Amino acid dipepetide frequency for Rathayibacter phage NCPPB3778

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.117AlaAla: 16.117 ± 1.82
0.22AlaCys: 0.22 ± 0.119
6.886AlaAsp: 6.886 ± 0.657
5.934AlaGlu: 5.934 ± 0.646
2.857AlaPhe: 2.857 ± 0.448
9.304AlaGly: 9.304 ± 1.278
1.465AlaHis: 1.465 ± 0.308
6.593AlaIle: 6.593 ± 0.628
3.736AlaLys: 3.736 ± 0.419
9.744AlaLeu: 9.744 ± 1.154
1.758AlaMet: 1.758 ± 0.393
2.857AlaAsn: 2.857 ± 0.469
4.249AlaPro: 4.249 ± 0.772
3.663AlaGln: 3.663 ± 0.446
7.253AlaArg: 7.253 ± 0.727
9.084AlaSer: 9.084 ± 0.91
6.593AlaThr: 6.593 ± 0.763
7.912AlaVal: 7.912 ± 0.776
1.245AlaTrp: 1.245 ± 0.335
2.637AlaTyr: 2.637 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
0.293CysAla: 0.293 ± 0.139
0.0CysCys: 0.0 ± 0.0
0.293CysAsp: 0.293 ± 0.155
0.293CysGlu: 0.293 ± 0.154
0.0CysPhe: 0.0 ± 0.0
0.293CysGly: 0.293 ± 0.128
0.073CysHis: 0.073 ± 0.065
0.0CysIle: 0.0 ± 0.0
0.22CysLys: 0.22 ± 0.156
0.293CysLeu: 0.293 ± 0.169
0.073CysMet: 0.073 ± 0.073
0.293CysAsn: 0.293 ± 0.123
0.44CysPro: 0.44 ± 0.209
0.147CysGln: 0.147 ± 0.099
0.586CysArg: 0.586 ± 0.221
0.22CysSer: 0.22 ± 0.099
0.147CysThr: 0.147 ± 0.095
0.366CysVal: 0.366 ± 0.163
0.147CysTrp: 0.147 ± 0.103
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.886AspAla: 6.886 ± 0.682
0.147AspCys: 0.147 ± 0.105
4.542AspAsp: 4.542 ± 0.62
4.029AspGlu: 4.029 ± 0.622
2.418AspPhe: 2.418 ± 0.372
5.861AspGly: 5.861 ± 0.796
1.026AspHis: 1.026 ± 0.319
3.516AspIle: 3.516 ± 0.52
2.418AspLys: 2.418 ± 0.432
5.348AspLeu: 5.348 ± 0.526
1.978AspMet: 1.978 ± 0.408
1.832AspAsn: 1.832 ± 0.295
3.81AspPro: 3.81 ± 0.676
1.832AspGln: 1.832 ± 0.368
4.103AspArg: 4.103 ± 0.572
4.908AspSer: 4.908 ± 0.613
3.59AspThr: 3.59 ± 0.47
4.103AspVal: 4.103 ± 0.614
1.099AspTrp: 1.099 ± 0.27
1.832AspTyr: 1.832 ± 0.323
0.0AspXaa: 0.0 ± 0.0
Glu
6.886GluAla: 6.886 ± 0.715
0.22GluCys: 0.22 ± 0.117
3.883GluAsp: 3.883 ± 0.626
3.37GluGlu: 3.37 ± 0.631
1.905GluPhe: 1.905 ± 0.4
4.029GluGly: 4.029 ± 0.569
0.806GluHis: 0.806 ± 0.27
3.077GluIle: 3.077 ± 0.424
2.418GluLys: 2.418 ± 0.504
4.762GluLeu: 4.762 ± 0.635
1.612GluMet: 1.612 ± 0.342
1.612GluAsn: 1.612 ± 0.356
1.832GluPro: 1.832 ± 0.462
1.245GluGln: 1.245 ± 0.342
2.857GluArg: 2.857 ± 0.336
4.396GluSer: 4.396 ± 0.626
2.784GluThr: 2.784 ± 0.484
5.275GluVal: 5.275 ± 0.593
1.319GluTrp: 1.319 ± 0.303
1.612GluTyr: 1.612 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
2.784PheAla: 2.784 ± 0.567
0.147PheCys: 0.147 ± 0.099
2.344PheAsp: 2.344 ± 0.434
1.758PheGlu: 1.758 ± 0.343
1.612PhePhe: 1.612 ± 0.258
2.637PheGly: 2.637 ± 0.494
0.073PheHis: 0.073 ± 0.073
1.612PheIle: 1.612 ± 0.409
0.806PheLys: 0.806 ± 0.231
1.758PheLeu: 1.758 ± 0.31
0.659PheMet: 0.659 ± 0.213
1.099PheAsn: 1.099 ± 0.253
1.612PhePro: 1.612 ± 0.409
1.099PheGln: 1.099 ± 0.239
1.172PheArg: 1.172 ± 0.291
3.077PheSer: 3.077 ± 0.335
1.978PheThr: 1.978 ± 0.31
1.978PheVal: 1.978 ± 0.416
0.659PheTrp: 0.659 ± 0.219
1.245PheTyr: 1.245 ± 0.29
0.0PheXaa: 0.0 ± 0.0
Gly
7.985GlyAla: 7.985 ± 0.858
0.366GlyCys: 0.366 ± 0.183
5.348GlyAsp: 5.348 ± 0.765
5.348GlyGlu: 5.348 ± 0.632
2.271GlyPhe: 2.271 ± 0.464
7.326GlyGly: 7.326 ± 1.156
1.392GlyHis: 1.392 ± 0.283
6.227GlyIle: 6.227 ± 1.227
2.564GlyLys: 2.564 ± 0.365
6.667GlyLeu: 6.667 ± 1.267
1.465GlyMet: 1.465 ± 0.374
2.491GlyAsn: 2.491 ± 0.673
3.736GlyPro: 3.736 ± 0.734
2.637GlyGln: 2.637 ± 0.436
5.861GlyArg: 5.861 ± 0.955
6.813GlySer: 6.813 ± 1.062
5.201GlyThr: 5.201 ± 0.582
6.74GlyVal: 6.74 ± 0.732
1.758GlyTrp: 1.758 ± 0.443
3.223GlyTyr: 3.223 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
1.538HisAla: 1.538 ± 0.457
0.147HisCys: 0.147 ± 0.103
1.099HisAsp: 1.099 ± 0.274
1.319HisGlu: 1.319 ± 0.277
0.293HisPhe: 0.293 ± 0.13
1.392HisGly: 1.392 ± 0.386
0.513HisHis: 0.513 ± 0.191
1.245HisIle: 1.245 ± 0.308
0.586HisLys: 0.586 ± 0.187
1.319HisLeu: 1.319 ± 0.289
0.44HisMet: 0.44 ± 0.201
0.366HisAsn: 0.366 ± 0.156
0.952HisPro: 0.952 ± 0.302
0.659HisGln: 0.659 ± 0.235
1.538HisArg: 1.538 ± 0.36
0.952HisSer: 0.952 ± 0.3
0.806HisThr: 0.806 ± 0.253
1.465HisVal: 1.465 ± 0.349
0.073HisTrp: 0.073 ± 0.065
0.733HisTyr: 0.733 ± 0.253
0.0HisXaa: 0.0 ± 0.0
Ile
5.934IleAla: 5.934 ± 0.673
0.44IleCys: 0.44 ± 0.19
4.469IleAsp: 4.469 ± 0.521
3.883IleGlu: 3.883 ± 0.475
1.832IlePhe: 1.832 ± 0.401
4.615IleGly: 4.615 ± 0.467
1.319IleHis: 1.319 ± 0.291
3.077IleIle: 3.077 ± 0.586
2.198IleLys: 2.198 ± 0.346
4.835IleLeu: 4.835 ± 0.492
1.099IleMet: 1.099 ± 0.28
2.564IleAsn: 2.564 ± 0.396
2.344IlePro: 2.344 ± 0.387
1.978IleGln: 1.978 ± 0.427
2.418IleArg: 2.418 ± 0.36
4.615IleSer: 4.615 ± 0.652
4.762IleThr: 4.762 ± 0.727
4.249IleVal: 4.249 ± 0.687
1.099IleTrp: 1.099 ± 0.265
1.612IleTyr: 1.612 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
4.982LysAla: 4.982 ± 0.645
0.147LysCys: 0.147 ± 0.1
1.465LysAsp: 1.465 ± 0.323
2.271LysGlu: 2.271 ± 0.455
1.099LysPhe: 1.099 ± 0.246
2.784LysGly: 2.784 ± 0.513
0.44LysHis: 0.44 ± 0.152
2.491LysIle: 2.491 ± 0.512
1.978LysLys: 1.978 ± 0.489
2.93LysLeu: 2.93 ± 0.481
1.538LysMet: 1.538 ± 0.277
1.245LysAsn: 1.245 ± 0.387
1.392LysPro: 1.392 ± 0.383
0.733LysGln: 0.733 ± 0.224
2.418LysArg: 2.418 ± 0.481
2.784LysSer: 2.784 ± 0.367
2.198LysThr: 2.198 ± 0.357
3.15LysVal: 3.15 ± 0.484
0.44LysTrp: 0.44 ± 0.156
1.832LysTyr: 1.832 ± 0.305
0.0LysXaa: 0.0 ± 0.0
Leu
8.791LeuAla: 8.791 ± 0.825
0.147LeuCys: 0.147 ± 0.09
6.886LeuAsp: 6.886 ± 0.768
4.469LeuGlu: 4.469 ± 0.609
2.344LeuPhe: 2.344 ± 0.376
6.447LeuGly: 6.447 ± 0.772
1.978LeuHis: 1.978 ± 0.366
4.982LeuIle: 4.982 ± 0.807
3.663LeuLys: 3.663 ± 0.559
6.447LeuLeu: 6.447 ± 0.797
1.392LeuMet: 1.392 ± 0.28
1.905LeuAsn: 1.905 ± 0.604
5.348LeuPro: 5.348 ± 0.926
1.832LeuGln: 1.832 ± 0.343
4.029LeuArg: 4.029 ± 0.527
7.839LeuSer: 7.839 ± 0.875
5.275LeuThr: 5.275 ± 0.666
6.593LeuVal: 6.593 ± 1.096
1.026LeuTrp: 1.026 ± 0.256
1.978LeuTyr: 1.978 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
2.344MetAla: 2.344 ± 0.349
0.073MetCys: 0.073 ± 0.08
0.879MetAsp: 0.879 ± 0.202
1.099MetGlu: 1.099 ± 0.28
0.44MetPhe: 0.44 ± 0.166
1.685MetGly: 1.685 ± 0.377
0.293MetHis: 0.293 ± 0.154
1.245MetIle: 1.245 ± 0.289
0.806MetLys: 0.806 ± 0.219
1.392MetLeu: 1.392 ± 0.359
0.513MetMet: 0.513 ± 0.181
0.513MetAsn: 0.513 ± 0.204
1.758MetPro: 1.758 ± 0.333
0.366MetGln: 0.366 ± 0.153
1.319MetArg: 1.319 ± 0.269
2.344MetSer: 2.344 ± 0.347
2.344MetThr: 2.344 ± 0.392
1.099MetVal: 1.099 ± 0.23
0.293MetTrp: 0.293 ± 0.14
0.293MetTyr: 0.293 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
4.249AsnAla: 4.249 ± 0.509
0.0AsnCys: 0.0 ± 0.0
1.319AsnAsp: 1.319 ± 0.377
0.879AsnGlu: 0.879 ± 0.225
1.245AsnPhe: 1.245 ± 0.262
3.883AsnGly: 3.883 ± 1.163
0.293AsnHis: 0.293 ± 0.134
1.612AsnIle: 1.612 ± 0.364
1.099AsnLys: 1.099 ± 0.282
3.223AsnLeu: 3.223 ± 0.553
0.513AsnMet: 0.513 ± 0.212
0.733AsnAsn: 0.733 ± 0.226
1.612AsnPro: 1.612 ± 0.342
0.659AsnGln: 0.659 ± 0.231
1.392AsnArg: 1.392 ± 0.459
1.758AsnSer: 1.758 ± 0.394
1.465AsnThr: 1.465 ± 0.443
1.905AsnVal: 1.905 ± 0.43
0.147AsnTrp: 0.147 ± 0.089
0.513AsnTyr: 0.513 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
4.615ProAla: 4.615 ± 0.593
0.366ProCys: 0.366 ± 0.211
3.516ProAsp: 3.516 ± 0.606
3.663ProGlu: 3.663 ± 0.615
1.392ProPhe: 1.392 ± 0.334
3.81ProGly: 3.81 ± 0.65
0.586ProHis: 0.586 ± 0.225
3.077ProIle: 3.077 ± 0.539
2.125ProLys: 2.125 ± 0.386
3.883ProLeu: 3.883 ± 0.462
0.879ProMet: 0.879 ± 0.248
1.099ProAsn: 1.099 ± 0.282
1.905ProPro: 1.905 ± 0.417
1.465ProGln: 1.465 ± 0.327
2.198ProArg: 2.198 ± 0.354
3.883ProSer: 3.883 ± 0.538
3.004ProThr: 3.004 ± 0.564
2.857ProVal: 2.857 ± 0.39
1.026ProTrp: 1.026 ± 0.243
1.245ProTyr: 1.245 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
2.637GlnAla: 2.637 ± 0.589
0.366GlnCys: 0.366 ± 0.156
1.026GlnAsp: 1.026 ± 0.234
1.832GlnGlu: 1.832 ± 0.351
0.879GlnPhe: 0.879 ± 0.218
1.905GlnGly: 1.905 ± 0.362
1.099GlnHis: 1.099 ± 0.271
3.004GlnIle: 3.004 ± 0.474
1.392GlnLys: 1.392 ± 0.237
3.077GlnLeu: 3.077 ± 0.49
0.366GlnMet: 0.366 ± 0.186
0.733GlnAsn: 0.733 ± 0.201
0.659GlnPro: 0.659 ± 0.192
1.172GlnGln: 1.172 ± 0.288
1.392GlnArg: 1.392 ± 0.348
1.905GlnSer: 1.905 ± 0.338
1.685GlnThr: 1.685 ± 0.283
2.125GlnVal: 2.125 ± 0.44
0.879GlnTrp: 0.879 ± 0.265
1.099GlnTyr: 1.099 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
4.908ArgAla: 4.908 ± 0.691
0.293ArgCys: 0.293 ± 0.139
4.249ArgAsp: 4.249 ± 0.552
2.93ArgGlu: 2.93 ± 0.455
1.758ArgPhe: 1.758 ± 0.279
3.736ArgGly: 3.736 ± 0.515
1.099ArgHis: 1.099 ± 0.26
3.297ArgIle: 3.297 ± 0.402
2.491ArgLys: 2.491 ± 0.363
6.3ArgLeu: 6.3 ± 0.747
1.978ArgMet: 1.978 ± 0.332
1.319ArgAsn: 1.319 ± 0.275
2.857ArgPro: 2.857 ± 0.452
2.125ArgGln: 2.125 ± 0.37
3.736ArgArg: 3.736 ± 0.527
3.59ArgSer: 3.59 ± 0.474
5.055ArgThr: 5.055 ± 0.722
5.128ArgVal: 5.128 ± 0.525
0.586ArgTrp: 0.586 ± 0.218
1.905ArgTyr: 1.905 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
8.132SerAla: 8.132 ± 1.265
0.44SerCys: 0.44 ± 0.152
4.176SerAsp: 4.176 ± 0.661
4.396SerGlu: 4.396 ± 0.534
2.418SerPhe: 2.418 ± 0.322
8.571SerGly: 8.571 ± 0.888
2.198SerHis: 2.198 ± 0.464
4.396SerIle: 4.396 ± 0.518
2.711SerLys: 2.711 ± 0.427
7.326SerLeu: 7.326 ± 0.739
1.685SerMet: 1.685 ± 0.361
2.051SerAsn: 2.051 ± 0.382
4.176SerPro: 4.176 ± 0.663
1.978SerGln: 1.978 ± 0.385
3.736SerArg: 3.736 ± 0.509
5.714SerSer: 5.714 ± 0.675
3.81SerThr: 3.81 ± 0.599
6.227SerVal: 6.227 ± 0.52
1.685SerTrp: 1.685 ± 0.357
2.784SerTyr: 2.784 ± 0.452
0.0SerXaa: 0.0 ± 0.0
Thr
8.205ThrAla: 8.205 ± 0.886
0.147ThrCys: 0.147 ± 0.103
4.176ThrAsp: 4.176 ± 0.608
2.125ThrGlu: 2.125 ± 0.387
2.564ThrPhe: 2.564 ± 0.461
7.179ThrGly: 7.179 ± 1.091
1.026ThrHis: 1.026 ± 0.286
3.59ThrIle: 3.59 ± 0.536
2.125ThrLys: 2.125 ± 0.412
5.348ThrLeu: 5.348 ± 0.686
1.026ThrMet: 1.026 ± 0.263
1.685ThrAsn: 1.685 ± 0.333
3.223ThrPro: 3.223 ± 0.482
2.198ThrGln: 2.198 ± 0.362
4.322ThrArg: 4.322 ± 0.56
4.396ThrSer: 4.396 ± 0.491
4.762ThrThr: 4.762 ± 0.745
4.689ThrVal: 4.689 ± 0.731
1.392ThrTrp: 1.392 ± 0.271
1.832ThrTyr: 1.832 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
8.718ValAla: 8.718 ± 0.9
0.366ValCys: 0.366 ± 0.169
5.421ValAsp: 5.421 ± 0.555
4.396ValGlu: 4.396 ± 0.499
1.685ValPhe: 1.685 ± 0.324
5.495ValGly: 5.495 ± 0.639
1.026ValHis: 1.026 ± 0.232
3.883ValIle: 3.883 ± 0.489
3.443ValLys: 3.443 ± 0.458
5.055ValLeu: 5.055 ± 0.492
1.026ValMet: 1.026 ± 0.291
2.564ValAsn: 2.564 ± 0.512
2.857ValPro: 2.857 ± 0.526
1.758ValGln: 1.758 ± 0.327
4.982ValArg: 4.982 ± 0.595
6.154ValSer: 6.154 ± 0.679
6.74ValThr: 6.74 ± 1.024
7.033ValVal: 7.033 ± 0.83
1.172ValTrp: 1.172 ± 0.267
1.758ValTyr: 1.758 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
1.172TrpAla: 1.172 ± 0.317
0.073TrpCys: 0.073 ± 0.072
0.733TrpAsp: 0.733 ± 0.268
0.806TrpGlu: 0.806 ± 0.206
0.293TrpPhe: 0.293 ± 0.146
1.538TrpGly: 1.538 ± 0.392
0.147TrpHis: 0.147 ± 0.098
1.026TrpIle: 1.026 ± 0.31
0.44TrpLys: 0.44 ± 0.183
1.538TrpLeu: 1.538 ± 0.342
0.586TrpMet: 0.586 ± 0.163
0.586TrpAsn: 0.586 ± 0.189
0.586TrpPro: 0.586 ± 0.172
0.586TrpGln: 0.586 ± 0.225
1.685TrpArg: 1.685 ± 0.363
1.319TrpSer: 1.319 ± 0.322
1.978TrpThr: 1.978 ± 0.599
1.099TrpVal: 1.099 ± 0.242
0.073TrpTrp: 0.073 ± 0.076
0.22TrpTyr: 0.22 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.489
0.073TyrCys: 0.073 ± 0.074
2.564TyrAsp: 2.564 ± 0.426
1.099TyrGlu: 1.099 ± 0.33
0.806TyrPhe: 0.806 ± 0.281
3.004TyrGly: 3.004 ± 0.497
0.586TyrHis: 0.586 ± 0.216
1.392TyrIle: 1.392 ± 0.317
1.099TyrLys: 1.099 ± 0.28
2.125TyrLeu: 2.125 ± 0.437
0.44TyrMet: 0.44 ± 0.161
0.952TyrAsn: 0.952 ± 0.281
1.245TyrPro: 1.245 ± 0.323
1.099TyrGln: 1.099 ± 0.276
2.344TyrArg: 2.344 ± 0.343
2.857TyrSer: 2.857 ± 0.545
1.832TyrThr: 1.832 ± 0.371
1.538TyrVal: 1.538 ± 0.307
0.366TyrTrp: 0.366 ± 0.181
0.806TyrTyr: 0.806 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (13651 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski