Synechococcus phage S-CAM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KLJ8|A0A1D8KLJ8_9CAUD Structural protein OS=Synechococcus phage S-CAM4 OX=1883367 GN=S330809_089 PE=4 SV=1
MM1 pKa = 7.52SNYY4 pKa = 10.54NITDD8 pKa = 3.47DD9 pKa = 3.71SATVDD14 pKa = 3.26RR15 pKa = 11.84LSQQRR20 pKa = 11.84DD21 pKa = 4.13DD22 pKa = 3.93IYY24 pKa = 10.95DD25 pKa = 3.35WCVEE29 pKa = 3.71RR30 pKa = 11.84FRR32 pKa = 11.84YY33 pKa = 9.73HH34 pKa = 6.08MANDD38 pKa = 4.31NIDD41 pKa = 3.67EE42 pKa = 4.48ALALADD48 pKa = 5.58EE49 pKa = 4.92FFEE52 pKa = 4.55WMDD55 pKa = 3.79PDD57 pKa = 4.02GMEE60 pKa = 4.16KK61 pKa = 10.79EE62 pKa = 3.87EE63 pKa = 3.89TAFYY67 pKa = 11.34NEE69 pKa = 4.69DD70 pKa = 3.91DD71 pKa = 4.58LLNLYY76 pKa = 10.64LSLPEE81 pKa = 4.48NYY83 pKa = 10.3GSS85 pKa = 3.52

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KMF3|A0A1D8KMF3_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM4 OX=1883367 GN=C440309_144 PE=4 SV=1
MM1 pKa = 7.74CGFKK5 pKa = 10.53FIILGHH11 pKa = 6.83LGGSHH16 pKa = 6.6PHH18 pKa = 6.81DD19 pKa = 3.87LVFNDD24 pKa = 3.71KK25 pKa = 11.1SRR27 pKa = 11.84VFYY30 pKa = 9.1ITCEE34 pKa = 3.84SMIQFRR40 pKa = 11.84RR41 pKa = 11.84TEE43 pKa = 4.03RR44 pKa = 11.84KK45 pKa = 8.1TEE47 pKa = 3.86CTRR50 pKa = 11.84AKK52 pKa = 9.1CTIYY56 pKa = 8.71FTPNVYY62 pKa = 9.69IRR64 pKa = 11.84NRR66 pKa = 11.84MIPMIHH72 pKa = 5.73THH74 pKa = 5.53TIRR77 pKa = 11.84RR78 pKa = 11.84VSNTRR83 pKa = 11.84MKK85 pKa = 10.44KK86 pKa = 9.19VLPISGEE93 pKa = 3.52NRR95 pKa = 11.84LAVTRR100 pKa = 11.84EE101 pKa = 4.2DD102 pKa = 4.11IIHH105 pKa = 6.44

Molecular weight: 12.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

238

0

238

60812

35

4057

255.5

28.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.472 ± 0.245

0.83 ± 0.078

6.594 ± 0.119

5.884 ± 0.296

4.338 ± 0.125

7.872 ± 0.357

1.414 ± 0.124

6.527 ± 0.165

5.436 ± 0.349

7.24 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.206

6.393 ± 0.191

3.95 ± 0.119

3.588 ± 0.092

3.836 ± 0.13

7.573 ± 0.277

7.836 ± 0.339

6.782 ± 0.215

1.149 ± 0.083

4.294 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski