Mesobacillus zeae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Mesobacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A398BA51|A0A398BA51_9BACI N-acetyl-gamma-glutamyl-phosphate reductase OS=Mesobacillus zeae OX=1917180 GN=argC PE=3 SV=1
MM1 pKa = 7.52SGLAVIQDD9 pKa = 3.2IDD11 pKa = 3.82QLLEE15 pKa = 4.29YY16 pKa = 9.88INQPARR22 pKa = 11.84DD23 pKa = 4.09FHH25 pKa = 6.39QLWSDD30 pKa = 3.16IDD32 pKa = 3.6QLLPEE37 pKa = 5.03INQHH41 pKa = 4.84SQYY44 pKa = 11.02INQTLANPGIFPTDD58 pKa = 3.17NEE60 pKa = 4.22

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A398BCW6|A0A398BCW6_9BACI DUF2515 domain-containing protein OS=Mesobacillus zeae OX=1917180 GN=D1970_07985 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4175

0

4175

1151873

21

1893

275.9

30.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.496 ± 0.047

0.779 ± 0.014

5.022 ± 0.032

7.56 ± 0.047

4.593 ± 0.037

7.349 ± 0.041

2.002 ± 0.019

7.462 ± 0.037

7.038 ± 0.039

9.759 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.02

4.123 ± 0.027

3.661 ± 0.023

3.338 ± 0.025

4.352 ± 0.027

6.179 ± 0.027

5.123 ± 0.022

6.873 ± 0.031

0.994 ± 0.013

3.41 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski