Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria; Listeria monocytogenes

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8Y5S7|Q8Y5S7_LISMO Glucose-6-phosphate 1-dehydrogenase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=zwf PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.35KK3 pKa = 9.79ILTGLSTTLLLTVLLLTITGGDD25 pKa = 3.84LKK27 pKa = 11.27AKK29 pKa = 9.85AASDD33 pKa = 4.66LYY35 pKa = 10.17PLPAPIIDD43 pKa = 3.65VFPDD47 pKa = 2.87EE48 pKa = 5.43GLAKK52 pKa = 10.8DD53 pKa = 3.47MAKK56 pKa = 10.48NLNKK60 pKa = 10.62DD61 pKa = 3.45SVNDD65 pKa = 4.51VIDD68 pKa = 4.12QDD70 pKa = 5.27DD71 pKa = 4.27LDD73 pKa = 4.76ALTGLGFEE81 pKa = 5.06TEE83 pKa = 4.53TITNDD88 pKa = 3.19SMQLLEE94 pKa = 4.23RR95 pKa = 11.84AMFNNVNIVSVMEE108 pKa = 4.23FGEE111 pKa = 4.6DD112 pKa = 3.13LTEE115 pKa = 4.73FPDD118 pKa = 3.76ISTIPHH124 pKa = 7.06LNTLFFNTPPEE135 pKa = 4.32GVTRR139 pKa = 11.84NLSLPDD145 pKa = 3.48YY146 pKa = 10.41QNYY149 pKa = 9.91PEE151 pKa = 4.57MVTITMSGSNLIGAIPDD168 pKa = 3.71FTGMPDD174 pKa = 4.03LSQLYY179 pKa = 8.27MADD182 pKa = 3.42MMITSDD188 pKa = 4.94DD189 pKa = 3.69VPDD192 pKa = 3.89FHH194 pKa = 7.19TIPKK198 pKa = 10.39LSTLDD203 pKa = 3.7LSHH206 pKa = 6.73NQLTNLPDD214 pKa = 3.92FQNLTNLAEE223 pKa = 4.65LNLSFNNLTNTMTNFTNLSNLNNLNLDD250 pKa = 3.9YY251 pKa = 11.52NHH253 pKa = 7.05LNEE256 pKa = 5.25LPSNVLNSIFIEE268 pKa = 4.34NQSGTVPDD276 pKa = 3.91QIIKK280 pKa = 10.44QGEE283 pKa = 4.39TCTIQLPIYY292 pKa = 9.44FQLAEE297 pKa = 4.22INMLVNPTVLGSYY310 pKa = 9.97SADD313 pKa = 3.0IPVEE317 pKa = 4.2VVTTTNADD325 pKa = 3.73TEE327 pKa = 5.01SITLDD332 pKa = 3.36TSEE335 pKa = 5.2LSPGVYY341 pKa = 10.18NFNVQFNDD349 pKa = 3.92AYY351 pKa = 10.13PITQEE356 pKa = 3.76GCVYY360 pKa = 10.99DD361 pKa = 3.5WVLTVNN367 pKa = 4.06

Molecular weight: 40.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7AP96|Q7AP96_LISMO LmaD protein OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=lmaD PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.59RR10 pKa = 11.84KK11 pKa = 9.09RR12 pKa = 11.84KK13 pKa = 8.86KK14 pKa = 8.28VHH16 pKa = 5.49GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 9.32NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2844

0

2844

870843

28

2044

306.2

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.737 ± 0.051

0.609 ± 0.013

5.445 ± 0.048

7.439 ± 0.06

4.531 ± 0.039

6.671 ± 0.046

1.779 ± 0.019

7.84 ± 0.053

7.163 ± 0.04

9.506 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.022

4.621 ± 0.036

3.472 ± 0.027

3.457 ± 0.029

3.652 ± 0.038

5.799 ± 0.033

6.114 ± 0.055

7.041 ± 0.04

0.93 ± 0.018

3.448 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski