Paraburkholderia xenovorans (strain LB400)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; Paraburkholderia xenovorans

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8591 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q13JQ5|Q13JQ5_PARXL Uncharacterized protein OS=Paraburkholderia xenovorans (strain LB400) OX=266265 GN=Bxe_B0262 PE=4 SV=1
MM1 pKa = 7.39SLDD4 pKa = 3.7EE5 pKa = 4.99SPAPHH10 pKa = 7.06APDD13 pKa = 3.93AQPAGASSAATADD26 pKa = 4.58DD27 pKa = 4.53FKK29 pKa = 11.45QWVCVICGWVYY40 pKa = 11.39DD41 pKa = 4.39EE42 pKa = 5.39AAGLPEE48 pKa = 4.46EE49 pKa = 5.49GIAPGTRR56 pKa = 11.84WADD59 pKa = 3.21IPPDD63 pKa = 3.75WRR65 pKa = 11.84CPLCDD70 pKa = 3.32VGKK73 pKa = 10.7EE74 pKa = 3.91DD75 pKa = 3.78FALVEE80 pKa = 4.41FF81 pKa = 4.99

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q13SH8|Q13SH8_PARXL Putative phage integrase OS=Paraburkholderia xenovorans (strain LB400) OX=266265 GN=Bxe_A4470 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8591

0

8591

2754203

30

4726

320.6

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.605 ± 0.035

0.972 ± 0.008

5.292 ± 0.021

5.086 ± 0.025

3.698 ± 0.018

8.276 ± 0.029

2.332 ± 0.013

4.713 ± 0.018

2.985 ± 0.021

10.159 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.012

2.887 ± 0.029

5.097 ± 0.024

3.643 ± 0.016

7.062 ± 0.029

5.855 ± 0.024

5.489 ± 0.028

7.702 ± 0.021

1.365 ± 0.01

2.444 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski