Jungle carpet python virus
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8MQI2|A0A2K8MQI2_9MONO N OS=Jungle carpet python virus OX=2016401 PE=4 SV=1
MM1 pKa = 7.81 EE2 pKa = 5.03 SRR4 pKa = 11.84 STEE7 pKa = 3.84 PPQEE11 pKa = 3.63 VDD13 pKa = 3.18 PLYY16 pKa = 11.02 KK17 pKa = 10.29 RR18 pKa = 11.84 IPALEE23 pKa = 4.92 GIPEE27 pKa = 4.17 TLRR30 pKa = 11.84 TQGQQLADD38 pKa = 3.71 SEE40 pKa = 5.11 APTEE44 pKa = 4.15 MEE46 pKa = 4.06 NEE48 pKa = 4.18 SLEE51 pKa = 4.19 VLHH54 pKa = 6.9 EE55 pKa = 3.97 KK56 pKa = 10.37 AKK58 pKa = 9.92 KK59 pKa = 9.33 IKK61 pKa = 10.04 RR62 pKa = 11.84 KK63 pKa = 10.08 RR64 pKa = 11.84 EE65 pKa = 4.05 TTDD68 pKa = 3.09 PPSDD72 pKa = 3.7 YY73 pKa = 11.1 TSDD76 pKa = 4.65 DD77 pKa = 4.63 DD78 pKa = 6.31 GDD80 pKa = 4.33 LPDD83 pKa = 4.7 PLVGKK88 pKa = 10.17 VDD90 pKa = 4.54 SIISKK95 pKa = 9.92 IDD97 pKa = 3.42 SLALLVDD104 pKa = 3.35 KK105 pKa = 10.94 RR106 pKa = 11.84 FVLMEE111 pKa = 4.04 EE112 pKa = 5.12 KK113 pKa = 9.6 IDD115 pKa = 4.05 KK116 pKa = 9.73 LQSDD120 pKa = 3.89 IAYY123 pKa = 9.34 LGSRR127 pKa = 11.84 AAEE130 pKa = 3.9 GRR132 pKa = 11.84 PIQTPVQPFGPPTILPTAPSIYY154 pKa = 9.47 PDD156 pKa = 4.3 LPSSSWDD163 pKa = 3.55 DD164 pKa = 3.58 LGMM167 pKa = 4.7
Molecular weight: 18.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.597
IPC2_protein 4.431
IPC_protein 4.368
Toseland 4.19
ProMoST 4.482
Dawson 4.329
Bjellqvist 4.469
Wikipedia 4.215
Rodwell 4.19
Grimsley 4.088
Solomon 4.317
Lehninger 4.279
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.482
Patrickios 4.177
IPC_peptide 4.317
IPC2_peptide 4.469
IPC2.peptide.svr19 4.422
Protein with the highest isoelectric point:
>tr|A0A2K8MNI2|A0A2K8MNI2_9MONO P OS=Jungle carpet python virus OX=2016401 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 3.29 VTEE5 pKa = 5.11 EE6 pKa = 3.74 IRR8 pKa = 11.84 VIAEE12 pKa = 4.36 LIRR15 pKa = 11.84 RR16 pKa = 11.84 ARR18 pKa = 11.84 LGDD21 pKa = 3.49 GVEE24 pKa = 4.49 VNRR27 pKa = 11.84 TTPRR31 pKa = 11.84 GRR33 pKa = 11.84 PAVQADD39 pKa = 3.09 SSTRR43 pKa = 11.84 RR44 pKa = 11.84 NTRR47 pKa = 11.84 DD48 pKa = 3.46 PEE50 pKa = 4.08 DD51 pKa = 3.48 TRR53 pKa = 11.84 ATTRR57 pKa = 11.84 RR58 pKa = 11.84 LRR60 pKa = 11.84 SSNRR64 pKa = 11.84 DD65 pKa = 2.98 GEE67 pKa = 4.38 RR68 pKa = 11.84 VTRR71 pKa = 11.84 GIARR75 pKa = 11.84 EE76 pKa = 3.92 SEE78 pKa = 4.24 EE79 pKa = 4.23 NKK81 pKa = 10.74 EE82 pKa = 3.9 EE83 pKa = 4.13 TRR85 pKa = 11.84 DD86 pKa = 3.76 NRR88 pKa = 11.84 STEE91 pKa = 3.72
Molecular weight: 10.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 8.799
IPC_protein 9.926
Toseland 10.438
ProMoST 10.452
Dawson 10.438
Bjellqvist 10.262
Wikipedia 10.76
Rodwell 10.204
Grimsley 10.467
Solomon 10.804
Lehninger 10.745
Nozaki 10.409
DTASelect 10.262
Thurlkill 10.409
EMBOSS 10.877
Sillero 10.409
Patrickios 10.204
IPC_peptide 10.804
IPC2_peptide 9.268
IPC2.peptide.svr19 8.705
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3007
91
1727
501.2
56.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.321 ± 0.734
2.361 ± 0.646
4.988 ± 0.868
4.955 ± 0.985
3.658 ± 0.573
5.454 ± 0.163
2.96 ± 0.573
6.718 ± 0.36
5.155 ± 0.39
10.941 ± 1.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.995 ± 0.308
3.558 ± 0.598
5.886 ± 0.974
3.691 ± 0.186
4.922 ± 1.127
9.112 ± 0.349
7.283 ± 0.827
6.518 ± 0.589
1.463 ± 0.222
3.06 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here