Hubei sobemo-like virus 25

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEJ6|A0A1L3KEJ6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 25 OX=1923211 PE=4 SV=1
MM1 pKa = 7.96PPPDD5 pKa = 3.34QRR7 pKa = 11.84SRR9 pKa = 11.84ILRR12 pKa = 11.84RR13 pKa = 11.84LYY15 pKa = 9.86MLYY18 pKa = 10.7SDD20 pKa = 4.54VTWSIPSDD28 pKa = 3.77FLSRR32 pKa = 11.84DD33 pKa = 2.87HH34 pKa = 6.72FRR36 pKa = 11.84RR37 pKa = 11.84VVLNLDD43 pKa = 3.76YY44 pKa = 10.3TSSPGYY50 pKa = 9.66PYY52 pKa = 9.77MRR54 pKa = 11.84SYY56 pKa = 8.44PTNRR60 pKa = 11.84DD61 pKa = 2.97MLKK64 pKa = 10.06PDD66 pKa = 3.67EE67 pKa = 5.7DD68 pKa = 4.47GNPDD72 pKa = 4.61PIRR75 pKa = 11.84EE76 pKa = 3.96EE77 pKa = 4.95LLWQMVQTRR86 pKa = 11.84LQEE89 pKa = 3.86GDD91 pKa = 3.32MDD93 pKa = 5.74PIRR96 pKa = 11.84LFIKK100 pKa = 10.32PEE102 pKa = 3.56PHH104 pKa = 6.85KK105 pKa = 10.78LKK107 pKa = 10.53KK108 pKa = 10.66LEE110 pKa = 3.75GHH112 pKa = 7.08RR113 pKa = 11.84YY114 pKa = 9.05RR115 pKa = 11.84LISSVSVVDD124 pKa = 3.85QVVHH128 pKa = 6.58AMLFGVLNDD137 pKa = 3.91KK138 pKa = 10.68MSDD141 pKa = 2.9NWPYY145 pKa = 10.78IPNKK149 pKa = 10.21SGWSPHH155 pKa = 5.3YY156 pKa = 10.24GGYY159 pKa = 10.43RR160 pKa = 11.84NIPLTGWMATDD171 pKa = 3.28KK172 pKa = 11.47SGWDD176 pKa = 3.27WSAQPWTFEE185 pKa = 3.95MVFTLRR191 pKa = 11.84KK192 pKa = 8.77MLCQNLEE199 pKa = 4.06EE200 pKa = 4.57HH201 pKa = 6.71PLWEE205 pKa = 4.19KK206 pKa = 10.59LAARR210 pKa = 11.84AYY212 pKa = 8.7QQLYY216 pKa = 8.52TDD218 pKa = 3.82PVFINTYY225 pKa = 9.87GHH227 pKa = 7.97LLQQKK232 pKa = 9.74KK233 pKa = 10.31PGVQKK238 pKa = 10.64SGCFNTLADD247 pKa = 3.62NCIQQVIIHH256 pKa = 5.83YY257 pKa = 8.38RR258 pKa = 11.84VCDD261 pKa = 4.14DD262 pKa = 4.74LGLAPGQMMCMGDD275 pKa = 4.36DD276 pKa = 4.44VYY278 pKa = 11.42QQTPPDD284 pKa = 3.52KK285 pKa = 10.79DD286 pKa = 3.27RR287 pKa = 11.84YY288 pKa = 9.69LEE290 pKa = 4.46KK291 pKa = 10.72LGEE294 pKa = 4.04YY295 pKa = 10.24CRR297 pKa = 11.84LKK299 pKa = 10.63EE300 pKa = 3.86AAEE303 pKa = 4.14VPEE306 pKa = 3.84FAGYY310 pKa = 10.5RR311 pKa = 11.84FGHH314 pKa = 6.1RR315 pKa = 11.84RR316 pKa = 11.84IEE318 pKa = 3.67PLYY321 pKa = 9.31KK322 pKa = 10.29GKK324 pKa = 9.58HH325 pKa = 5.06AFVLLHH331 pKa = 6.72LNEE334 pKa = 4.29QLAQDD339 pKa = 3.99TLDD342 pKa = 4.1SYY344 pKa = 12.18SLMYY348 pKa = 10.45HH349 pKa = 6.38EE350 pKa = 5.47SRR352 pKa = 11.84DD353 pKa = 3.51RR354 pKa = 11.84DD355 pKa = 3.65FVRR358 pKa = 11.84ALIRR362 pKa = 11.84IMGYY366 pKa = 6.74EE367 pKa = 3.94TRR369 pKa = 11.84PLEE372 pKa = 3.92WADD375 pKa = 3.98EE376 pKa = 4.25IFHH379 pKa = 6.02GHH381 pKa = 5.86

Molecular weight: 44.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEJ6|A0A1L3KEJ6_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 25 OX=1923211 PE=4 SV=1
MM1 pKa = 7.37NKK3 pKa = 9.29VLLAIVPFFLWGVWDD18 pKa = 3.92VVDD21 pKa = 3.99MVLGGSLNEE30 pKa = 3.99RR31 pKa = 11.84VVKK34 pKa = 10.3AVKK37 pKa = 9.17TRR39 pKa = 11.84LGIQEE44 pKa = 3.95EE45 pKa = 4.63PKK47 pKa = 10.41TGFAAAAEE55 pKa = 4.18TASDD59 pKa = 3.21IFYY62 pKa = 10.49GATNATEE69 pKa = 4.5RR70 pKa = 11.84IFIQMGAGSTMEE82 pKa = 4.96EE83 pKa = 4.06YY84 pKa = 10.8AVWIKK89 pKa = 10.28TMLYY93 pKa = 9.84HH94 pKa = 6.96SLLILVFLACNAAVIVLLIGFVRR117 pKa = 11.84MLYY120 pKa = 10.57NKK122 pKa = 10.18ASDD125 pKa = 4.19WIVQPAVWKK134 pKa = 10.39LRR136 pKa = 11.84GVSYY140 pKa = 10.28EE141 pKa = 4.0AIRR144 pKa = 11.84PGSGFVPAEE153 pKa = 3.65IPKK156 pKa = 10.06YY157 pKa = 9.9QISVCKK163 pKa = 9.79PGLFADD169 pKa = 3.84EE170 pKa = 4.4HH171 pKa = 7.18SGYY174 pKa = 10.76AIRR177 pKa = 11.84VDD179 pKa = 3.67DD180 pKa = 4.94NYY182 pKa = 11.69AVLPRR187 pKa = 11.84HH188 pKa = 5.72VLPDD192 pKa = 3.56TEE194 pKa = 4.45RR195 pKa = 11.84VMLVGATGRR204 pKa = 11.84RR205 pKa = 11.84IALACPCLEE214 pKa = 4.27SSKK217 pKa = 11.03HH218 pKa = 5.68PDD220 pKa = 3.22LVYY223 pKa = 10.2MYY225 pKa = 10.35VGKK228 pKa = 10.5DD229 pKa = 2.94NFTKK233 pKa = 10.75LGATLAPVAEE243 pKa = 4.55SEE245 pKa = 4.5GGHH248 pKa = 5.27ATCVGPLGATAGRR261 pKa = 11.84LTKK264 pKa = 10.21GAQVGTLYY272 pKa = 11.23YY273 pKa = 10.59NGSTVAGMSGAAYY286 pKa = 10.11VIGGRR291 pKa = 11.84VVGIHH296 pKa = 6.87HH297 pKa = 6.62GTLDD301 pKa = 3.37NKK303 pKa = 10.39KK304 pKa = 10.31FNFGTATQLISLEE317 pKa = 3.95LRR319 pKa = 11.84NLVTFEE325 pKa = 4.11SSEE328 pKa = 4.06DD329 pKa = 3.58LGVKK333 pKa = 10.2AGFTMEE339 pKa = 4.47EE340 pKa = 4.38DD341 pKa = 4.04DD342 pKa = 6.04DD343 pKa = 4.83EE344 pKa = 4.48EE345 pKa = 4.45TLGVKK350 pKa = 9.42GRR352 pKa = 11.84KK353 pKa = 8.68KK354 pKa = 10.76AEE356 pKa = 3.78AAKK359 pKa = 10.36KK360 pKa = 9.76RR361 pKa = 11.84SAWNNQDD368 pKa = 2.7VVNYY372 pKa = 7.03VHH374 pKa = 7.36RR375 pKa = 11.84KK376 pKa = 8.33DD377 pKa = 4.44DD378 pKa = 3.56VVGGMFVGTSWADD391 pKa = 3.45LLDD394 pKa = 4.2PRR396 pKa = 11.84DD397 pKa = 3.44GFYY400 pKa = 10.58EE401 pKa = 4.61GEE403 pKa = 4.13STRR406 pKa = 11.84PKK408 pKa = 8.36TQKK411 pKa = 10.18KK412 pKa = 9.41KK413 pKa = 11.05LIVTVPEE420 pKa = 4.13ASVSFTPQNQTGSGQLVMPVRR441 pKa = 11.84CDD443 pKa = 3.53EE444 pKa = 4.41SCSTRR449 pKa = 11.84LEE451 pKa = 4.21SLALEE456 pKa = 4.35VNEE459 pKa = 4.9ALAALEE465 pKa = 4.0KK466 pKa = 10.67RR467 pKa = 11.84LTAVEE472 pKa = 4.18TEE474 pKa = 4.41MVRR477 pKa = 11.84RR478 pKa = 11.84TAPKK482 pKa = 10.2DD483 pKa = 3.2PHH485 pKa = 6.96PCDD488 pKa = 3.34QCSVICTSEE497 pKa = 4.06EE498 pKa = 3.51KK499 pKa = 10.77LLNHH503 pKa = 6.87RR504 pKa = 11.84RR505 pKa = 11.84TSHH508 pKa = 6.0PVSYY512 pKa = 10.8SCTHH516 pKa = 6.69CSFSDD521 pKa = 4.06PSASEE526 pKa = 3.67LAAHH530 pKa = 6.66VKK532 pKa = 8.91TVHH535 pKa = 7.14KK536 pKa = 9.37ATQYY540 pKa = 11.13KK541 pKa = 9.52CDD543 pKa = 4.06CGVEE547 pKa = 4.32CRR549 pKa = 11.84SEE551 pKa = 3.85LRR553 pKa = 11.84LRR555 pKa = 11.84NHH557 pKa = 5.86QEE559 pKa = 3.45SCKK562 pKa = 9.92RR563 pKa = 11.84VSNMPPMEE571 pKa = 4.68GEE573 pKa = 4.04SAIPSDD579 pKa = 3.49TGATSKK585 pKa = 9.29TVKK588 pKa = 10.07QRR590 pKa = 11.84VPFLGRR596 pKa = 11.84TSSSPRR602 pKa = 11.84RR603 pKa = 11.84TTRR606 pKa = 11.84PSLRR610 pKa = 11.84SSTSSGTRR618 pKa = 11.84SPSPALEE625 pKa = 5.22GILSQMTEE633 pKa = 3.99SLKK636 pKa = 11.02SIEE639 pKa = 4.55GLLKK643 pKa = 10.39NQPKK647 pKa = 8.66ATAGPSSEE655 pKa = 4.19AQRR658 pKa = 11.84SS659 pKa = 3.48

Molecular weight: 71.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1040

381

659

520.0

58.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.019 ± 1.816

2.115 ± 0.319

5.385 ± 1.312

6.25 ± 0.435

3.077 ± 0.198

7.115 ± 0.79

2.788 ± 0.522

3.75 ± 0.265

5.385 ± 0.389

9.231 ± 0.747

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.662

3.077 ± 0.043

5.769 ± 0.776

3.558 ± 0.842

6.346 ± 0.591

7.404 ± 1.27

5.865 ± 1.291

7.404 ± 1.424

1.635 ± 0.583

3.75 ± 1.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski