Gorilla smacovirus
Average proteome isoelectric point is 7.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5GST2|A0A0B5GST2_9VIRU Rep protein OS=Gorilla smacovirus OX=1603068 GN=rep PE=4 SV=1
MM1 pKa = 6.39 MAFRR5 pKa = 11.84 KK6 pKa = 9.5 RR7 pKa = 11.84 HH8 pKa = 4.43 GSKK11 pKa = 10.24 VLHH14 pKa = 6.69 QKK16 pKa = 8.52 FQWFFDD22 pKa = 3.94 LQTKK26 pKa = 9.58 ADD28 pKa = 3.74 EE29 pKa = 4.13 MQIMEE34 pKa = 4.47 VQAGGYY40 pKa = 9.04 GVYY43 pKa = 10.16 KK44 pKa = 10.34 RR45 pKa = 11.84 LYY47 pKa = 9.09 PFFSAFKK54 pKa = 8.38 YY55 pKa = 10.37 YY56 pKa = 10.95 KK57 pKa = 10.56 LGGVKK62 pKa = 9.37 MKK64 pKa = 9.33 MIPASTLPVDD74 pKa = 3.87 PTGLSYY80 pKa = 10.68 EE81 pKa = 4.23 AGEE84 pKa = 4.26 NTVDD88 pKa = 4.85 PRR90 pKa = 11.84 DD91 pKa = 3.48 QLTPGLTRR99 pKa = 11.84 ITNGEE104 pKa = 4.09 DD105 pKa = 3.47 VYY107 pKa = 11.64 TDD109 pKa = 3.53 LTGLTGDD116 pKa = 3.71 QQRR119 pKa = 11.84 EE120 pKa = 4.43 LYY122 pKa = 9.6 EE123 pKa = 4.92 SMMIDD128 pKa = 3.25 QRR130 pKa = 11.84 WFKK133 pKa = 10.13 WQLQSGLSRR142 pKa = 11.84 YY143 pKa = 8.4 ARR145 pKa = 11.84 PMYY148 pKa = 8.75 WQIGQLHH155 pKa = 6.12 QDD157 pKa = 3.76 YY158 pKa = 11.0 LPGSVRR164 pKa = 11.84 NLADD168 pKa = 3.2 TSLTDD173 pKa = 3.55 DD174 pKa = 4.45 CASITAVYY182 pKa = 10.49 DD183 pKa = 3.58 GANTSGPSAAPITVAADD200 pKa = 3.18 ASDD203 pKa = 3.55 PRR205 pKa = 11.84 GLFQVGHH212 pKa = 7.01 RR213 pKa = 11.84 GRR215 pKa = 11.84 LGWMPTDD222 pKa = 3.53 GVLLKK227 pKa = 9.68 GTSGGTGTVHH237 pKa = 6.14 AQAVEE242 pKa = 3.92 AAIPAVNVFTVVTPPMHH259 pKa = 5.75 KK260 pKa = 8.32 TNYY263 pKa = 7.64 YY264 pKa = 8.29 YY265 pKa = 10.56 RR266 pKa = 11.84 VFVTEE271 pKa = 4.34 DD272 pKa = 3.84 VYY274 pKa = 11.28 FKK276 pKa = 11.2 SPVVVGYY283 pKa = 9.58 QNYY286 pKa = 9.62 RR287 pKa = 11.84 SIDD290 pKa = 3.52 RR291 pKa = 11.84 FVQPQFPVAKK301 pKa = 9.25 LPTVSSPATDD311 pKa = 3.34 SNKK314 pKa = 9.71 PFPTNDD320 pKa = 3.36 GEE322 pKa = 4.31 QLPEE326 pKa = 3.82 VLII329 pKa = 4.48
Molecular weight: 36.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.247
IPC2_protein 6.186
IPC_protein 6.376
Toseland 6.825
ProMoST 6.722
Dawson 6.707
Bjellqvist 6.605
Wikipedia 6.737
Rodwell 6.693
Grimsley 6.985
Solomon 6.722
Lehninger 6.722
Nozaki 6.912
DTASelect 7.132
Thurlkill 7.146
EMBOSS 7.161
Sillero 7.073
Patrickios 4.012
IPC_peptide 6.737
IPC2_peptide 6.825
IPC2.peptide.svr19 6.9
Protein with the highest isoelectric point:
>tr|A0A0B5GST2|A0A0B5GST2_9VIRU Rep protein OS=Gorilla smacovirus OX=1603068 GN=rep PE=4 SV=1
MM1 pKa = 6.62 TQTWMLTVPRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.18 TEE15 pKa = 4.0 KK16 pKa = 9.83 EE17 pKa = 3.46 WMGIYY22 pKa = 9.84 KK23 pKa = 9.73 WLRR26 pKa = 11.84 DD27 pKa = 3.58 NDD29 pKa = 3.68 VHH31 pKa = 6.67 KK32 pKa = 9.25 WTVAMEE38 pKa = 4.34 TGSNGYY44 pKa = 8.07 DD45 pKa = 2.53 HH46 pKa = 6.6 WQIRR50 pKa = 11.84 FQVGKK55 pKa = 7.81 TFKK58 pKa = 10.19 QLKK61 pKa = 9.63 KK62 pKa = 8.91 EE63 pKa = 4.17 WGPKK67 pKa = 9.16 AHH69 pKa = 6.94 IEE71 pKa = 4.12 EE72 pKa = 5.48 ASDD75 pKa = 2.77 TWEE78 pKa = 4.11 YY79 pKa = 10.5 EE80 pKa = 4.14 RR81 pKa = 11.84 KK82 pKa = 10.05 SGMFFSSDD90 pKa = 3.26 DD91 pKa = 3.56 TPEE94 pKa = 3.65 VRR96 pKa = 11.84 KK97 pKa = 10.25 CRR99 pKa = 11.84 FGRR102 pKa = 11.84 LNWRR106 pKa = 11.84 QRR108 pKa = 11.84 TIVRR112 pKa = 11.84 AVQDD116 pKa = 3.64 TNDD119 pKa = 3.5 RR120 pKa = 11.84 EE121 pKa = 4.28 IVVWYY126 pKa = 10.2 DD127 pKa = 3.04 PDD129 pKa = 4.09 GNKK132 pKa = 10.05 GKK134 pKa = 10.18 SWLLGHH140 pKa = 7.53 LYY142 pKa = 8.31 EE143 pKa = 5.31 TGQAWVIQAQDD154 pKa = 3.46 TVKK157 pKa = 11.18 GIIQDD162 pKa = 3.87 CASEE166 pKa = 4.62 FINHH170 pKa = 5.19 GWRR173 pKa = 11.84 PIVVIDD179 pKa = 5.32 IPRR182 pKa = 11.84 TWKK185 pKa = 7.87 WTSDD189 pKa = 3.27 LYY191 pKa = 11.39 VAIEE195 pKa = 4.27 RR196 pKa = 11.84 IKK198 pKa = 11.05 DD199 pKa = 3.64 GLIKK203 pKa = 10.46 DD204 pKa = 3.5 PRR206 pKa = 11.84 YY207 pKa = 10.1 NSKK210 pKa = 8.3 TVHH213 pKa = 4.97 IRR215 pKa = 11.84 GVKK218 pKa = 9.98 VLITCNTMPKK228 pKa = 9.15 RR229 pKa = 11.84 DD230 pKa = 4.07 KK231 pKa = 11.05 LSADD235 pKa = 2.65 RR236 pKa = 11.84 WVIVEE241 pKa = 4.01 LL242 pKa = 4.3
Molecular weight: 28.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.792
IPC2_protein 8.624
IPC_protein 8.551
Toseland 9.37
ProMoST 9.077
Dawson 9.604
Bjellqvist 9.326
Wikipedia 9.75
Rodwell 9.94
Grimsley 9.663
Solomon 9.663
Lehninger 9.633
Nozaki 9.502
DTASelect 9.282
Thurlkill 9.472
EMBOSS 9.794
Sillero 9.575
Patrickios 5.385
IPC_peptide 9.663
IPC2_peptide 7.892
IPC2.peptide.svr19 7.802
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
571
242
329
285.5
32.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.604 ± 1.092
0.701 ± 0.312
6.83 ± 0.352
4.378 ± 0.815
3.678 ± 0.694
7.531 ± 0.772
2.102 ± 0.219
4.904 ± 1.468
6.305 ± 1.375
6.305 ± 0.78
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.977 ± 0.288
2.977 ± 0.19
5.429 ± 1.23
5.254 ± 0.65
6.13 ± 0.997
5.079 ± 0.548
7.706 ± 0.155
8.231 ± 0.22
3.503 ± 1.562
4.378 ± 0.861
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here