Amino acid dipepetide frequency for Escherichia phage JN02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.074AlaAla: 5.074 ± 0.455
0.419AlaCys: 0.419 ± 0.091
3.926AlaAsp: 3.926 ± 0.276
4.875AlaGlu: 4.875 ± 0.388
2.316AlaPhe: 2.316 ± 0.193
4.434AlaGly: 4.434 ± 0.366
1.103AlaHis: 1.103 ± 0.155
5.184AlaIle: 5.184 ± 0.341
4.941AlaLys: 4.941 ± 0.358
6.066AlaLeu: 6.066 ± 0.372
1.478AlaMet: 1.478 ± 0.184
3.662AlaAsn: 3.662 ± 0.307
2.581AlaPro: 2.581 ± 0.297
2.801AlaGln: 2.801 ± 0.281
3.044AlaArg: 3.044 ± 0.256
4.831AlaSer: 4.831 ± 0.334
3.949AlaThr: 3.949 ± 0.53
4.279AlaVal: 4.279 ± 0.296
0.993AlaTrp: 0.993 ± 0.137
2.559AlaTyr: 2.559 ± 0.232
0.0AlaXaa: 0.0 ± 0.0
Cys
0.706CysAla: 0.706 ± 0.125
0.221CysCys: 0.221 ± 0.091
0.441CysAsp: 0.441 ± 0.089
0.75CysGlu: 0.75 ± 0.134
0.441CysPhe: 0.441 ± 0.1
0.772CysGly: 0.772 ± 0.138
0.265CysHis: 0.265 ± 0.075
0.662CysIle: 0.662 ± 0.108
0.662CysLys: 0.662 ± 0.156
0.596CysLeu: 0.596 ± 0.139
0.243CysMet: 0.243 ± 0.085
0.596CysAsn: 0.596 ± 0.117
0.574CysPro: 0.574 ± 0.15
0.331CysGln: 0.331 ± 0.092
0.772CysArg: 0.772 ± 0.153
0.706CysSer: 0.706 ± 0.114
0.507CysThr: 0.507 ± 0.1
0.574CysVal: 0.574 ± 0.11
0.088CysTrp: 0.088 ± 0.047
0.419CysTyr: 0.419 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
4.699AspAla: 4.699 ± 0.339
0.618AspCys: 0.618 ± 0.106
3.838AspAsp: 3.838 ± 0.27
4.302AspGlu: 4.302 ± 0.376
3.353AspPhe: 3.353 ± 0.31
5.206AspGly: 5.206 ± 0.326
0.86AspHis: 0.86 ± 0.154
4.897AspIle: 4.897 ± 0.28
3.706AspLys: 3.706 ± 0.305
4.721AspLeu: 4.721 ± 0.363
1.588AspMet: 1.588 ± 0.199
2.868AspAsn: 2.868 ± 0.246
2.162AspPro: 2.162 ± 0.253
1.456AspGln: 1.456 ± 0.158
2.36AspArg: 2.36 ± 0.295
3.75AspSer: 3.75 ± 0.311
3.309AspThr: 3.309 ± 0.33
4.434AspVal: 4.434 ± 0.342
1.169AspTrp: 1.169 ± 0.188
3.11AspTyr: 3.11 ± 0.277
0.0AspXaa: 0.0 ± 0.0
Glu
5.162GluAla: 5.162 ± 0.368
1.015GluCys: 1.015 ± 0.161
3.993GluAsp: 3.993 ± 0.321
4.434GluGlu: 4.434 ± 0.39
3.662GluPhe: 3.662 ± 0.283
3.772GluGly: 3.772 ± 0.319
1.191GluHis: 1.191 ± 0.202
6.044GluIle: 6.044 ± 0.485
4.61GluLys: 4.61 ± 0.375
6.309GluLeu: 6.309 ± 0.419
1.963GluMet: 1.963 ± 0.185
3.662GluAsn: 3.662 ± 0.323
1.831GluPro: 1.831 ± 0.228
2.36GluGln: 2.36 ± 0.248
2.735GluArg: 2.735 ± 0.262
3.794GluSer: 3.794 ± 0.307
4.213GluThr: 4.213 ± 0.366
4.831GluVal: 4.831 ± 0.359
0.838GluTrp: 0.838 ± 0.14
3.11GluTyr: 3.11 ± 0.265
0.0GluXaa: 0.0 ± 0.0
Phe
2.89PheAla: 2.89 ± 0.276
0.397PheCys: 0.397 ± 0.102
3.243PheAsp: 3.243 ± 0.274
3.662PheGlu: 3.662 ± 0.288
1.235PhePhe: 1.235 ± 0.172
2.691PheGly: 2.691 ± 0.211
0.684PheHis: 0.684 ± 0.129
2.956PheIle: 2.956 ± 0.222
3.838PheLys: 3.838 ± 0.318
2.36PheLeu: 2.36 ± 0.205
1.103PheMet: 1.103 ± 0.159
2.934PheAsn: 2.934 ± 0.277
1.081PhePro: 1.081 ± 0.168
1.257PheGln: 1.257 ± 0.188
1.875PheArg: 1.875 ± 0.221
3.0PheSer: 3.0 ± 0.214
2.713PheThr: 2.713 ± 0.23
2.934PheVal: 2.934 ± 0.255
0.574PheTrp: 0.574 ± 0.118
1.897PheTyr: 1.897 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
3.551GlyAla: 3.551 ± 0.417
0.485GlyCys: 0.485 ± 0.114
4.059GlyAsp: 4.059 ± 0.357
3.64GlyGlu: 3.64 ± 0.259
2.625GlyPhe: 2.625 ± 0.216
3.949GlyGly: 3.949 ± 0.385
0.904GlyHis: 0.904 ± 0.171
4.434GlyIle: 4.434 ± 0.31
4.279GlyLys: 4.279 ± 0.302
5.625GlyLeu: 5.625 ± 0.398
1.544GlyMet: 1.544 ± 0.231
3.529GlyAsn: 3.529 ± 0.49
1.831GlyPro: 1.831 ± 0.173
2.316GlyGln: 2.316 ± 0.264
2.846GlyArg: 2.846 ± 0.263
4.324GlySer: 4.324 ± 0.364
4.875GlyThr: 4.875 ± 0.48
4.213GlyVal: 4.213 ± 0.336
0.816GlyTrp: 0.816 ± 0.134
2.934GlyTyr: 2.934 ± 0.243
0.0GlyXaa: 0.0 ± 0.0
His
1.037HisAla: 1.037 ± 0.14
0.331HisCys: 0.331 ± 0.105
0.949HisAsp: 0.949 ± 0.138
1.015HisGlu: 1.015 ± 0.177
0.904HisPhe: 0.904 ± 0.14
1.059HisGly: 1.059 ± 0.145
0.353HisHis: 0.353 ± 0.094
1.478HisIle: 1.478 ± 0.169
1.103HisLys: 1.103 ± 0.154
1.235HisLeu: 1.235 ± 0.172
0.419HisMet: 0.419 ± 0.097
0.838HisAsn: 0.838 ± 0.143
0.794HisPro: 0.794 ± 0.136
0.618HisGln: 0.618 ± 0.112
0.838HisArg: 0.838 ± 0.186
1.125HisSer: 1.125 ± 0.202
0.926HisThr: 0.926 ± 0.174
0.993HisVal: 0.993 ± 0.159
0.199HisTrp: 0.199 ± 0.075
0.662HisTyr: 0.662 ± 0.129
0.0HisXaa: 0.0 ± 0.0
Ile
4.677IleAla: 4.677 ± 0.336
0.684IleCys: 0.684 ± 0.11
5.074IleAsp: 5.074 ± 0.364
5.272IleGlu: 5.272 ± 0.376
2.449IlePhe: 2.449 ± 0.23
4.257IleGly: 4.257 ± 0.291
1.081IleHis: 1.081 ± 0.162
4.677IleIle: 4.677 ± 0.364
6.904IleLys: 6.904 ± 0.525
4.434IleLeu: 4.434 ± 0.353
1.61IleMet: 1.61 ± 0.198
5.007IleAsn: 5.007 ± 0.347
2.824IlePro: 2.824 ± 0.279
2.537IleGln: 2.537 ± 0.221
3.132IleArg: 3.132 ± 0.274
4.5IleSer: 4.5 ± 0.303
5.074IleThr: 5.074 ± 0.364
4.632IleVal: 4.632 ± 0.366
0.551IleTrp: 0.551 ± 0.121
2.559IleTyr: 2.559 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
5.669LysAla: 5.669 ± 0.407
0.618LysCys: 0.618 ± 0.122
4.368LysAsp: 4.368 ± 0.317
5.735LysGlu: 5.735 ± 0.495
3.485LysPhe: 3.485 ± 0.308
4.103LysGly: 4.103 ± 0.285
1.588LysHis: 1.588 ± 0.21
5.118LysIle: 5.118 ± 0.387
4.61LysLys: 4.61 ± 0.407
6.088LysLeu: 6.088 ± 0.421
2.118LysMet: 2.118 ± 0.222
4.302LysAsn: 4.302 ± 0.33
2.647LysPro: 2.647 ± 0.251
2.338LysGln: 2.338 ± 0.229
2.89LysArg: 2.89 ± 0.245
4.213LysSer: 4.213 ± 0.345
4.478LysThr: 4.478 ± 0.389
4.721LysVal: 4.721 ± 0.335
1.213LysTrp: 1.213 ± 0.152
3.375LysTyr: 3.375 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
5.559LeuAla: 5.559 ± 0.398
0.904LeuCys: 0.904 ± 0.157
5.36LeuAsp: 5.36 ± 0.363
5.029LeuGlu: 5.029 ± 0.366
3.287LeuPhe: 3.287 ± 0.302
4.125LeuGly: 4.125 ± 0.285
1.191LeuHis: 1.191 ± 0.148
4.434LeuIle: 4.434 ± 0.322
5.713LeuLys: 5.713 ± 0.426
4.743LeuLeu: 4.743 ± 0.37
2.162LeuMet: 2.162 ± 0.253
4.765LeuAsn: 4.765 ± 0.353
3.199LeuPro: 3.199 ± 0.262
2.824LeuGln: 2.824 ± 0.23
3.507LeuArg: 3.507 ± 0.214
4.743LeuSer: 4.743 ± 0.33
4.677LeuThr: 4.677 ± 0.379
4.324LeuVal: 4.324 ± 0.323
0.684LeuTrp: 0.684 ± 0.145
3.221LeuTyr: 3.221 ± 0.249
0.0LeuXaa: 0.0 ± 0.0
Met
2.25MetAla: 2.25 ± 0.23
0.353MetCys: 0.353 ± 0.091
1.257MetAsp: 1.257 ± 0.153
1.831MetGlu: 1.831 ± 0.218
1.081MetPhe: 1.081 ± 0.153
1.279MetGly: 1.279 ± 0.187
0.265MetHis: 0.265 ± 0.074
1.434MetIle: 1.434 ± 0.171
2.184MetLys: 2.184 ± 0.284
2.162MetLeu: 2.162 ± 0.2
0.904MetMet: 0.904 ± 0.164
1.676MetAsn: 1.676 ± 0.189
0.728MetPro: 0.728 ± 0.132
0.772MetGln: 0.772 ± 0.125
1.015MetArg: 1.015 ± 0.128
1.941MetSer: 1.941 ± 0.203
1.699MetThr: 1.699 ± 0.192
1.566MetVal: 1.566 ± 0.189
0.221MetTrp: 0.221 ± 0.074
0.882MetTyr: 0.882 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
3.772AsnAla: 3.772 ± 0.336
0.485AsnCys: 0.485 ± 0.117
2.912AsnAsp: 2.912 ± 0.258
3.993AsnGlu: 3.993 ± 0.255
2.779AsnPhe: 2.779 ± 0.211
4.037AsnGly: 4.037 ± 0.382
1.015AsnHis: 1.015 ± 0.14
4.235AsnIle: 4.235 ± 0.307
4.213AsnLys: 4.213 ± 0.348
4.235AsnLeu: 4.235 ± 0.322
1.676AsnMet: 1.676 ± 0.18
3.485AsnAsn: 3.485 ± 0.329
2.669AsnPro: 2.669 ± 0.272
1.5AsnGln: 1.5 ± 0.222
2.338AsnArg: 2.338 ± 0.198
3.728AsnSer: 3.728 ± 0.312
3.375AsnThr: 3.375 ± 0.278
3.618AsnVal: 3.618 ± 0.309
0.662AsnTrp: 0.662 ± 0.111
2.14AsnTyr: 2.14 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
2.25ProAla: 2.25 ± 0.24
0.397ProCys: 0.397 ± 0.091
2.471ProAsp: 2.471 ± 0.21
3.375ProGlu: 3.375 ± 0.351
1.743ProPhe: 1.743 ± 0.211
2.36ProGly: 2.36 ± 0.245
0.596ProHis: 0.596 ± 0.129
2.118ProIle: 2.118 ± 0.217
2.669ProLys: 2.669 ± 0.254
2.162ProLeu: 2.162 ± 0.216
0.684ProMet: 0.684 ± 0.12
2.184ProAsn: 2.184 ± 0.215
0.993ProPro: 0.993 ± 0.171
1.147ProGln: 1.147 ± 0.16
1.522ProArg: 1.522 ± 0.203
2.449ProSer: 2.449 ± 0.238
2.449ProThr: 2.449 ± 0.261
3.022ProVal: 3.022 ± 0.221
0.728ProTrp: 0.728 ± 0.125
1.324ProTyr: 1.324 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
2.449GlnAla: 2.449 ± 0.287
0.309GlnCys: 0.309 ± 0.076
1.765GlnAsp: 1.765 ± 0.211
2.294GlnGlu: 2.294 ± 0.212
1.699GlnPhe: 1.699 ± 0.202
2.096GlnGly: 2.096 ± 0.188
0.551GlnHis: 0.551 ± 0.113
2.934GlnIle: 2.934 ± 0.221
2.426GlnLys: 2.426 ± 0.224
2.603GlnLeu: 2.603 ± 0.223
1.037GlnMet: 1.037 ± 0.164
1.588GlnAsn: 1.588 ± 0.172
1.081GlnPro: 1.081 ± 0.149
1.059GlnGln: 1.059 ± 0.164
1.676GlnArg: 1.676 ± 0.203
1.522GlnSer: 1.522 ± 0.189
1.963GlnThr: 1.963 ± 0.256
2.713GlnVal: 2.713 ± 0.275
0.75GlnTrp: 0.75 ± 0.119
1.61GlnTyr: 1.61 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
2.801ArgAla: 2.801 ± 0.263
0.529ArgCys: 0.529 ± 0.113
2.426ArgAsp: 2.426 ± 0.212
3.11ArgGlu: 3.11 ± 0.277
1.875ArgPhe: 1.875 ± 0.211
2.824ArgGly: 2.824 ± 0.294
0.706ArgHis: 0.706 ± 0.135
3.397ArgIle: 3.397 ± 0.279
3.331ArgLys: 3.331 ± 0.315
3.507ArgLeu: 3.507 ± 0.258
1.081ArgMet: 1.081 ± 0.159
2.14ArgAsn: 2.14 ± 0.215
1.456ArgPro: 1.456 ± 0.182
1.699ArgGln: 1.699 ± 0.179
2.206ArgArg: 2.206 ± 0.203
2.824ArgSer: 2.824 ± 0.241
2.493ArgThr: 2.493 ± 0.216
3.0ArgVal: 3.0 ± 0.266
0.441ArgTrp: 0.441 ± 0.1
1.809ArgTyr: 1.809 ± 0.202
0.0ArgXaa: 0.0 ± 0.0
Ser
3.507SerAla: 3.507 ± 0.325
0.551SerCys: 0.551 ± 0.133
3.926SerAsp: 3.926 ± 0.33
3.949SerGlu: 3.949 ± 0.299
2.691SerPhe: 2.691 ± 0.246
4.632SerGly: 4.632 ± 0.366
1.103SerHis: 1.103 ± 0.183
4.919SerIle: 4.919 ± 0.274
4.721SerLys: 4.721 ± 0.349
4.853SerLeu: 4.853 ± 0.283
1.434SerMet: 1.434 ± 0.18
3.221SerAsn: 3.221 ± 0.275
2.493SerPro: 2.493 ± 0.23
2.382SerGln: 2.382 ± 0.24
2.934SerArg: 2.934 ± 0.267
4.831SerSer: 4.831 ± 0.408
4.412SerThr: 4.412 ± 0.352
3.993SerVal: 3.993 ± 0.328
0.882SerTrp: 0.882 ± 0.158
2.846SerTyr: 2.846 ± 0.225
0.0SerXaa: 0.0 ± 0.0
Thr
4.235ThrAla: 4.235 ± 0.38
0.551ThrCys: 0.551 ± 0.123
4.169ThrAsp: 4.169 ± 0.346
3.926ThrGlu: 3.926 ± 0.291
2.426ThrPhe: 2.426 ± 0.226
4.478ThrGly: 4.478 ± 0.457
1.368ThrHis: 1.368 ± 0.195
4.434ThrIle: 4.434 ± 0.397
4.125ThrLys: 4.125 ± 0.308
4.279ThrLeu: 4.279 ± 0.367
1.081ThrMet: 1.081 ± 0.173
2.978ThrAsn: 2.978 ± 0.248
3.265ThrPro: 3.265 ± 0.318
2.096ThrGln: 2.096 ± 0.268
2.669ThrArg: 2.669 ± 0.314
4.015ThrSer: 4.015 ± 0.401
3.75ThrThr: 3.75 ± 0.337
4.765ThrVal: 4.765 ± 0.502
0.993ThrTrp: 0.993 ± 0.169
2.647ThrTyr: 2.647 ± 0.249
0.0ThrXaa: 0.0 ± 0.0
Val
4.456ValAla: 4.456 ± 0.342
0.794ValCys: 0.794 ± 0.123
4.699ValAsp: 4.699 ± 0.318
5.074ValGlu: 5.074 ± 0.394
2.713ValPhe: 2.713 ± 0.283
3.706ValGly: 3.706 ± 0.353
1.059ValHis: 1.059 ± 0.142
4.654ValIle: 4.654 ± 0.289
5.338ValLys: 5.338 ± 0.309
4.765ValLeu: 4.765 ± 0.37
1.654ValMet: 1.654 ± 0.228
3.794ValAsn: 3.794 ± 0.346
2.294ValPro: 2.294 ± 0.186
2.493ValGln: 2.493 ± 0.235
3.199ValArg: 3.199 ± 0.265
4.61ValSer: 4.61 ± 0.308
4.037ValThr: 4.037 ± 0.372
4.456ValVal: 4.456 ± 0.285
0.816ValTrp: 0.816 ± 0.132
2.603ValTyr: 2.603 ± 0.212
0.0ValXaa: 0.0 ± 0.0
Trp
0.794TrpAla: 0.794 ± 0.125
0.154TrpCys: 0.154 ± 0.061
0.75TrpAsp: 0.75 ± 0.133
0.728TrpGlu: 0.728 ± 0.118
0.662TrpPhe: 0.662 ± 0.121
0.574TrpGly: 0.574 ± 0.122
0.154TrpHis: 0.154 ± 0.057
0.949TrpIle: 0.949 ± 0.162
1.346TrpLys: 1.346 ± 0.176
0.816TrpLeu: 0.816 ± 0.136
0.485TrpMet: 0.485 ± 0.111
1.015TrpAsn: 1.015 ± 0.145
0.441TrpPro: 0.441 ± 0.108
0.529TrpGln: 0.529 ± 0.103
0.529TrpArg: 0.529 ± 0.096
0.75TrpSer: 0.75 ± 0.117
0.86TrpThr: 0.86 ± 0.126
0.949TrpVal: 0.949 ± 0.144
0.176TrpTrp: 0.176 ± 0.057
0.75TrpTyr: 0.75 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.978TyrAla: 2.978 ± 0.231
0.507TyrCys: 0.507 ± 0.121
2.779TyrAsp: 2.779 ± 0.231
2.537TyrGlu: 2.537 ± 0.305
1.919TyrPhe: 1.919 ± 0.185
2.426TyrGly: 2.426 ± 0.242
0.838TyrHis: 0.838 ± 0.131
2.978TyrIle: 2.978 ± 0.267
2.956TyrLys: 2.956 ± 0.274
2.89TyrLeu: 2.89 ± 0.237
1.213TyrMet: 1.213 ± 0.152
2.625TyrAsn: 2.625 ± 0.242
1.699TyrPro: 1.699 ± 0.189
1.61TyrGln: 1.61 ± 0.206
1.566TyrArg: 1.566 ± 0.186
2.625TyrSer: 2.625 ± 0.261
2.493TyrThr: 2.493 ± 0.249
3.199TyrVal: 3.199 ± 0.291
0.618TyrTrp: 0.618 ± 0.129
1.787TyrTyr: 1.787 ± 0.222
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 172 proteins (45334 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski