal: 1.772 ± 0.043	0.367AsnTrp: 0.367 ± 0.021	0.657AsnTyr: 0.657 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	8.185ProAla: 8.185 ± 0.102	0.3ProCys: 0.3 ± 0.015	3.881ProAsp: 3.881 ± 0.058	3.538ProGlu: 3.538 ± 0.061	1.922ProPhe: 1.922 ± 0.038	5.097ProGly: 5.097 ± 0.074	1.025ProHis: 1.025 ± 0.032	2.591ProIle: 2.591 ± 0.043	1.316ProLys: 1.316 ± 0.034	4.95ProLeu: 4.95 ± 0.073	1.246ProMet: 1.246 ± 0.033	1.18ProAsn: 1.18 ± 0.029	3.133ProPro: 3.133 ± 0.077	1.584ProGln: 1.584 ± 0.035	3.306ProArg: 3.306 ± 0.059	2.602ProSer: 2.602 ± 0.048	2.466ProThr: 2.466 ± 0.044	4.247ProVal: 4.247 ± 0.064	0.735ProTrp: 0.735 ± 0.024	1.08ProTyr: 1.08 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.388GlnAla: 4.388 ± 0.067	0.186GlnCys: 0.186 ± 0.013	1.358GlnAsp: 1.358 ± 0.028	1.258GlnGlu: 1.258 ± 0.03	1.017GlnPhe: 1.017 ± 0.027	2.447GlnGly: 2.447 ± 0.05	0.533GlnHis: 0.533 ± 0.023	1.645GlnIle: 1.645 ± 0.03	0.82GlnLys: 0.82 ± 0.025	2.757GlnLeu: 2.757 ± 0.053	0.744GlnMet: 0.744 ± 0.029	0.68GlnAsn: 0.68 ± 0.023	1.814GlnPro: 1.814 ± 0.042	1.085GlnGln: 1.085 ± 0.034	2.425GlnArg: 2.425 ± 0.047	1.551GlnSer: 1.551 ± 0.034	1.438GlnThr: 1.438 ± 0.034	2.137GlnVal: 2.137 ± 0.039	0.395GlnTrp: 0.395 ± 0.019	0.575GlnTyr: 0.575 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	10.049ArgAla: 10.049 ± 0.113	0.498ArgCys: 0.498 ± 0.02	4.661ArgAsp: 4.661 ± 0.066	4.113ArgGlu: 4.113 ± 0.065	3.167ArgPhe: 3.167 ± 0.052	5.498ArgGly: 5.498 ± 0.073	1.866ArgHis: 1.866 ± 0.041	4.643ArgIle: 4.643 ± 0.065	2.05ArgLys: 2.05 ± 0.035	8.957ArgLeu: 8.957 ± 0.112	2.022ArgMet: 2.022 ± 0.043	1.867ArgAsn: 1.867 ± 0.043	4.377ArgPro: 4.377 ± 0.066	2.558ArgGln: 2.558 ± 0.043	6.567ArgArg: 6.567 ± 0.088	3.67ArgSer: 3.67 ± 0.058	3.563ArgThr: 3.563 ± 0.051	4.809ArgVal: 4.809 ± 0.069	1.323ArgTrp: 1.323 ± 0.034	1.89ArgTyr: 1.89 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.16SerAla: 6.16 ± 0.07	0.358SerCys: 0.358 ± 0.017	2.803SerAsp: 2.803 ± 0.047	2.218SerGlu: 2.218 ± 0.049	2.094SerPhe: 2.094 ± 0.041	5.24SerGly: 5.24 ± 0.102	0.876SerHis: 0.876 ± 0.026	2.629SerIle: 2.629 ± 0.047	1.157SerLys: 1.157 ± 0.031	4.913SerLeu: 4.913 ± 0.06	1.04SerMet: 1.04 ± 0.029	1.193SerAsn: 1.193 ± 0.029	2.751SerPro: 2.751 ± 0.04	1.421SerGln: 1.421 ± 0.037	3.541SerArg: 3.541 ± 0.053	2.46SerSer: 2.46 ± 0.053	2.458SerThr: 2.458 ± 0.049	3.336SerVal: 3.336 ± 0.051	0.753SerTrp: 0.753 ± 0.02	1.287SerTyr: 1.287 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	6.534ThrAla: 6.534 ± 0.077	0.332ThrCys: 0.332 ± 0.018	2.767ThrAsp: 2.767 ± 0.049	2.212ThrGlu: 2.212 ± 0.04	1.696ThrPhe: 1.696 ± 0.042	5.215ThrGly: 5.215 ± 0.09	0.853ThrHis: 0.853 ± 0.024	2.932ThrIle: 2.932 ± 0.06	1.14ThrLys: 1.14 ± 0.034	5.703ThrLeu: 5.703 ± 0.098	1.064ThrMet: 1.064 ± 0.03	1.189ThrAsn: 1.189 ± 0.038	3.247ThrPro: 3.247 ± 0.048	1.271ThrGln: 1.271 ± 0.032	3.403ThrArg: 3.403 ± 0.056	2.37ThrSer: 2.37 ± 0.055	2.505ThrThr: 2.505 ± 0.053	3.984ThrVal: 3.984 ± 0.06	0.555ThrTrp: 0.555 ± 0.021	1.118ThrTyr: 1.118 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.137ValAla: 10.137 ± 0.103	0.477ValCys: 0.477 ± 0.021	4.54ValAsp: 4.54 ± 0.062	4.447ValGlu: 4.447 ± 0.071	1.957ValPhe: 1.957 ± 0.037	5.359ValGly: 5.359 ± 0.071	1.37ValHis: 1.37 ± 0.029	3.495ValIle: 3.495 ± 0.055	1.989ValLys: 1.989 ± 0.04	6.298ValLeu: 6.298 ± 0.077	1.555ValMet: 1.555 ± 0.031	1.911ValAsn: 1.911 ± 0.052	3.965ValPro: 3.965 ± 0.055	1.963ValGln: 1.963 ± 0.034	5.411ValArg: 5.411 ± 0.066	3.735ValSer: 3.735 ± 0.061	4.33ValThr: 4.33 ± 0.061	5.084ValVal: 5.084 ± 0.063	0.742ValTrp: 0.742 ± 0.026	1.264ValTyr: 1.264 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.487TrpAla: 1.487 ± 0.036	0.119TrpCys: 0.119 ± 0.009	0.748TrpAsp: 0.748 ± 0.025	0.603TrpGlu: 0.603 ± 0.022	0.577TrpPhe: 0.577 ± 0.019	0.94TrpGly: 0.94 ± 0.026	0.333TrpHis: 0.333 ± 0.015	0.685TrpIle: 0.685 ± 0.026	0.487TrpLys: 0.487 ± 0.019	1.798TrpLeu: 1.798 ± 0.041	0.355TrpMet: 0.355 ± 0.014	0.452TrpAsn: 0.452 ± 0.023	0.724TrpPro: 0.724 ± 0.028	0.579TrpGln: 0.579 ± 0.022	1.371TrpArg: 1.371 ± 0.033	0.782TrpSer: 0.782 ± 0.025	0.816TrpThr: 0.816 ± 0.027	0.79TrpVal: 0.79 ± 0.028	0.256TrpTrp: 0.256 ± 0.016	0.28TrpTyr: 0.28 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.75TyrAla: 2.75 ± 0.047	0.214TyrCys: 0.214 ± 0.013	1.552TyrAsp: 1.552 ± 0.039	1.12TyrGlu: 1.12 ± 0.031	0.814TyrPhe: 0.814 ± 0.025	2.1TyrGly: 2.1 ± 0.044	0.466TyrHis: 0.466 ± 0.018	0.798TyrIle: 0.798 ± 0.022	0.53TyrLys: 0.53 ± 0.02	2.094TyrLeu: 2.094 ± 0.043	0.382TyrMet: 0.382 ± 0.016	0.58TyrAsn: 0.58 ± 0.021	1.015TyrPro: 1.015 ± 0.033	0.679TyrGln: 0.679 ± 0.022	1.982TyrArg: 1.982 ± 0.047	1.112TyrSer: 1.112 ± 0.034	0.995TyrThr: 0.995 ± 0.028	1.548TyrVal: 1.548 ± 0.033	0.31TyrTrp: 0.31 ± 0.014	0.571TyrTyr: 0.571 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4252 proteins (1340665 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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