Zambian malbrouck virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Simarterivirinae; Epsilonarterivirus; Sheartevirus; Epsilonarterivirus zamalb

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167L777|A0A167L777_9NIDO ORF4' protein OS=Zambian malbrouck virus 1 OX=2682610 GN=ORF4' PE=4 SV=1
MM1 pKa = 6.8VFCWLLYY8 pKa = 10.74ACLSCILFQCVCCSNNITNSSSAEE32 pKa = 4.19VPTPTCFSFPRR43 pKa = 11.84ANFSVHH49 pKa = 4.65MHH51 pKa = 6.29FEE53 pKa = 4.03ALVCKK58 pKa = 9.78MDD60 pKa = 3.63GARR63 pKa = 11.84TIGSGRR69 pKa = 11.84TSASTGGCSSVANGEE84 pKa = 4.1GRR86 pKa = 11.84WPDD89 pKa = 3.39RR90 pKa = 11.84SILQYY95 pKa = 9.62PHH97 pKa = 7.27NITADD102 pKa = 3.29FDD104 pKa = 5.01LNNTLDD110 pKa = 3.34QSHH113 pKa = 5.86AHH115 pKa = 6.09IAALLTAVLTYY126 pKa = 10.72DD127 pKa = 3.9PEE129 pKa = 4.94AFNLNPNYY137 pKa = 9.9TRR139 pKa = 11.84GFNVSTDD146 pKa = 3.21EE147 pKa = 3.92KK148 pKa = 10.92HH149 pKa = 5.78YY150 pKa = 8.59TFCVNGTILLEE161 pKa = 4.1NSTLGSYY168 pKa = 10.62YY169 pKa = 10.34FFNPHH174 pKa = 4.92TWDD177 pKa = 3.65LYY179 pKa = 9.65ILEE182 pKa = 4.49LFRR185 pKa = 11.84PFVLSLLLLSISFAA199 pKa = 4.06

Molecular weight: 22.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167L7D6|A0A167L7D6_9NIDO N protein OS=Zambian malbrouck virus 1 OX=2682610 GN=ORF7 PE=4 SV=1
MM1 pKa = 7.69AGKK4 pKa = 9.61QNRR7 pKa = 11.84QSRR10 pKa = 11.84TPQSKK15 pKa = 8.71PRR17 pKa = 11.84RR18 pKa = 11.84PRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84PPQQSQSHH30 pKa = 5.01QNRR33 pKa = 11.84KK34 pKa = 6.33QHH36 pKa = 5.75EE37 pKa = 4.12PSFVFAAPDD46 pKa = 3.7DD47 pKa = 4.27LRR49 pKa = 11.84MKK51 pKa = 10.88LSVPNSKK58 pKa = 10.53EE59 pKa = 3.56ILRR62 pKa = 11.84LVCDD66 pKa = 4.62LFNHH70 pKa = 6.85GGGNLSYY77 pKa = 9.89EE78 pKa = 4.17NGYY81 pKa = 10.19ISYY84 pKa = 9.98QAAIAPYY91 pKa = 8.96GQLLRR96 pKa = 11.84AIQKK100 pKa = 10.45LSS102 pKa = 3.11

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5250

59

2075

437.5

48.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.8 ± 0.548

3.41 ± 0.16

4.514 ± 0.541

3.314 ± 0.191

4.876 ± 0.392

6.667 ± 0.559

3.143 ± 0.42

5.314 ± 0.399

4.133 ± 0.449

10.381 ± 0.657

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.581 ± 0.128

3.467 ± 0.359

6.61 ± 0.628

2.952 ± 0.291

4.667 ± 0.242

6.8 ± 0.573

7.371 ± 0.349

7.162 ± 0.54

1.143 ± 0.166

3.695 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski