Antarctic penguin virus B
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0KCB8|A0A1Y0KCB8_9MONO Nucleocapsid OS=Antarctic penguin virus B OX=2006073 PE=3 SV=1
MM1 pKa = 7.79 ILRR4 pKa = 11.84 IITGRR9 pKa = 11.84 SRR11 pKa = 11.84 YY12 pKa = 9.59 SRR14 pKa = 11.84 GRR16 pKa = 11.84 DD17 pKa = 3.06 RR18 pKa = 11.84 EE19 pKa = 3.99 QTYY22 pKa = 10.68 SANHH26 pKa = 6.63 SILNMSSIFSEE37 pKa = 3.82 YY38 pKa = 11.04 DD39 pKa = 2.93 KK40 pKa = 11.19 FLEE43 pKa = 4.74 CQTHH47 pKa = 5.28 PVSRR51 pKa = 11.84 AMPTEE56 pKa = 4.19 GGGALKK62 pKa = 10.31 IEE64 pKa = 4.09 IPVFVLNSDD73 pKa = 3.78 DD74 pKa = 4.6 PEE76 pKa = 4.25 LRR78 pKa = 11.84 WRR80 pKa = 11.84 FTCFVLRR87 pKa = 11.84 LALSEE92 pKa = 4.29 SANRR96 pKa = 11.84 PLRR99 pKa = 11.84 QGALISVLCAHH110 pKa = 6.56 AQVMKK115 pKa = 10.03 TYY117 pKa = 10.81 VSLAAQSGEE126 pKa = 3.84 AVITILEE133 pKa = 4.12 IDD135 pKa = 4.18 DD136 pKa = 4.82 FEE138 pKa = 6.31 DD139 pKa = 4.88 KK140 pKa = 11.02 IPVFNNRR147 pKa = 11.84 SGITEE152 pKa = 3.82 EE153 pKa = 4.07 RR154 pKa = 11.84 ASRR157 pKa = 11.84 LAMIAEE163 pKa = 4.52 DD164 pKa = 4.8 LPRR167 pKa = 11.84 ACTNEE172 pKa = 4.05 TPFVNGNTEE181 pKa = 3.88 NDD183 pKa = 3.83 PPEE186 pKa = 4.67 DD187 pKa = 3.74 ASDD190 pKa = 3.5 VLDD193 pKa = 5.08 RR194 pKa = 11.84 IFSIQVQIWITVAKK208 pKa = 10.58 AMTAFEE214 pKa = 4.14 TAEE217 pKa = 3.87 EE218 pKa = 4.62 SEE220 pKa = 4.11 TRR222 pKa = 11.84 RR223 pKa = 11.84 LNKK226 pKa = 9.94 YY227 pKa = 6.6 MQQGRR232 pKa = 11.84 VQKK235 pKa = 10.47 KK236 pKa = 9.16 CLLFPIVRR244 pKa = 11.84 TTVQLTIRR252 pKa = 11.84 QSLLIRR258 pKa = 11.84 GFLVSEE264 pKa = 4.61 MKK266 pKa = 10.44 RR267 pKa = 11.84 AQNSPGGKK275 pKa = 7.92 SAYY278 pKa = 9.76 YY279 pKa = 10.66 SFVGDD284 pKa = 3.68 IAAYY288 pKa = 9.22 IKK290 pKa = 10.76 NAGVTAFLLTLKK302 pKa = 10.75 FGIQTRR308 pKa = 11.84 LPALALSSLSGDD320 pKa = 3.26 IQKK323 pKa = 10.15 VKK325 pKa = 10.84 QLMVLYY331 pKa = 10.29 RR332 pKa = 11.84 EE333 pKa = 4.3 KK334 pKa = 11.27 GEE336 pKa = 4.07 NGPYY340 pKa = 7.32 MTLLGDD346 pKa = 3.91 PDD348 pKa = 3.59 QMQFAPAEE356 pKa = 4.01 YY357 pKa = 10.29 SLLYY361 pKa = 10.23 SYY363 pKa = 11.85 AMGVASVLEE372 pKa = 4.44 SSTTRR377 pKa = 11.84 YY378 pKa = 9.55 QFARR382 pKa = 11.84 DD383 pKa = 3.6 FMNPTFWRR391 pKa = 11.84 IGVEE395 pKa = 4.19 SAQTLATSVDD405 pKa = 3.2 EE406 pKa = 4.91 GMASEE411 pKa = 4.4 LQLGKK416 pKa = 9.94 QSRR419 pKa = 11.84 AVLSEE424 pKa = 3.63 MMQKK428 pKa = 9.97 VAGSAGEE435 pKa = 4.06 YY436 pKa = 9.77 TMNAPAASVMVGSGVGEE453 pKa = 4.09 STRR456 pKa = 11.84 PLTSKK461 pKa = 10.39 VGSSRR466 pKa = 11.84 IQSDD470 pKa = 4.13 GAQIPPGFNTLEE482 pKa = 3.86 EE483 pKa = 4.7 YY484 pKa = 10.4 YY485 pKa = 10.71 DD486 pKa = 3.72 YY487 pKa = 10.73 MRR489 pKa = 11.84 SEE491 pKa = 4.69 GAGKK495 pKa = 10.36 AGTSAGTSSKK505 pKa = 10.58 PKK507 pKa = 10.3 TPGLDD512 pKa = 4.09 DD513 pKa = 4.35 DD514 pKa = 5.08 TDD516 pKa = 3.92 NQGDD520 pKa = 3.39 WSLL523 pKa = 3.62
Molecular weight: 57.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.37
IPC2_protein 5.537
IPC_protein 5.486
Toseland 5.448
ProMoST 5.626
Dawson 5.486
Bjellqvist 5.601
Wikipedia 5.359
Rodwell 5.41
Grimsley 5.397
Solomon 5.486
Lehninger 5.448
Nozaki 5.614
DTASelect 5.753
Thurlkill 5.486
EMBOSS 5.436
Sillero 5.703
Patrickios 4.253
IPC_peptide 5.499
IPC2_peptide 5.69
IPC2.peptide.svr19 5.773
Protein with the highest isoelectric point:
>tr|A0A1Y0KCB7|A0A1Y0KCB7_9MONO Phosphoprotein OS=Antarctic penguin virus B OX=2006073 PE=4 SV=1
MM1 pKa = 7.64 SLEE4 pKa = 4.03 SRR6 pKa = 11.84 TVRR9 pKa = 11.84 LYY11 pKa = 11.24 VDD13 pKa = 4.12 PDD15 pKa = 3.29 QAASSLLAFPLIFTSNEE32 pKa = 3.97 SGKK35 pKa = 8.68 KK36 pKa = 7.8 TLSPQYY42 pKa = 9.65 RR43 pKa = 11.84 IQIIEE48 pKa = 4.62 DD49 pKa = 3.55 DD50 pKa = 4.09 RR51 pKa = 11.84 EE52 pKa = 4.08 SDD54 pKa = 3.31 NDD56 pKa = 3.74 LVFISTYY63 pKa = 10.99 GFITGLEE70 pKa = 4.2 TAVDD74 pKa = 3.9 RR75 pKa = 11.84 SISVDD80 pKa = 3.14 MSQEE84 pKa = 3.95 RR85 pKa = 11.84 VVLTSCMLPLGSVPKK100 pKa = 10.47 TSDD103 pKa = 2.9 LHH105 pKa = 5.54 EE106 pKa = 4.38 LARR109 pKa = 11.84 ACLEE113 pKa = 4.34 LKK115 pKa = 9.8 VACKK119 pKa = 10.03 KK120 pKa = 10.53 AATNSEE126 pKa = 4.1 RR127 pKa = 11.84 IIFNILDD134 pKa = 4.39 APPVLAPCATIKK146 pKa = 10.63 QAVTSCAAAVNLKK159 pKa = 10.54 APEE162 pKa = 4.85 KK163 pKa = 10.88 IMGNYY168 pKa = 9.69 DD169 pKa = 3.97 LLYY172 pKa = 10.51 KK173 pKa = 9.68 VTFVSLTIIPAGSVYY188 pKa = 10.41 KK189 pKa = 10.83 VSSPVLKK196 pKa = 10.57 AGSSLTYY203 pKa = 10.65 GLNLSITLKK212 pKa = 10.28 IDD214 pKa = 3.22 IGDD217 pKa = 3.59 QHH219 pKa = 7.05 PSAKK223 pKa = 9.62 MLMKK227 pKa = 10.27 RR228 pKa = 11.84 DD229 pKa = 3.54 DD230 pKa = 3.74 GFYY233 pKa = 10.46 ANLWIHH239 pKa = 6.71 CGLLSAVKK247 pKa = 10.39 KK248 pKa = 10.44 GGKK251 pKa = 8.34 KK252 pKa = 9.94 HH253 pKa = 6.38 SIEE256 pKa = 4.29 EE257 pKa = 3.89 IANKK261 pKa = 8.9 VRR263 pKa = 11.84 RR264 pKa = 11.84 LEE266 pKa = 4.08 MKK268 pKa = 10.77 LMLVDD273 pKa = 3.47 MFGPSIVIKK282 pKa = 9.74 CTGVKK287 pKa = 9.26 TKK289 pKa = 10.64 LLAGFFSKK297 pKa = 10.41 KK298 pKa = 8.17 GTAVYY303 pKa = 9.07 PISRR307 pKa = 11.84 AAPGIGKK314 pKa = 8.82 LLWSQSGTITEE325 pKa = 4.0 AAIIIQGGTPHH336 pKa = 6.28 QLVSTSDD343 pKa = 3.65 YY344 pKa = 11.16 AVTSTKK350 pKa = 9.45 VTVGGGNSKK359 pKa = 10.86 YY360 pKa = 11.0 NPFKK364 pKa = 10.83 KK365 pKa = 10.46
Molecular weight: 39.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.687
IPC2_protein 8.536
IPC_protein 8.404
Toseland 9.151
ProMoST 9.033
Dawson 9.443
Bjellqvist 9.253
Wikipedia 9.575
Rodwell 9.75
Grimsley 9.458
Solomon 9.502
Lehninger 9.458
Nozaki 9.443
DTASelect 9.165
Thurlkill 9.326
EMBOSS 9.604
Sillero 9.472
Patrickios 4.838
IPC_peptide 9.487
IPC2_peptide 7.907
IPC2.peptide.svr19 7.786
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4621
365
2207
770.2
85.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.531 ± 0.536
2.056 ± 0.239
4.891 ± 0.477
4.696 ± 0.421
3.073 ± 0.346
5.865 ± 0.634
1.536 ± 0.413
6.709 ± 0.514
4.761 ± 0.448
10.777 ± 1.091
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.792 ± 0.154
4.588 ± 0.333
4.804 ± 0.538
4.458 ± 0.415
5.129 ± 0.536
9.046 ± 0.597
6.557 ± 0.39
6.47 ± 0.621
0.844 ± 0.151
3.419 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here