Candidatus Similichlamydia laticola
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 750 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369KIX5|A0A369KIX5_9BACT GTPase Obg OS=Candidatus Similichlamydia laticola OX=2170265 GN=obg PE=3 SV=1
MM1 pKa = 7.97 DD2 pKa = 5.98 NIFLILNTSLYY13 pKa = 11.12 SSVSWNFLLPPCGLTDD29 pKa = 3.81 FFRR32 pKa = 11.84 DD33 pKa = 3.01 SLGYY37 pKa = 10.66 VNEE40 pKa = 4.16
Molecular weight: 4.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 3.923
IPC_protein 3.592
Toseland 3.439
ProMoST 3.757
Dawson 3.656
Bjellqvist 4.075
Wikipedia 3.706
Rodwell 3.478
Grimsley 3.389
Solomon 3.541
Lehninger 3.503
Nozaki 3.859
DTASelect 3.986
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.541
IPC2_peptide 3.694
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A369KAY7|A0A369KAY7_9BACT Uncharacterized protein OS=Candidatus Similichlamydia laticola OX=2170265 GN=HAT2_00151 PE=4 SV=1
MM1 pKa = 7.49 VKK3 pKa = 9.13 RR4 pKa = 11.84 TYY6 pKa = 10.29 QPSKK10 pKa = 10.04 IKK12 pKa = 10.24 RR13 pKa = 11.84 QRR15 pKa = 11.84 TFGFRR20 pKa = 11.84 KK21 pKa = 9.95 RR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 RR30 pKa = 11.84 ILARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AAGRR40 pKa = 11.84 VRR42 pKa = 11.84 LSAA45 pKa = 4.12
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
750
0
750
232731
37
1812
310.3
35.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.331 ± 0.073
2.0 ± 0.037
4.245 ± 0.052
7.003 ± 0.089
5.574 ± 0.083
6.232 ± 0.08
2.415 ± 0.045
5.667 ± 0.062
5.435 ± 0.083
12.553 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.038 ± 0.033
2.83 ± 0.053
4.436 ± 0.056
4.218 ± 0.063
5.972 ± 0.073
8.416 ± 0.083
4.654 ± 0.112
6.242 ± 0.079
1.369 ± 0.035
2.369 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here