Algoriphagus sanaruensis
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3377 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142ES66|A0A142ES66_9BACT Glyco_trans_2-like domain-containing protein OS=Algoriphagus sanaruensis OX=1727163 GN=AO498_16075 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.49 SSIKK6 pKa = 10.25 SIQYY10 pKa = 10.05 RR11 pKa = 11.84 LIFRR15 pKa = 11.84 SILFCTWLGFSQFGYY30 pKa = 9.83 SQGFNEE36 pKa = 4.43 NEE38 pKa = 4.21 WIFGSCGTGEE48 pKa = 3.84 NSYY51 pKa = 11.46 LSFGKK56 pKa = 10.57 GGSANVQTLPGTVLIGKK73 pKa = 8.77 NNNALAIDD81 pKa = 5.7 PITGQPLFYY90 pKa = 10.35 TNGVLVYY97 pKa = 9.62 DD98 pKa = 4.87 FSGSPIQGSAPGLNGDD114 pKa = 2.81 IDD116 pKa = 4.06 GRR118 pKa = 11.84 QKK120 pKa = 10.8 VATGFLEE127 pKa = 4.09 YY128 pKa = 11.06 NPDD131 pKa = 3.62 GNKK134 pKa = 10.1 LFYY137 pKa = 10.27 IFYY140 pKa = 9.96 LSPSGQLQYY149 pKa = 12.03 ALVDD153 pKa = 3.7 MNAPGQATGNEE164 pKa = 4.1 RR165 pKa = 11.84 PLGQVIEE172 pKa = 4.14 KK173 pKa = 10.34 DD174 pKa = 3.47 QAIGNAEE181 pKa = 3.95 GAVLVVKK188 pKa = 9.38 TKK190 pKa = 10.7 SSPSYY195 pKa = 10.42 LISFSGGNLEE205 pKa = 3.9 SRR207 pKa = 11.84 RR208 pKa = 11.84 FGSTAGSFSLTDD220 pKa = 3.35 SEE222 pKa = 5.63 GISFTPKK229 pKa = 10.55 AIIFDD234 pKa = 3.91 EE235 pKa = 4.66 DD236 pKa = 3.21 AGQLIVIPEE245 pKa = 4.05 NPGEE249 pKa = 4.95 DD250 pKa = 3.0 ILVIPFDD257 pKa = 3.67 TSTGNFGSPQAISEE271 pKa = 4.34 SGGAEE276 pKa = 4.25 DD277 pKa = 4.99 IGGADD282 pKa = 4.69 FSPDD286 pKa = 2.72 GNFIYY291 pKa = 10.35 FSRR294 pKa = 11.84 GNQLFRR300 pKa = 11.84 VPSSDD305 pKa = 4.45 LNATPEE311 pKa = 4.36 VIPTSPTNHH320 pKa = 6.26 QIYY323 pKa = 10.03 DD324 pKa = 3.8 VKK326 pKa = 10.29 TGPDD330 pKa = 3.2 GQLYY334 pKa = 9.59 YY335 pKa = 10.0 IYY337 pKa = 9.73 EE338 pKa = 4.21 EE339 pKa = 4.54 VVGGPQLIGRR349 pKa = 11.84 VTNPNEE355 pKa = 3.66 PDD357 pKa = 3.02 IALVNLEE364 pKa = 4.35 EE365 pKa = 5.21 DD366 pKa = 4.12 PFNGTDD372 pKa = 3.61 FCGTIFPIFAPNTDD386 pKa = 3.38 IDD388 pKa = 4.02 PTVDD392 pKa = 4.1 FTWDD396 pKa = 3.43 PEE398 pKa = 4.37 APCANNPVQLTSEE411 pKa = 4.23 ITPEE415 pKa = 3.68 NYY417 pKa = 9.81 QPVSFNWEE425 pKa = 4.06 FNPPLTDD432 pKa = 3.27 SDD434 pKa = 4.64 GNPLPADD441 pKa = 4.16 FTQEE445 pKa = 3.95 HH446 pKa = 6.46 FLVPQDD452 pKa = 3.46 ATEE455 pKa = 4.45 GSSISVTLTVTFADD469 pKa = 3.85 GTTRR473 pKa = 11.84 NVTKK477 pKa = 10.37 DD478 pKa = 2.94 IPLSEE483 pKa = 4.65 NNLEE487 pKa = 4.47 ANFTPSDD494 pKa = 3.48 TTLCEE499 pKa = 3.94 SCIDD503 pKa = 5.08 LDD505 pKa = 4.68 PLLQAQSAAGQQDD518 pKa = 3.55 GEE520 pKa = 4.38 APGGGGQNYY529 pKa = 10.2 EE530 pKa = 4.32 YY531 pKa = 10.48 FWSNKK536 pKa = 8.37 RR537 pKa = 11.84 DD538 pKa = 3.83 EE539 pKa = 4.35 GWLPKK544 pKa = 10.13 GANEE548 pKa = 3.92 VCRR551 pKa = 11.84 PGLYY555 pKa = 8.81 WVLVRR560 pKa = 11.84 EE561 pKa = 4.76 PGSSCYY567 pKa = 10.42 AYY569 pKa = 10.62 AEE571 pKa = 4.16 IRR573 pKa = 11.84 IKK575 pKa = 9.92 MWDD578 pKa = 3.9 IPDD581 pKa = 3.32 QSNNIWYY588 pKa = 9.5 FGDD591 pKa = 3.94 GAGLDD596 pKa = 4.29 FNPDD600 pKa = 3.6 PDD602 pKa = 5.09 DD603 pKa = 4.29 EE604 pKa = 4.47 NAPTPRR610 pKa = 11.84 PIAQRR615 pKa = 11.84 HH616 pKa = 5.04 PQNIPAGTTTISDD629 pKa = 3.14 QAGQVLFYY637 pKa = 10.81 TDD639 pKa = 5.12 GEE641 pKa = 4.94 SVWDD645 pKa = 4.04 LNGDD649 pKa = 4.35 LMQNGDD655 pKa = 4.38 NIGGDD660 pKa = 3.47 NQATQGVIAVPVPTQPTLFYY680 pKa = 11.14 LFTTQTNAGGTNTVSYY696 pKa = 10.75 SLVDD700 pKa = 3.32 IKK702 pKa = 11.21 SEE704 pKa = 3.94 NQTGVGNVVTKK715 pKa = 11.07 DD716 pKa = 3.16 NFLFSPATEE725 pKa = 3.81 QSAALTAGDD734 pKa = 3.91 TTWVLFHH741 pKa = 7.06 EE742 pKa = 5.11 LGNNTFRR749 pKa = 11.84 SYY751 pKa = 10.99 PVTVNGIGQPVLSSVGSNHH770 pKa = 6.07 GFNSGVGSMKK780 pKa = 10.43 FSPDD784 pKa = 2.97 GTKK787 pKa = 10.28 VAVTITEE794 pKa = 4.48 GGCNKK799 pKa = 10.36 LEE801 pKa = 3.9 IFEE804 pKa = 4.84 FDD806 pKa = 3.04 QSTGEE811 pKa = 3.85 LSEE814 pKa = 4.09 YY815 pKa = 11.06 ARR817 pKa = 11.84 LDD819 pKa = 3.5 LGCNGEE825 pKa = 4.28 VYY827 pKa = 10.68 GLEE830 pKa = 4.09 FSQDD834 pKa = 2.96 SDD836 pKa = 3.63 RR837 pKa = 11.84 VLVSYY842 pKa = 11.03 RR843 pKa = 11.84 NGGPGIEE850 pKa = 4.21 EE851 pKa = 4.5 FIIKK855 pKa = 9.77 PVEE858 pKa = 4.15 NNSTPNAPTCPSCFDD873 pKa = 3.65 NASTRR878 pKa = 11.84 AQRR881 pKa = 11.84 EE882 pKa = 3.94 ACILTTRR889 pKa = 11.84 KK890 pKa = 9.82 ALNGTSSLDD899 pKa = 3.53 LGALQIASDD908 pKa = 3.87 GQIYY912 pKa = 9.41 VAVVGDD918 pKa = 3.68 NRR920 pKa = 11.84 IGQIQVGTGCTAASTFTQDD939 pKa = 4.28 AVLPMPGTSNLGLPSFVQNSGSSIPEE965 pKa = 3.79 PSLTIPTRR973 pKa = 11.84 LCLDD977 pKa = 3.88 PDD979 pKa = 4.05 LGVSGLLEE987 pKa = 4.43 GAGEE991 pKa = 4.18 PDD993 pKa = 2.9 IDD995 pKa = 4.19 SYY997 pKa = 11.62 FWTITHH1003 pKa = 7.02 EE1004 pKa = 4.74 DD1005 pKa = 3.46 GTVIRR1010 pKa = 11.84 NDD1012 pKa = 3.15 FGGPGDD1018 pKa = 3.73 QFQSLDD1024 pKa = 3.89 QIFTLPGKK1032 pKa = 8.81 YY1033 pKa = 7.88 TVEE1036 pKa = 4.11 LRR1038 pKa = 11.84 VDD1040 pKa = 3.26 RR1041 pKa = 11.84 CGDD1044 pKa = 4.0 PIYY1047 pKa = 11.15 YY1048 pKa = 9.18 EE1049 pKa = 3.91 ATGEE1053 pKa = 4.31 LEE1055 pKa = 4.62 VVAPPTLTLAADD1067 pKa = 3.63 ATLCAGNPVTLTAIDD1082 pKa = 4.97 GYY1084 pKa = 11.44 DD1085 pKa = 3.76 PADD1088 pKa = 3.5 GLYY1091 pKa = 10.73 DD1092 pKa = 4.09 FRR1094 pKa = 11.84 WTNAAGQIIGDD1105 pKa = 4.1 EE1106 pKa = 4.26 NSNSITVDD1114 pKa = 2.96 EE1115 pKa = 4.2 EE1116 pKa = 3.98 SIYY1119 pKa = 10.52 RR1120 pKa = 11.84 VEE1122 pKa = 3.9 VSYY1125 pKa = 10.94 RR1126 pKa = 11.84 LPNGLSQEE1134 pKa = 4.14 EE1135 pKa = 3.87 ADD1137 pKa = 5.03 LFEE1140 pKa = 4.91 TCPSTAEE1147 pKa = 4.24 VFVGPAFEE1155 pKa = 5.46 FEE1157 pKa = 4.35 LTQTATEE1164 pKa = 4.32 VCFEE1168 pKa = 4.57 EE1169 pKa = 5.2 ISVTFAPDD1177 pKa = 3.52 TPISGEE1183 pKa = 3.52 WFYY1186 pKa = 11.63 EE1187 pKa = 3.94 RR1188 pKa = 11.84 NGDD1191 pKa = 3.91 GNRR1194 pKa = 11.84 VSLGEE1199 pKa = 3.98 FFEE1202 pKa = 5.15 LEE1204 pKa = 4.0 LWVNTLPGAGLYY1216 pKa = 10.24 DD1217 pKa = 3.45 IYY1219 pKa = 10.92 FVTEE1223 pKa = 3.97 DD1224 pKa = 5.06 PILAGCTVEE1233 pKa = 6.39 KK1234 pKa = 10.61 RR1235 pKa = 11.84 LQLQVNEE1242 pKa = 4.73 LPLFVPAQTSPATDD1256 pKa = 3.52 CATADD1261 pKa = 3.44 GGFEE1265 pKa = 3.58 ITMQADD1271 pKa = 3.25 AAEE1274 pKa = 4.11 VRR1276 pKa = 11.84 IPEE1279 pKa = 4.35 LSLSFTNVSAGDD1291 pKa = 3.97 VIPVANVLPGVYY1303 pKa = 8.75 TVEE1306 pKa = 4.44 AEE1308 pKa = 4.11 SSAGCFYY1315 pKa = 10.82 SGSVTVEE1322 pKa = 3.74 NANPPSGLAFTVATTDD1338 pKa = 3.43 EE1339 pKa = 4.72 TCASTGVNPGIISITFTSGTAQTGTYY1365 pKa = 9.35 TITRR1369 pKa = 11.84 QGDD1372 pKa = 3.69 GSVFTGPLPNQAAFDD1387 pKa = 3.95 VEE1389 pKa = 4.63 VPYY1392 pKa = 10.9 GSYY1395 pKa = 10.39 LVEE1398 pKa = 3.96 VADD1401 pKa = 5.08 ASGCSVPDD1409 pKa = 3.78 PSLYY1413 pKa = 10.77 SVAQKK1418 pKa = 10.75 FEE1420 pKa = 4.54 VVFSVPTSVEE1430 pKa = 3.51 ACEE1433 pKa = 4.58 IFAFTPIGPNTLNYY1447 pKa = 10.6 LVTNSSGTVINPDD1460 pKa = 3.2 ANGEE1464 pKa = 4.09 YY1465 pKa = 10.72 LLTSSGIYY1473 pKa = 7.62 TVRR1476 pKa = 11.84 GEE1478 pKa = 4.42 DD1479 pKa = 3.9 PNGVDD1484 pKa = 4.62 CPRR1487 pKa = 11.84 EE1488 pKa = 3.68 IQMDD1492 pKa = 3.8 VTLSQPIDD1500 pKa = 3.85 FSVSGPIIDD1509 pKa = 4.11 CQAGVRR1515 pKa = 11.84 FEE1517 pKa = 5.06 AVLNNALPQDD1527 pKa = 4.74 VIFLWKK1533 pKa = 10.14 DD1534 pKa = 3.22 DD1535 pKa = 3.96 LGVIVGRR1542 pKa = 11.84 RR1543 pKa = 11.84 QTFTPSRR1550 pKa = 11.84 DD1551 pKa = 3.09 GTYY1554 pKa = 9.97 TLEE1557 pKa = 4.08 VQPASGGLCTTPAQSFTAEE1576 pKa = 4.09 ILDD1579 pKa = 3.87 EE1580 pKa = 4.46 AVTVALEE1587 pKa = 4.02 ADD1589 pKa = 4.79 PYY1591 pKa = 10.9 CISQTSTVIDD1601 pKa = 3.68 ILADD1605 pKa = 3.67 LQNVAEE1611 pKa = 4.51 IEE1613 pKa = 4.08 WFAVSGGTRR1622 pKa = 11.84 TRR1624 pKa = 11.84 LPNFDD1629 pKa = 4.1 NLSSITVTDD1638 pKa = 3.32 EE1639 pKa = 3.81 GTYY1642 pKa = 10.16 EE1643 pKa = 3.95 ALLRR1647 pKa = 11.84 SAQGCEE1653 pKa = 3.77 LGRR1656 pKa = 11.84 ANIVVVKK1663 pKa = 8.74 STIIPPVVPTTPITICAVEE1682 pKa = 5.17 GITTSINPGNYY1693 pKa = 9.87 DD1694 pKa = 3.9 FYY1696 pKa = 11.16 SWRR1699 pKa = 11.84 LNGVEE1704 pKa = 4.27 ISQDD1708 pKa = 3.51 AIFSPTLPGEE1718 pKa = 4.13 YY1719 pKa = 9.91 EE1720 pKa = 4.11 LRR1722 pKa = 11.84 VSDD1725 pKa = 4.64 NLGCEE1730 pKa = 4.0 YY1731 pKa = 10.76 VANFTILEE1739 pKa = 4.16 DD1740 pKa = 3.32 CRR1742 pKa = 11.84 LRR1744 pKa = 11.84 VSYY1747 pKa = 10.72 PNGVVLGDD1755 pKa = 3.66 PNRR1758 pKa = 11.84 NFILYY1763 pKa = 9.88 ANEE1766 pKa = 4.75 FIDD1769 pKa = 3.89 EE1770 pKa = 4.39 VEE1772 pKa = 4.03 VFIFNRR1778 pKa = 11.84 WGEE1781 pKa = 4.09 LIFYY1785 pKa = 9.95 CEE1787 pKa = 4.46 HH1788 pKa = 6.84 EE1789 pKa = 4.33 NLEE1792 pKa = 4.72 PKK1794 pKa = 9.44 TAFCPWDD1801 pKa = 3.46 GQVNGKK1807 pKa = 8.85 FVPNGTYY1814 pKa = 9.86 AVKK1817 pKa = 10.85 VKK1819 pKa = 9.1 FTSRR1823 pKa = 11.84 NQNLTQTEE1831 pKa = 4.52 TKK1833 pKa = 10.3 AITIIQQ1839 pKa = 3.58
Molecular weight: 197.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.732
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.63
Solomon 3.897
Lehninger 3.859
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.808
Sillero 4.05
Patrickios 0.96
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A142EKI2|A0A142EKI2_9BACT Peptidase M23 OS=Algoriphagus sanaruensis OX=1727163 GN=AO498_04410 PE=4 SV=1
MM1 pKa = 6.99 SQEE4 pKa = 3.91 EE5 pKa = 4.29 VLGQLRR11 pKa = 11.84 AVTRR15 pKa = 11.84 EE16 pKa = 3.72 ANFLDD21 pKa = 4.96 ARR23 pKa = 11.84 TQTLADD29 pKa = 3.45 VKK31 pKa = 11.13 FNGLVGRR38 pKa = 11.84 QKK40 pKa = 10.52 FRR42 pKa = 11.84 ISRR45 pKa = 11.84 VIRR48 pKa = 11.84 RR49 pKa = 11.84 GEE51 pKa = 3.96 TFLPLIEE58 pKa = 4.47 GRR60 pKa = 11.84 VEE62 pKa = 3.65 KK63 pKa = 10.01 TPRR66 pKa = 11.84 GSIIFLAYY74 pKa = 10.4 RR75 pKa = 11.84 LFPVALFFLIFWSVVLVSFAIFYY98 pKa = 11.11 GLALEE103 pKa = 4.37 NWQNAMICSLLAVVNYY119 pKa = 6.71 MLCVFFFHH127 pKa = 6.65 RR128 pKa = 11.84 QVKK131 pKa = 9.15 ASRR134 pKa = 11.84 AIFHH138 pKa = 5.81 QLINFHH144 pKa = 6.84 LKK146 pKa = 10.76 GKK148 pKa = 10.0 DD149 pKa = 3.02
Molecular weight: 17.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.516
IPC_protein 10.058
Toseland 10.452
ProMoST 10.116
Dawson 10.584
Bjellqvist 10.277
Wikipedia 10.76
Rodwell 10.847
Grimsley 10.628
Solomon 10.657
Lehninger 10.628
Nozaki 10.452
DTASelect 10.262
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.511
Patrickios 10.599
IPC_peptide 10.672
IPC2_peptide 9.253
IPC2.peptide.svr19 8.567
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3377
0
3377
1184144
29
4663
350.6
39.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.821 ± 0.052
0.656 ± 0.015
5.149 ± 0.03
6.904 ± 0.042
5.378 ± 0.035
7.355 ± 0.062
1.85 ± 0.026
7.106 ± 0.035
6.506 ± 0.045
10.032 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.023
4.725 ± 0.033
4.148 ± 0.024
4.042 ± 0.03
4.075 ± 0.031
6.742 ± 0.031
5.159 ± 0.068
6.179 ± 0.033
1.35 ± 0.017
3.514 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here