Amino acid dipepetide frequency for Eubacterium pyruvativorans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.646AlaAla: 9.646 ± 0.175	1.225AlaCys: 1.225 ± 0.058	5.142AlaAsp: 5.142 ± 0.093	6.798AlaGlu: 6.798 ± 0.127	3.372AlaPhe: 3.372 ± 0.092	7.984AlaGly: 7.984 ± 0.126	1.28AlaHis: 1.28 ± 0.048	4.301AlaIle: 4.301 ± 0.095	4.592AlaLys: 4.592 ± 0.117	7.497AlaLeu: 7.497 ± 0.123	2.639AlaMet: 2.639 ± 0.069	2.232AlaAsn: 2.232 ± 0.06	2.646AlaPro: 2.646 ± 0.064	2.053AlaGln: 2.053 ± 0.075	4.358AlaArg: 4.358 ± 0.093	4.333AlaSer: 4.333 ± 0.094	2.944AlaThr: 2.944 ± 0.09	7.179AlaVal: 7.179 ± 0.127	0.66AlaTrp: 0.66 ± 0.035	2.577AlaTyr: 2.577 ± 0.072	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.044	0.315CysCys: 0.315 ± 0.023	0.759CysAsp: 0.759 ± 0.036	0.785CysGlu: 0.785 ± 0.035	0.556CysPhe: 0.556 ± 0.028	1.651CysGly: 1.651 ± 0.064	0.3CysHis: 0.3 ± 0.021	0.896CysIle: 0.896 ± 0.041	0.423CysLys: 0.423 ± 0.026	1.041CysLeu: 1.041 ± 0.043	0.444CysMet: 0.444 ± 0.027	0.417CysAsn: 0.417 ± 0.026	0.739CysPro: 0.739 ± 0.036	0.296CysGln: 0.296 ± 0.02	1.055CysArg: 1.055 ± 0.046	0.827CysSer: 0.827 ± 0.037	0.754CysThr: 0.754 ± 0.037	0.958CysVal: 0.958 ± 0.037	0.128CysTrp: 0.128 ± 0.014	0.453CysTyr: 0.453 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.856AspAla: 4.856 ± 0.086	0.786AspCys: 0.786 ± 0.037	2.915AspAsp: 2.915 ± 0.071	4.399AspGlu: 4.399 ± 0.103	2.621AspPhe: 2.621 ± 0.066	4.642AspGly: 4.642 ± 0.093	1.374AspHis: 1.374 ± 0.052	3.949AspIle: 3.949 ± 0.084	2.287AspLys: 2.287 ± 0.063	5.543AspLeu: 5.543 ± 0.103	1.879AspMet: 1.879 ± 0.055	1.724AspAsn: 1.724 ± 0.056	3.162AspPro: 3.162 ± 0.071	1.803AspGln: 1.803 ± 0.062	4.359AspArg: 4.359 ± 0.09	2.831AspSer: 2.831 ± 0.072	2.89AspThr: 2.89 ± 0.067	4.061AspVal: 4.061 ± 0.084	0.594AspTrp: 0.594 ± 0.028	2.457AspTyr: 2.457 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	6.249GluAla: 6.249 ± 0.126	0.739GluCys: 0.739 ± 0.042	4.581GluAsp: 4.581 ± 0.096	7.356GluGlu: 7.356 ± 0.146	2.142GluPhe: 2.142 ± 0.059	4.678GluGly: 4.678 ± 0.086	1.396GluHis: 1.396 ± 0.054	5.101GluIle: 5.101 ± 0.11	5.94GluLys: 5.94 ± 0.105	6.247GluLeu: 6.247 ± 0.133	2.781GluMet: 2.781 ± 0.07	3.537GluAsn: 3.537 ± 0.078	2.133GluPro: 2.133 ± 0.064	2.842GluGln: 2.842 ± 0.078	4.055GluArg: 4.055 ± 0.105	3.185GluSer: 3.185 ± 0.076	4.006GluThr: 4.006 ± 0.08	4.318GluVal: 4.318 ± 0.087	0.605GluTrp: 0.605 ± 0.032	2.541GluTyr: 2.541 ± 0.07	0.0GluXaa: 0.0 ± 0.0
Phe	2.825PheAla: 2.825 ± 0.073	0.695PheCys: 0.695 ± 0.037	2.434PheAsp: 2.434 ± 0.065	2.195PheGlu: 2.195 ± 0.057	1.599PhePhe: 1.599 ± 0.055	3.351PheGly: 3.351 ± 0.078	0.889PheHis: 0.889 ± 0.037	2.165PheIle: 2.165 ± 0.063	1.245PheLys: 1.245 ± 0.056	3.803PheLeu: 3.803 ± 0.102	0.999PheMet: 0.999 ± 0.042	1.33PheAsn: 1.33 ± 0.053	1.612PhePro: 1.612 ± 0.061	1.101PheGln: 1.101 ± 0.044	3.214PheArg: 3.214 ± 0.076	2.459PheSer: 2.459 ± 0.067	2.284PheThr: 2.284 ± 0.073	2.626PheVal: 2.626 ± 0.066	0.385PheTrp: 0.385 ± 0.028	1.248PheTyr: 1.248 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	6.193GlyAla: 6.193 ± 0.135	1.236GlyCys: 1.236 ± 0.051	4.093GlyAsp: 4.093 ± 0.087	5.119GlyGlu: 5.119 ± 0.114	3.248GlyPhe: 3.248 ± 0.078	5.951GlyGly: 5.951 ± 0.152	1.463GlyHis: 1.463 ± 0.05	6.141GlyIle: 6.141 ± 0.111	5.331GlyLys: 5.331 ± 0.098	6.395GlyLeu: 6.395 ± 0.129	2.81GlyMet: 2.81 ± 0.068	2.831GlyAsn: 2.831 ± 0.075	1.975GlyPro: 1.975 ± 0.062	2.091GlyGln: 2.091 ± 0.055	4.759GlyArg: 4.759 ± 0.101	4.648GlySer: 4.648 ± 0.085	4.966GlyThr: 4.966 ± 0.095	5.449GlyVal: 5.449 ± 0.097	0.809GlyTrp: 0.809 ± 0.042	3.083GlyTyr: 3.083 ± 0.077	0.0GlyXaa: 0.0 ± 0.0
His	1.254HisAla: 1.254 ± 0.048	0.325HisCys: 0.325 ± 0.024	1.041HisAsp: 1.041 ± 0.044	1.194HisGlu: 1.194 ± 0.042	0.864HisPhe: 0.864 ± 0.046	1.539HisGly: 1.539 ± 0.052	0.552HisHis: 0.552 ± 0.036	1.294HisIle: 1.294 ± 0.048	0.585HisLys: 0.585 ± 0.027	1.552HisLeu: 1.552 ± 0.047	0.614HisMet: 0.614 ± 0.03	0.593HisAsn: 0.593 ± 0.033	1.107HisPro: 1.107 ± 0.04	0.576HisGln: 0.576 ± 0.03	1.232HisArg: 1.232 ± 0.045	1.055HisSer: 1.055 ± 0.042	0.982HisThr: 0.982 ± 0.039	1.373HisVal: 1.373 ± 0.046	0.173HisTrp: 0.173 ± 0.017	0.851HisTyr: 0.851 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	5.355IleAla: 5.355 ± 0.106	1.099IleCys: 1.099 ± 0.044	3.917IleAsp: 3.917 ± 0.091	3.695IleGlu: 3.695 ± 0.081	2.472IlePhe: 2.472 ± 0.077	4.868IleGly: 4.868 ± 0.102	1.327IleHis: 1.327 ± 0.049	3.997IleIle: 3.997 ± 0.098	1.858IleLys: 1.858 ± 0.057	6.361IleLeu: 6.361 ± 0.117	1.873IleMet: 1.873 ± 0.056	2.075IleAsn: 2.075 ± 0.065	3.143IlePro: 3.143 ± 0.065	1.825IleGln: 1.825 ± 0.053	5.516IleArg: 5.516 ± 0.107	4.19IleSer: 4.19 ± 0.079	4.022IleThr: 4.022 ± 0.069	4.593IleVal: 4.593 ± 0.084	0.54IleTrp: 0.54 ± 0.035	2.06IleTyr: 2.06 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	4.92LysAla: 4.92 ± 0.105	0.616LysCys: 0.616 ± 0.035	3.287LysAsp: 3.287 ± 0.079	4.727LysGlu: 4.727 ± 0.104	1.587LysPhe: 1.587 ± 0.051	3.596LysGly: 3.596 ± 0.086	0.897LysHis: 0.897 ± 0.037	3.619LysIle: 3.619 ± 0.083	4.911LysLys: 4.911 ± 0.107	4.73LysLeu: 4.73 ± 0.073	1.984LysMet: 1.984 ± 0.052	2.687LysAsn: 2.687 ± 0.075	2.106LysPro: 2.106 ± 0.066	1.934LysGln: 1.934 ± 0.061	2.959LysArg: 2.959 ± 0.06	2.954LysSer: 2.954 ± 0.075	3.388LysThr: 3.388 ± 0.076	3.736LysVal: 3.736 ± 0.098	0.488LysTrp: 0.488 ± 0.028	2.459LysTyr: 2.459 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	7.271LeuAla: 7.271 ± 0.112	1.16LeuCys: 1.16 ± 0.044	5.209LeuAsp: 5.209 ± 0.094	5.887LeuGlu: 5.887 ± 0.096	3.34LeuPhe: 3.34 ± 0.088	6.216LeuGly: 6.216 ± 0.12	1.639LeuHis: 1.639 ± 0.052	5.556LeuIle: 5.556 ± 0.117	5.189LeuLys: 5.189 ± 0.089	7.955LeuLeu: 7.955 ± 0.148	2.641LeuMet: 2.641 ± 0.071	3.26LeuAsn: 3.26 ± 0.071	3.702LeuPro: 3.702 ± 0.082	2.474LeuGln: 2.474 ± 0.065	5.487LeuArg: 5.487 ± 0.111	5.668LeuSer: 5.668 ± 0.108	5.41LeuThr: 5.41 ± 0.096	5.399LeuVal: 5.399 ± 0.101	0.654LeuTrp: 0.654 ± 0.032	2.775LeuTyr: 2.775 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.86MetAla: 2.86 ± 0.074	0.303MetCys: 0.303 ± 0.023	2.027MetAsp: 2.027 ± 0.058	2.445MetGlu: 2.445 ± 0.066	0.976MetPhe: 0.976 ± 0.035	2.325MetGly: 2.325 ± 0.068	0.509MetHis: 0.509 ± 0.031	2.133MetIle: 2.133 ± 0.063	2.49MetLys: 2.49 ± 0.058	2.565MetLeu: 2.565 ± 0.074	1.104MetMet: 1.104 ± 0.038	1.609MetAsn: 1.609 ± 0.049	1.336MetPro: 1.336 ± 0.049	1.01MetGln: 1.01 ± 0.038	1.662MetArg: 1.662 ± 0.053	1.701MetSer: 1.701 ± 0.052	2.042MetThr: 2.042 ± 0.05	1.855MetVal: 1.855 ± 0.054	0.189MetTrp: 0.189 ± 0.018	0.826MetTyr: 0.826 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.003AsnAla: 3.003 ± 0.062	0.532AsnCys: 0.532 ± 0.025	1.967AsnAsp: 1.967 ± 0.069	2.306AsnGlu: 2.306 ± 0.063	1.359AsnPhe: 1.359 ± 0.046	3.442AsnGly: 3.442 ± 0.086	0.804AsnHis: 0.804 ± 0.035	2.513AsnIle: 2.513 ± 0.062	1.443AsnLys: 1.443 ± 0.052	3.108AsnLeu: 3.108 ± 0.073	1.154AsnMet: 1.154 ± 0.041	1.291AsnAsn: 1.291 ± 0.055	1.846AsnPro: 1.846 ± 0.059	1.081AsnGln: 1.081 ± 0.048	2.632AsnArg: 2.632 ± 0.072	1.776AsnSer: 1.776 ± 0.059	1.999AsnThr: 1.999 ± 0.063	2.711AsnVal: 2.711 ± 0.066	0.392AsnTrp: 0.392 ± 0.024	1.417AsnTyr: 1.417 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.705ProAla: 3.705 ± 0.098	0.433ProCys: 0.433 ± 0.028	3.041ProAsp: 3.041 ± 0.065	4.513ProGlu: 4.513 ± 0.087	1.589ProPhe: 1.589 ± 0.048	3.383ProGly: 3.383 ± 0.092	0.608ProHis: 0.608 ± 0.028	1.765ProIle: 1.765 ± 0.059	2.013ProLys: 2.013 ± 0.069	2.924ProLeu: 2.924 ± 0.069	1.025ProMet: 1.025 ± 0.036	1.149ProAsn: 1.149 ± 0.043	0.952ProPro: 0.952 ± 0.041	0.926ProGln: 0.926 ± 0.039	1.568ProArg: 1.568 ± 0.047	2.021ProSer: 2.021 ± 0.059	1.548ProThr: 1.548 ± 0.059	3.596ProVal: 3.596 ± 0.082	0.307ProTrp: 0.307 ± 0.025	1.44ProTyr: 1.44 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	2.48GlnAla: 2.48 ± 0.072	0.318GlnCys: 0.318 ± 0.02	1.501GlnAsp: 1.501 ± 0.055	2.276GlnGlu: 2.276 ± 0.063	1.083GlnPhe: 1.083 ± 0.039	1.882GlnGly: 1.882 ± 0.048	0.502GlnHis: 0.502 ± 0.032	2.215GlnIle: 2.215 ± 0.057	2.337GlnLys: 2.337 ± 0.063	2.425GlnLeu: 2.425 ± 0.061	1.069GlnMet: 1.069 ± 0.043	1.32GlnAsn: 1.32 ± 0.047	0.938GlnPro: 0.938 ± 0.041	1.069GlnGln: 1.069 ± 0.045	1.54GlnArg: 1.54 ± 0.045	1.434GlnSer: 1.434 ± 0.044	1.492GlnThr: 1.492 ± 0.052	1.958GlnVal: 1.958 ± 0.049	0.21GlnTrp: 0.21 ± 0.021	1.107GlnTyr: 1.107 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	4.289ArgAla: 4.289 ± 0.085	0.803ArgCys: 0.803 ± 0.036	3.631ArgAsp: 3.631 ± 0.078	5.696ArgGlu: 5.696 ± 0.131	2.323ArgPhe: 2.323 ± 0.058	3.755ArgGly: 3.755 ± 0.086	1.145ArgHis: 1.145 ± 0.041	4.669ArgIle: 4.669 ± 0.098	4.716ArgLys: 4.716 ± 0.088	4.762ArgLeu: 4.762 ± 0.094	2.145ArgMet: 2.145 ± 0.057	2.632ArgAsn: 2.632 ± 0.061	2.074ArgPro: 2.074 ± 0.062	2.075ArgGln: 2.075 ± 0.058	4.435ArgArg: 4.435 ± 0.109	3.497ArgSer: 3.497 ± 0.08	3.348ArgThr: 3.348 ± 0.073	3.78ArgVal: 3.78 ± 0.079	0.569ArgTrp: 0.569 ± 0.034	2.352ArgTyr: 2.352 ± 0.066	0.0ArgXaa: 0.0 ± 0.0
Ser	4.718SerAla: 4.718 ± 0.101	0.759SerCys: 0.759 ± 0.033	3.342SerAsp: 3.342 ± 0.081	3.641SerGlu: 3.641 ± 0.076	2.344SerPhe: 2.344 ± 0.066	5.813SerGly: 5.813 ± 0.108	1.02SerHis: 1.02 ± 0.04	3.295SerIle: 3.295 ± 0.085	2.579SerLys: 2.579 ± 0.069	4.76SerLeu: 4.76 ± 0.092	1.806SerMet: 1.806 ± 0.054	1.677SerAsn: 1.677 ± 0.065	1.955SerPro: 1.955 ± 0.051	1.508SerGln: 1.508 ± 0.047	3.844SerArg: 3.844 ± 0.081	3.403SerSer: 3.403 ± 0.088	2.755SerThr: 2.755 ± 0.076	4.365SerVal: 4.365 ± 0.089	0.543SerTrp: 0.543 ± 0.03	1.815SerTyr: 1.815 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	4.903ThrAla: 4.903 ± 0.117	0.693ThrCys: 0.693 ± 0.038	3.435ThrAsp: 3.435 ± 0.072	4.049ThrGlu: 4.049 ± 0.088	2.121ThrPhe: 2.121 ± 0.051	5.6ThrGly: 5.6 ± 0.097	0.818ThrHis: 0.818 ± 0.033	3.427ThrIle: 3.427 ± 0.077	3.257ThrLys: 3.257 ± 0.08	4.587ThrLeu: 4.587 ± 0.088	1.447ThrMet: 1.447 ± 0.048	1.794ThrAsn: 1.794 ± 0.056	2.427ThrPro: 2.427 ± 0.073	1.168ThrGln: 1.168 ± 0.043	2.557ThrArg: 2.557 ± 0.07	2.65ThrSer: 2.65 ± 0.071	2.787ThrThr: 2.787 ± 0.108	4.757ThrVal: 4.757 ± 0.098	0.48ThrTrp: 0.48 ± 0.029	1.78ThrTyr: 1.78 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	4.663ValAla: 4.663 ± 0.095	1.18ValCys: 1.18 ± 0.048	3.876ValAsp: 3.876 ± 0.077	4.342ValGlu: 4.342 ± 0.082	2.985ValPhe: 2.985 ± 0.082	4.353ValGly: 4.353 ± 0.087	1.215ValHis: 1.215 ± 0.038	5.098ValIle: 5.098 ± 0.101	4.289ValLys: 4.289 ± 0.088	6.687ValLeu: 6.687 ± 0.116	2.323ValMet: 2.323 ± 0.067	2.804ValAsn: 2.804 ± 0.065	3.047ValPro: 3.047 ± 0.075	1.844ValGln: 1.844 ± 0.058	4.446ValArg: 4.446 ± 0.095	4.73ValSer: 4.73 ± 0.095	4.63ValThr: 4.63 ± 0.09	4.608ValVal: 4.608 ± 0.095	0.579ValTrp: 0.579 ± 0.029	2.389ValTyr: 2.389 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	0.569TrpAla: 0.569 ± 0.031	0.129TrpCys: 0.129 ± 0.013	0.503TrpAsp: 0.503 ± 0.026	0.573TrpGlu: 0.573 ± 0.035	0.377TrpPhe: 0.377 ± 0.027	0.652TrpGly: 0.652 ± 0.033	0.17TrpHis: 0.17 ± 0.017	0.587TrpIle: 0.587 ± 0.029	0.734TrpLys: 0.734 ± 0.034	0.778TrpLeu: 0.778 ± 0.03	0.295TrpMet: 0.295 ± 0.022	0.456TrpAsn: 0.456 ± 0.031	0.262TrpPro: 0.262 ± 0.019	0.321TrpGln: 0.321 ± 0.022	0.43TrpArg: 0.43 ± 0.026	0.467TrpSer: 0.467 ± 0.032	0.455TrpThr: 0.455 ± 0.029	0.5TrpVal: 0.5 ± 0.032	0.087TrpTrp: 0.087 ± 0.012	0.353TrpTyr: 0.353 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.614TyrAla: 2.614 ± 0.063	0.535TyrCys: 0.535 ± 0.031	2.434TyrAsp: 2.434 ± 0.068	2.437TyrGlu: 2.437 ± 0.061	1.499TyrPhe: 1.499 ± 0.05	2.909TyrGly: 2.909 ± 0.066	0.848TyrHis: 0.848 ± 0.036	2.066TyrIle: 2.066 ± 0.058	1.364TyrLys: 1.364 ± 0.049	3.169TyrLeu: 3.169 ± 0.083	0.931TyrMet: 0.931 ± 0.045	1.361TyrAsn: 1.361 ± 0.05	1.344TyrPro: 1.344 ± 0.05	1.159TyrGln: 1.159 ± 0.046	2.589TyrArg: 2.589 ± 0.068	2.115TyrSer: 2.115 ± 0.062	2.039TyrThr: 2.039 ± 0.061	2.264TyrVal: 2.264 ± 0.06	0.307TyrTrp: 0.307 ± 0.024	1.479TyrTyr: 1.479 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1994 proteins (657711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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